RPB11 (POLR2J) (NM_006234) Human Untagged Clone

CAT#: SC116237

POLR2J (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPB11
• Synonyms: hRPB14; POLR2J1; RPB11; RPB11A; RPB11m
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006234 edited
  ATGAACGCCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGAAGATCACC
  ATTAACAAGGACACCAAGGTACCCAATGCCTGTTTATTCACCATCAACAAAGAAGACCAC
  ACACTGGGAAACATCATTAAATCACAACTCCTAAAAGACCCGCAAGTGCTATTTGCTGGC
  TACAAAGTCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGTGCAGACCACGCCGGAC
  TACAGCCCCCAGGAAGCCTTTACCAACGCCATCACCGACCTCATCAGTGAGCTGTCCCTG
  CTGGAGGAGCGCTTTCGGGTGGCCATAAAAGACAAGCAGGAAGGAATTGAGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006234 unedited
  CACGAGGCGGCGGCGGCGGACCCTTGGGGTCTGGACGCAACGGCGGCGGGAGCATGAACG
  CCCCTCCAGCCTTCGAGTCGTTCTTGCTCTTCGAGGGCGAGAAGAAGATCACCATTAACA
  AGGACACCAAGGTACCCAATGCCTGTTTATTCACCATCAACAAAGAAGACCACACACTGG
  GAAACATCATTAAATCACAACTCCTAAAAGACCCGCAAGTGCTATTTGCTGGCTACAAAG
  TCCCCCACCCCTTGGAGCACAAGATCATCATCCGAGTGCAGACCACGCCGGACTACAGCC
  CCCAGGAAGCCTTTACCAACGCCATCACCGACCTCATCAGTGAGCTGTCCCTGCTGGAGG
  AGCGCTTTCGGGTGGCCATAAAAGACAAGCAGGAAGGAATTGAGTAGGGGCCAGAGGGGG
  CTCTGCTCGGCCTGTGAGCCCCGTTCCTACCTGTGCCTGACCCTCCGCTCCAGGTACCAC
  ACCGAGGAGAGCGGCCGGTCCCAGCCATGGCCCGCCTTGTGGCCACCCCTCACCCTGACA
  CCGACGTGTTGGCCACCCCTCACCCTGACACCGACGTGTCCTGTACATAGATTANGTTTT
  ATATTCCTAATAAAGTATAGCGGGAGAGAAAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006234 unedited
  GGATGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTCTCTCCCGCTATACTTTATTAGGAATATAAAACCTAATCTATGTCCAGGACACTTCGG
  TGTCAGGGGGAGGGGGGGCCAACACCTCGGTGTCAGGGGGAGGGGGGGCCACAAGGCGGC
  CCATGGCTGGAACCGTCTTTTTTCCTCGGGGGGGAACCTGGAGCGGAGGGTCAGGCACAG
  GTAGAAACGGGGCTCACAGGCCAAACAAAACCCCCTTTGGCCCCTACTAAATTCCTTCCT
  GCTTGGCTTTTATGGCCACCCGAAAGCGCTCCTCCAGCAGGGACAGCTCACTGATAAGGT
  CGGTGATGGCGTTGGAAAAGGCTTCCTGGGGGCTGTAGTCCGGCGTGGTCTGCACTCGGA
  TAATGATCTTGGGCTCCAAGGGGGGGGGGACTTTGTACCCACCAAATAACACTTGCGGGT
  CTTTTAGGAATTGTGATTAAATGATGCTTCCCAGGGTGGGGCCTCCTTTGTTGATGGTGA
  ATAAACAGGCATTGGGAACCTTGGTGCCCTTGTTATGGAGACCTTTTCTCCCCTCCAAGA
  CAAGAACACCTCAAGGCTGGAGGGGCGTTAAGCCTCCCCCCGCGTTGCGTCCAAACCCAA
  GGGTCCCCCCCCCCCCCCCTTTCCCAAATCGGGGCCGCCCTTATAGGAATCGTATTACAA
  AATTTTGACGGGTCACTTAAAAAACCTTTGTTTAATTAACCTTCCACCGGAACCGCTACC
  GCCTATTTGCTAAACGGGGCGGGGTTTTAACAACCTTTGGAAAGCCCCTTGTTTTTTGGG
  CCCAACAACACTCACTTCACTCCATGGGGGGGACACCTTGAAATCCCCTGAGGTAACCGC
  TTTCACCGCCCTTGGTGGTCTGGCACAACCTCTCCCGTGTGTTAAGGCGAAATAATCTCT
  TAATTTCTCCCAATACAAAAACCCCAAGTGTCTTCTCTGGCATAAGCAACCCGACCTTAC
  CCATAGAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006234
• Insert Size: 760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006234.4, NP_006225.1
• RefSeq Size: 991 bp
• RefSeq ORF: 354 bp
• Locus ID: 5439
• UniProt ID: P52435
• Cytogenetics: 7q22.1
• Domains: RNA_pol_L
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]