Neurogranin (NRGN) (NM_006176) Human Untagged Clone

CAT#: SC116291

NRGN (untagged)-Human neurogranin (protein kinase C substrate, RC3) (NRGN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Neurogranin
• Synonyms: hng; RC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116291 sequence for NM_006176 edited (data generated by NextGen Sequencing)
  ATGGACTGCTGCACCGAGAACGCCTGCTCCAAGCCGGACGACGACATTCTAGACATCCCG
  CTGGACGATCCCGGCGCCAACGCGGCCGCCGCCAAAATCCAGGCGAGTTTTCGGGGCCAC
  ATGGCGCGGAAGAAGATAAAGAGCGGAGAGCGCGGCCGGAAGGGCCCGGGCCCTGGGGGG
  CCTGGCGGAGCTGGGGTGGCCCGGGGAGGCGCGGGCGGCGGCCCCAGCGGAGACTAG
  
  Clone variation with respect to NM_006176.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006176 unedited
  NGTAGCGGTCAAATTTGTAAACGACTCATATGGGCGGCCGCGNAATTCGCACGAGNAGAG
  AGAGAGAGACCGGACCCGAGAGCAGAGCTGCTGTTTCGGCGCGGGTCGGCTGGCGGCCGA
  CTGCCCCAGAGCCCCCACCCGGCACCACACAGACCCCACCCCCGCCCTGCGCCAGCCTTC
  GTCCCCGCAGAGGACCCCCCGACACCAGCATGGACTGCTGCACCGAGAACGCCTGCTCCA
  AGCCGGACGACGACATTCTAGACATCCCGCTGGACGATCCCGGCGCCAACGCGGCCGCCG
  CCAAAATCCAGGCGAGTTTTCGGGGCCACATGGCGCGGAAGAAGATAAAGAGCGGAGAGC
  GCGGCCGGAAGGGCCCGGGCCCTGGGGGGCCTGGCGGAGCTGGGGTGGCCCGGGGAGGCG
  CGGGCGGCGGCCCCAGCGGAGACTAGGCCAGAACTGAGCATTTTCAAAGTTCCCGAGGAG
  AGATGGATGCCGCGTCCCCTTCGCAGCGACGAGACTTCCCTGCCGTGTTTGTGACCCCCT
  CCTGCCCAGCAACCTGCCAGCTACAGGAGCCCCCTGCGTCCCAGAGACTCCCTCACCCAG
  GCAGGCTCCGTCGCGGAGTCGCTGAGTCCGTGCCCTTTTAGTTAGTTCTGCAGTCTAGTA
  TGGTCCCCATTTGCCCTTCCACTCCACCCCACCCTAAACCATGCGCTCCCAATCTTCCTT
  CTTTTGCTTCTCGCCCACCTCTTCCCGCACCCAGCATGCAGCTCTGCCTCCGCAGCCTCA
  GTGCGCTTTCCTGCGCGCACTGCGGAGGGCGCCCTAAGCGTCACCCAAGCACACTCACTT
  AAAGGAAAAACGAGTTTCTTTCGTTCTGTGCGCAGCTAAAAAGGGCGCCCTACATCTCCG
  TGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006176 unedited
  ATGGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGCATTCCTCT
  TTATTGTTCACATTCCAACCCAGCACAGTCACATGCACACACGGAGATCAGAAACCTTTC
  GGCCACAGCCCCAGGAGCCCGGCGGGGGGGAGGGCGGGACCGACAGGGGCGGGGCGGGGC
  CGTGGAAGACTCCTCCTACCGAGCCTCCCAGGCGCTCGGCGTTTGCATAAACAAGAGAGC
  TGGAGAGGCTGCCCTCAACAGTGCGCTGGGGAAAGGGGAGGGAACGTGACAGGCAGGTGT
  GGGATAGGGACTTCTCTTCCGGTCAGAGCAAGGGTCGTCCGAAACCAAAACAACCCTCTT
  CCCTTCATCTCGCCCCGGATCCAAAGTCTTGGGGCTAGGCTGGGGCGGGAGTGGCACGGA
  GATGTAGGGCGCCCCTTTTAGCTGCGCACAGAACGAAAGAACTCGTTTTTTCTTTAAGTG
  AGTGTGCTTGGGTGACGCTTAGGGCGCCCTCCGCAGTGCGCGCAGGAAAGCGCACTGAGG
  CTGCGGAGGCAGAGCTGCATGCTGGGTGCGGGAAGAGGTGGGCGAGAAGCAAAAGAAGGA
  AGATTGGGAGCGCATGNGTTTAGGGTGGGGTGGAGTGGGAAGGGGCCAAAGGGGGACCAT
  ACTAGACTGCAGAACTAACTAAAAGGGCACGGGACTCAGCGACTCCGCGACGGAGCCCGC
  CTTGGTGGAGGGGAATCTTTGGACCCCACGGGGGCTCCTGTTACTGGCAAGTTGCTTGGC
  AGAAAGGGGTCCCAACCCCGCAGGGGAAAACTCTCCCCTGCGAAGGGGACCCGGAAAACA
  TTTTTCGCCGGGACACTTGAAAAGCCCAATACTTGGCCTAACATACCTTTGGGGACCACC
  CCCCCCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006176
• Insert Size: 1160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006176.1, NP_006167.1
• RefSeq Size: 1295 bp
• RefSeq ORF: 237 bp
• Locus ID: 4900
• UniProt ID: Q92686
• Cytogenetics: 11q24.2
• Domains: IQ
• Protein Families: Druggable Genome
• Gene Summary: Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) uses an alternate splice acceptor site in a 3' UTR exon.