MADH7 (SMAD7) (NM_005904) Human Untagged Clone

CAT#: SC116437

SMAD7 (untagged)-Human SMAD family member 7 (SMAD7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MADH7
• Synonyms: CRCS3; MADH7; MADH8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116437 sequence for NM_005904 edited (data generated by NextGen Sequencing)
  ATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGC
  GGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAA
  GGGGCGACGGACAGCCGAGCGCATGGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGC
  TGCCTGGGCAAGGCGGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCG
  GGCGCCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTC
  AAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGTGGAGTCCCGCGGCGGG
  ACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCCTGGACTGCAGGCTGGGCCCGGGGGCG
  CCCGCCGGCGCGCAGCCTGCGCAGCCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGC
  AAAGTGTTCAGGTGGCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGT
  GAATCTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTTAGCCGA
  CTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCCGATGGATTTTCTCAAA
  CCAACTGCAGACTGTCCAGATGCTGTGCCTTCCTCCGCTGAAACAGGGGGAACGAATTAT
  CTGGCCCCTGGGGGGCTTTCAGATTCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACAC
  TGGTGCGTGGTGGCATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAG
  GAGCCCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTCGGACAG
  CTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAGCAAAATCGGCTGCGGC
  ATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGGTGTACAACCGCAGCAGTTACCCCATC
  TTCATCAAGTCCGCCACACTGGACAACCCGGACTCCAGGACGCTGTTGGTACACAAGGTG
  TTCCCCGGTTTCTCCATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCC
  AATGACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGCTTTGTG
  AAGGGCTGGGGCCAGTGCTACACCCGCCAGTTCATCAGCAGCTGCCCGTGCTGGCTAGAG
  GTCATCTTCAACAGCCGGTAG
  
  Clone variation with respect to NM_005904.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005904 unedited
  GCGTAATTCGCAGAGGCGCGCGCCCCGGCCTCCGGGAGACTGGCGCATGCCACGGAGCGC
  CCCTCGGGCCGCCGCCGCTCCTGCCCGGGCCCCTGCTGCTGCTGCTGTCGCCTGCGCCTG
  CTGCCCCAACTCGGCGCCCGACTTCTTCATGGTGTGCGGAGGTCATGTTCGCTCCTTAGC
  AGGCAAACGACTTTTCTCCTCGCCTCCTCGCCCCGCATGTTCAGGACCAAACGATCTGCG
  CTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCA
  GGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT
  GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGCGGTGCGAGGT
  GCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCGCCGGCGCGGCCGGGGGCGCC
  GAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTCAAGAACTGAAGGAGCGGCAGCTGG
  AGCTGCTGCTCCAGCCGGTGGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGC
  CCGGCCGCCTGGACTGCANGCTGGCCCCGGGGGGCGCCCGCCGGCGCGCAGNCTGCGCAG
  ACGACCCTCGTCCTACTCGCTCCCCCTTCTGCTGTGNAAAGTGGTNCAAGGGGGCCGGAT
  CTCAGGCATTCTTCGGAAGTCAAAAGGCTGGGNTTGCTGCGAATCCTACCGGGAAGATCA
  ACCCGAACCCTGTGGTGCTGGACCCCCCTCACCTTAACCCGACTCTGCGAACTAAAATCT
  CCCCCCCCTCCTTACTTCAGATACCGGGAGTGAATTTCTTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005904 unedited
  ACTTGTTTGCTTTTAAAAACGCCTTTGGGCCTAAACTCCTTTATAAAACATCAGCTAGGT
  GATAACACCCATAGAAAAAAACACCAATCTTGTGTTTATCTTTTTTAATTTGGCATTTGT
  TATTTGCATTTATATTAAAGCAAAGTGCATCTTTTCTTTATTTTTCTTGTTTATACACAT
  TGCACAATACATAAATAATGATGCTTATAAAACGTCTTTATATTTACAAGTAATAATATA
  TTTATATATAACATAAAATACATTTTTTTCTTTAATAAATCTCAGGTTTTTTTTCAGGAG
  TCCTTTCTCTCTCAAAGCACTACAATGCTAAATTTCCAATGAGAATGCTTCTCTTGTTCA
  TTTAAACCTTTGTAGTTAGAAAAATAGCTTATGTTAAAGTCTAAACATGCTCATTGAGCT
  AAGAACAGTGTCGAAGTATCATACGAGTGTATGAGTTGTAGAAGAAATGAAAGAAGAGTT
  AGGTGTCAGCCTAGGATGGTACCTTGGGTTATGACGGACCAAATCCTTCTTGGAGGGAAG
  GGGACTGCTAAGCATGTCCCTCCCAAGGACATCCCCGCTTGCTGGCCTAATAGCAGAGCT
  TAACACACAGGATGGGAGCAGGCAGTGCTGAGCCTGGTTTCTAAGTCCGGGAAAAACCTG
  ATGTCACCAAGGCCAGCCANGGGAAAAGCCTGAAGGGAAAGGCCCACTGGGTTGAGCAAT
  ACTGTGAAGGGGCTTGCCCCGGGCACCCCTTGGGAAGCCCATTCTTAGGGAGATCCACGA
  ACCAGATTGGCCAAAAAAAAAAGACCCTCCATTGTCAAGGGCTTGGCAGGAAGGAAATAA
  AACAAGGGTTGAAATAATTGGTTCTGGGTTGGACACTTTCAAGCCTCCTTGTTCAAAAAC
  CTGGGAACAGGCCCCGTCCTTGTTGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005904
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005904.2, NP_005895.1
• RefSeq Size: 3103 bp
• RefSeq ORF: 1281 bp
• Locus ID: 4092
• UniProt ID: O15105
• Cytogenetics: 18q21.1
• Domains: DWB, DWA, MH1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.