AMPK alpha 2 (PRKAA2) (NM_006252) Human Untagged Clone

CAT#: SC116247

PRKAA2 (untagged)-Human protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPK alpha 2
• Synonyms: AMPK; AMPK2; AMPKa2; PRKAA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006252 edited
  GGCGGCCGCGCAATTCGCACGAGGCCGCGCGCGCCGAAGATGGCTGAGAAGCAGAAGCAC
  GACGGGCGGGTGAAGATCGGACACTACGTGCTGGGCGACACGCTGGGCGTCGGCACCTTC
  GGCAAAGTGAAGATTGGAGAACATCAATTAACAGGCCATAAAGTGGCAGTTAAAATCTTA
  AATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGAAATTCAAAAT
  CTAAAACTCTTTCGTCATCCTCATATTATCAAACTATACCAGGTGATCAGCACTCCAACA
  GATTTTTTTATGGTAATGGAATATGTGTCTGGAGGTGAATTATTTGACTACATCTGTAAG
  CATGGACGGGTTGAAGAGATGGAAGCCAGGCGGCTCTTTCAGCAGATTCTGTCTGCTGTG
  GATTACTGTCATAGGCATATGGTTGTTCATCGAGACCTGAAACCAGAGAATGTCCTGTTG
  GATGCACACATGAATGCCAAGATAGCCGATTTCGGATTATCTAATATGATGTCAGATGGT
  GAATTTCTGAGAACTAGTTGCGGATCTCCAAATTATGCAGCACCTGAAGTCATCTCAGGC
  AGATTGTATGCAGGTCCTGAAGTTGATATCTGGAGCTGTGGTGTTATCTTGTATGCTCTT
  CTTTGTGGCACCCTCCCATTTGATGATGAGCATGTACCTACGTTATTTAAGAAGATCCGA
  GGGGGTGTCTTTTATATCCCAGAATATCTCAATCGTTCTGTCGCCACTCTCCTGATGCAT
  ATGCTGCAGGTTGACCCACTGAAACGAGCAACTATCAAAGACATAAGAGAGCATGAATGG
  TTTAAACAAGATTTGCCCAGTTACTTATTTCCTGAAGACCCTTCCTATGATGCTAACGTC
  ATTGATGATGAGGCTGTGAAAGAAGTGTGTGAAAAATTTGAATGTACAGAATCAGAAGTA
  ATGAACAGTTTATATAGTGGTGACCCTCAAGACCAGCTTGCAGTGGCTTATCATCTTATC
  ATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCTAGTCCTCCA
  TCTGGTTCTTTTATGGATGATAGTGCCATGCATATTCCCCCAGGCCTGAAACCTCATCCA
  GAAAGGATGCCACCTCTTATAGCAGACAGCCCCAAAGCAAGATGTCCATTGGATGCACTG
  AATACGACTAAGCCCAAATCTTTAGCTGTGAAAAAAGCCAAGTGGCATCTTGGAATCCGA
  AGTCAGAGCAAACCGTATGACATTATGGCTGAAGTTTACCGAGCTATGAAGCAGCTGGAT
  TTTGAATGGAAGGTAGTGAATGCATACCATCTTCGTGTAAGAAGAAAAAATCCAGTGACT
  GGCAATTACGTGAAAATGAGCTTACAACTTTACCTGGTTGATAACAGGAGCTATCTTTTG
  GACTTTAAAAGCATTGATGATGAAGTAGTGGAGCAGAGATCTGGTTCCTCAACACCTCAG
  CGTTCCTGTTCTGCTGCTGGCTTACACAGACCAAGATCAAGTTTTGATTCCACAACTGCA
  GAGAGCCATTCACTTTCTGGCTCTCTCACTGGCTCTTTGACCGGAAGCACATTGTCTTCA
  GTTTCACCTCGCCTGGGCAGTCACACCATGGATTTTTTTGAAATGTGTGCCAGTCTGATT
  ACTACTTTAGCCCGTTGATCTGTCTCTAGTTTCTTTCTGTTATTGCACTATGAAAATCAG
  TTATATTCTTTAAATTTTTATCTTACTTTTGGATAATATCCACTGCAATACTAATTGAGA
  AACATGAATTATTTCCAGGGGCACACAATGCTATTGAAATTACTGAAAACAAAATATCTG
  ACATCTTATTTACTTGTAGAAATCTGTAATTCTATTGTGCCTATGATAAATTCACATAGG
  CAATATCTTTAATAGGTTAATATCAATGAAGATTTTTAATTACAATAATGAGTTCACTAC
  AGACGATTAACACACCACACTGGCGAACCATCTCAATGTAAGGGTGGTTTGGCAACACCT
  CCTTGCTTTGCTGTTTGGTGTAGGTAAATCTAGTTTACTTCCTAAATTTCAGTAGGCTTT
  ATGCTGTGTTTATGCCCCCAATTTATTTTAACAAAAGAAGATTAAAAAGTAAAAGAACCA
  CGAGTAAGATATTATTTAAATGTTGAAATCTTAAAAACCTGCCTCCAAGATTTCAGAAGC
  CAAGTTTTTCTAACAGTATTTGTACAAATACTGCCTAGTGTATTCAACAGAAGGACTGTG
  GGTCATGTAACAGGTAACCACAATTTTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006252 unedited
  NGCTTGTACCGCTAAAACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCCGCGCGC
  GCCGAAGAGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTGCTG
  GGCGACACGCTGGGCGTCGGCACCTTCGGCAAAGTGAAGATTGGAGAACATCAATTAACA
  GGCCATAAAGTGGCAGTTAAAATCTTAAATAGACAGAAGATTCGCAGTTTAGATGTTGTT
  GGAAAAATAAAACGAGAAATTCAAAATCTAAAACTCTTTCGTCATCCTCATATTATCAAA
  CTATACCAGGTGATCAGCACTCCAACAGATTTTTTTATGGTAATGGAATATGTGTCTGGA
  GGTGAATTATTTGACTACATCTGTAAGCATGGACGGGTTGAAGAGATGGAAGCCAGGCGG
  CTCTTTCAGCAGATTCTGTCTGCTGTGGATTACTGTCATAGGCATATGGTTGTTCATCGA
  GACCTGAAACCAGAGAATGTCCTGTTGGATGCACACATGAATGCCAAGATAGCCGATTTC
  GGATTATCTAATATGATGTCAGATGGTGAATTTCTGAGAACTAGTTGCGGATCTCCAAAT
  TATGCAGCACCTGAAGTCATCTCAGGCAGATTGTATGCAGGTCCTGAAGTTGATATCTGG
  AGCTGTGGTGTTATCTTGTATGCTCTTCTTTGTGGCACCCTCCCATTTGATGATGAGCAT
  GTACCTACGTTATTTAAGAAGATCCGAGGGGGTGTCTTTTATATCCCAGAATATCTCAAT
  CGTTCTGTCGCCACTCTCCTGATGCATATGCTGCAAGTTGACCCACTGAAACGAGCGACT
  ATCAAAGACATAAGAGAGCATGAAATGGGTTAANACAAGATTTGCCCAGTTACTTATTTT
  CTGGAGACCCTTCCTATGATGCTTACGTCAATGATGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006252 unedited
  NAATACTTGNACCGCGCCGCATTCTANATCGAGTTTTTTTCTTTTTTTTTTTTTGCTTAA
  AACACTGGTGTGTGAATATATATATTTATTTATTTAAGATAAAAATTTTTTCTTGATGTT
  CAAAGCTTCCCTGCCCTATCAGGTTCAACAGTAAAGGTCATAGCCAGCCCTTAGGGCCAG
  AACAGTGTTTTCAAACTTGGACTAGAAAAGATTCAAAGCTATATCAATCAACCACTCAGG
  ATCAAACAATGAACAAAGACAATAGTGACTAGGTGAGGTGAGGTGAAAGGAGTGGCTTCC
  AATGAAGAATTAGCATTATGAGCTGCACCAGGACCCTAACACCTAACACAGAAAATAAAG
  ATCAAAGATAAGGATTCTCATTTAAGGGACTGTTGATACTTTGCTTTGTTTCATTTAACT
  ATTTTATCACTGTTGGTGAAATGCAAAAATAAATAGAGGCAAAAAGAATTTGAGAAAGGT
  AGATTTTGAAGACATTTTTTAAATGACGTAAGTTTATGTAAAAGACAAAAGTTGTTGCAA
  TTCCAGATGTTCCAATATTAATTTGAAACTTGAAGGCTCTTAAGATAAATATATTATTCA
  GGGAAATCTCAAGATAAAAATCCTGAGTTAGTTACAGCTGTTTTTAAGAAACCTGAAAAT
  TGTGGTTACCTGTTACATGACCACAGTCCTTCTGTTGAATACACTAGGCAGTATTTGTAC
  AAATACTGTTAGAAAAACTTGGCTTCTGAAATCTTGGAAGCAGGTTTTTTAGATTTCAAC
  ATTTTAATAATATCTTACTCGTGGTTCTTTTACCTTTTAAACTTCCTTTGTTAAATAAAT
  TGGGGGCATAAACCCCGCATAAAGCCCACTGAAATTTAGGAAGTTAACTAGATTTACCTA
  CNCCCAACACCAAACAGGGAGGTGTTGCACAACCACCTTACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006252
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006252.2, NP_006243.2
• RefSeq Size: 2435 bp
• RefSeq ORF: 1659 bp
• Locus ID: 5563
• UniProt ID: P54646
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy
• Gene Summary: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]