iASPP (PPP1R13L) (NM_006663) Human Untagged Clone

CAT#: SC115944

PPP1R13L (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 13 like (PPP1R13L), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: iASPP
• Synonyms: IASPP; NKIP1; RAI; RAI4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006663, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACAGCGAGGCATTCCAGAGCGCGCGGGACTTTCTGGACATGAACTTCCAGTCGCTGGCCATGAAAC
  ACATGGATCTGAAGCAGATGGAGCTGGACACGGCGGCGGCCAAGGTGGATGAACTGACCAAGCAGCTGGA
  GTCGCTGTGGTCAGACTCTCCCGCGCCTCCTGGCCCGCAGGCCGGACCCCCTTCTAGGCCGCCCCGGTAC
  AGCTCCAGCTCGATCCCTGAGCCCTTCGGCAGCCGAGGGTCCCCCCGGAAGGCGGCCACCGACGGCGCAG
  ACACCCCGTTCGGACGATCAGAGAGTGCCCCAACCCTACACCCCTACAGCCCGCTGTCCCCCAAGGGACG
  GCCGTCGTCGCCGCGCACCCCGCTCTACCTGCAGCCGGACGCCTACGGCAGCCTGGACCGCGCGACCTCG
  CCCCGGCCCCGCGCCTTCGATGGCGCAGGCAGCTCCCTCGGCCGTGCGCCCTCCCCGCGGCCCGGGCCAG
  GCCCGCTCCGCCAGCAGGGTCCCCCCACGCCTTTCGACTTCCTGGGCCGCGCAGGCTCCCCCCGCGGCAG
  CCCCCTGGCGGAGGGGCCCCAGGCCTTCTTCCCCGAGCGTGGGCCGTCACCGCGCCCCCCTGCCACAGCC
  TACGACGCGCCAGCGTCCGCCTTCGGGAGCTCCCTGCTAGGCTCCGGCGGCAGCGCATTCGCCCCGCCTC
  TGCGCGCGCAAGACGACCTGACGCTGCGCCGGCGGCCTCCGAAAGCCTGGAACGAGTCTGACCTGGACGT
  GGCGTACGAGAAGAAGCCTTCGCAGACAGCGAGCTATGAACGCCTGGACGTCTTCGCAAGGCCTGCCTCG
  CCGAGCCTGCAGCTGTTGCCTTGGAGGGAGAGCAGCCTGGATGGACTGGGGGGCACCGGCAAGGACAACC
  TCACTAGCGCCACCCTGCCGCGCAATTACAAGGTCTCTCCTCTGGCCAGCGACCGGCGTTCAGACGCGGG
  CAGCTACCGGCGCTCGCTGGGCTCCGCGGGGCCGTCGGGCACTTTGCCTCGCAGCTGGCAGCCCGTCAGC
  CGCATCCCCATGCCCCCCTCCAGCCCCCAGCCCCGCGGGGCCCCGCGCCAGCGTCCCATCCCCCTCAGCA
  TGATCTTCAAGCTGCAGAACGCCTTCTGGGAGCACGGGGCCAGCCGCGCCATGCTCCCTGGGTCCCCCCT
  CTTCACCCGAGCACCCCCGCCTAAGCTGCAGCCCCAACCACAACCACAGCCCCAGCCACAATCACAACCA
  CAGCCCCAGCTGCCCCCACAGCCCCAGACCCAACCCCAAACCCCTACCCCAGCCCCCCAGCATCCCCAAC
  AGACATGGCCCCCTGTGAACGAAGGACCCCCCAAACCCCCCACCGAGCTGGAGCCTGAGCCGGAGATAGA
  GGGGCTGCTGACACCAGTGCTGGAGGCTGGCGATGTGGATGAAGGCCCTGTAGCAAGGCCTCTCAGCCCC
  ACGAGGCTGCAGCCAGCACTGCCACCGGAGGCACAGTCGGTGCCCGAGCTGGAGGAGGTGGCACGGGTGT
  TGGCGGAAATTCCCCGGCCCCTCAAACGCAGGGGCTCCATGGAGCAGGCCCCTGCTGTGGCCCTGCCCCC
  TACCCACAAGAAACAGTACCAGCAGATCATCAGCCGCCTCTTCCATCGTCATGGGGGGCCAGGGCCCGGG
  GGGCCGGAGCCAGAGCTGTCCCCCATCACTGAGGGATCTGAGGCCAGGGCAGGGCCCCCTGCTCCTGCCC
  CACCAGCTCCCATTCCACCCCCGGCCCCGTCCCAGAGCAGCCCACCAGAGCAGCCGCAGAGCATGGAGAT
  GCGCTCTGTGCTGCGGAAGGCGGGCTCCCCGCGCAAGGCCCGCCGCGCGCGCCTCAACCCTCTGGTGCTC
  CTCCTGGACGCGGCGCTGACCGGGGAGCTGGAGGTGGTGCAGCAGGCGGTGAAGGAGATGAACGACCCGA
  GCCAGCCCAACGAGGAGGGCATCACTGCCTTGCACAACGCCATCTGCGGCGCCAACTACTCTATCGTGGA
  TTTCCTCATCACCGCGGGTGCCAATGTCAACTCCCCCGACAGCCACGGCTGGACACCCTTGCACTGCGCG
  GCGTCGTGCAACGACACAGTCATCTGCATGGCGCTGGTGCAGCACGGCGCTGCAATCTTCGCCACCACGC
  TCAGCGACGGCGCCACCGCCTTCGAGAAGTGCGACCCTTACCGCGAGGGTTATGCTGACTGCGCCACCTA
  CCTGGCAGACGTCGAGCAGAGTATGGGGCTGATGAACAGCGGGGCAGTGTACGCTCTCTGGGACTACAGC
  GCCGAGTTCGGGGACGAGCTGTCCTTCCGCGAGGGCGAGTCGGTCACCGTGCTGCGGAGGGACGGGCCGG
  AGGAGACCGACTGGTGGTGGGCCGCGCTGCACGGCCAGGAGGGCTACGTGCCGCGGAACTACTTCGGGCT
  GTTCCCCAGGGTGAAGCCTCAAAGGAGTAAAGTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006663 unedited
  TATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATCGGCACGAGGGCCTTCTGGGA
  GCACGGGGCCAGCCGCGCCATGCTCCCTGGGTCCCCCCTCTTCACCCGAGCACCCCCGCC
  TAAGCTGCAGCCCCAACCACAACCACAGCCCCAGCCACAATCACAACCACAGCCCCAGCT
  GCCCCCACAGCCCCAGACCCAACCCCAAACCCCTACCCCAGCCCCCCAGCATCCCCAACA
  GACATGGCCCCCTGTGAACGAAGGACCCCCCAAACCCCCCACCGAGCTGGAGCCTGAGCC
  GGAGATAGAGGGGCTGCTGACACCAGTGCTGGAGGCTGGCGATGTGGATGAAGGCCCTGT
  AGCAAGGCCTCTCAGCCCCACGAGGCTGCAGCCAGCACTGCCACCGGAGGCACAGTCGGT
  GCCCGAGCTGGAGGAGGTGGCACGGGTGTTGGCGGAAATTCCCCGGCCCCTCAAACGCAG
  GGGCTCCATGGAGCAGGCCCCTGCTGTGGCCCTGCCCCCTACCCACAAGAAACAGTACCA
  GCAGATCATCAGCCGCCTCTTCCATCGTCATGGGGGGCCAGGGCCCGGGGGGCCGGAGCC
  AGAGCTGTCCCCCATCACTGAGGGATCTGAGGCCAGGGCAGGGCCCCCTGCTCCTGCCCC
  ACCAGCTCCCATTCCACCCCCGGCCCCGTCCCAGAGCAGCCCACCAGAGCAGCCGCAGAG
  CATGGAGATGCGCTCTGTGCTGCGGAAGGCGGGNCTCCCGCGCAAGGCCCGNCGNGCGCG
  CCTCACCCTNTGGTGCTCCTNCTGGCACGCGCGCTGACCGGNGAGCTGGAGGTGGTGCAN
  NCAGCCGGTGAAGAGATGAACGACCCGAGCCAGCCAACGAGGAGGCATCACTGTCTTGCA
  CAACGCATCTGCGCGCCAACTACTCTATCGTGGATTCCTCATCACGCGGGTGCCATGTTC
  ACTCCCCGAAGNCACGGTGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006663 unedited
  CCGCCCCAATTAGCTGATGNACGCGTCGGNCTCGCTATTTTACGGATCNGCTTTTTTTTT
  TTTTTTTTTCTGTGCAAACTACTTTTATTTATAAGGAAAGTTTCTCTATTTTGTTTATAA
  ACATTAAACCAGTGCTGTGTGAAGGCACTTAATTGGGGAGAGGTGGGGACAGGGAATCTC
  TGGTAGAAGACCCAATGGTTTCTCCACCTCAGACCTCCAAAACTGCTGGGAGAGATGGTG
  TCAGCAGTGACTCCCAGGAATATCCAGTGGTGTGGTGGCCCATCCCAGGCCCGGCTGGGC
  AGGTGGCTGGCTTGCTGGGGGATGTGATGATGGTGGTAGGCATGGGAGGCACTTTGGACG
  GGATCTGATTTGGCAAAAGGAAGTGGTTTCCTGTCCCCAGTGATTTCCAGCCCTTCCCAG
  ACCTTCCACGGCTAAGGCAGATTACTAAATTTAAGGCTGGGGCCCTCCTTCTTCCCTGGA
  CTTCCAGGAGAACAGACAACCGGTGGCAAGGACCACCACCAACAGGGTGAGGGGTGCAGA
  TACCAGGCATCAAAAAACACAGGTACAGGTTTGGAGGGAAGGCAGGAATGCTTGTTTCTG
  TCAGCCTCCAAAACCTCCTTTTATCCTGCTAGACTTTACTCATTTGAGGCCTACCCCTGG
  GGAACAGACCAAAATAGTTCCGCGGCCCGTTTCCTCTTTGGCCGTTTCATGTCGTCTCCC
  ACCATCTAGTCTTCCTCGGCACTTCTCTTGCGCACACCGGTTCCCGTCTCCCCCTTCTTA
  ATCGACCCCTATTCCTTTATATCCATTTTCTTATACACAACCGCATCCATTCACCCCTCG
  TCATCCCTTTTATTCTCATCCCCCCTTTTTCTCTCTTTGTTCCTCTCACTCTATCTTTCT
  CCCCATTCTCCCCTTATCTCCTCCTTAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006663
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006663.1, NP_006654.1
• RefSeq Size: 3120 bp
• RefSeq ORF: 3120 bp
• Locus ID: 10848
• UniProt ID: Q8WUF5
• Cytogenetics: 19q13.32
• Domains: SH3, ANK
• Protein Families: Transcription Factors
• Gene Summary: IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.