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14-3-3 theta (YWHAQ) (NM_006826) Human Untagged Clone

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SKU
SC115879
YWHAQ (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol 14-3-3 theta
Synonyms 1C5; 14-3-3; HS1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115879 sequence for NM_006826 edited (data generated by NextGen Sequencing)
ATGGAGAAGACTGAGCTGATCCAGAAGGCCAAGCTGGCCGAGCAGGCCGAGCGCTACGAC
GACATGGCCACCTGCATGAAGGCAGTGACCGAGCAGGGCGCCGAGCTGTCCAACGAGGAG
CGCAACCTGCTCTCCGTGGCCTACAAGAACGTGGTCGGGGGCCGCAGGTCCGCCTGGAGG
GTCATCTCTAGCATCGAGCAGAAGACCGACACCTCCGACAAGAAGTTGCAGCTGATTAAG
GACTATCGGGAGAAAGTGGAGTCCGAGCTGAGATCCATCTGCACCACGGTGCTGGAATTG
TTGGATAAATATTTAATAGCCAATGCAACTAATCCAGAGAGTAAGGTCTTCTATCTGAAA
ATGAAGGGTGATTACTTCCGGTACCTTGCTGAAGTTGCGTGTGGTGATGATCGAAAACAA
ACGATAGATAATTCCCAAGGAGCTTACCAAGAGGCATTTGATATAAGCAAGAAAGAGATG
CAACCCACACACCCAATCCGCCTGGGGCTTGCTCTTAACTTTTCTGTATTTTACTATGAG
ATTCTTAATAACCCAGAGCTTGCCTGCACGCTGGCTAAAACGGCTTTTGATGAGGCCATT
GCTGAACTTGATACACTGAATGAAGACTCATACAAAGACAGCACCCTCATCATGCAGTTG
CTTAGAGACAACCTAACACTTTGGACATCAGACAGTGCAGGAGAAGAATGTGATGCGGCA
GAAGGGGCTGAAAACTAA

Clone variation with respect to NM_006826.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006826 unedited
TGGATTTTGTAATACGATTTACTATAGGGCGGCCGCGAATTCGCACGAGGCGGCCCCGGC
CCCGCGCCCGCCATGGAGAAGACTGAGCTGATCCAGAAGGCCAAGCTGGCCGAGCAGGCC
GAGCGCTACGACGACATGGCCACCTGCATGAAGGCAGTGACCGAGCAGGGCGCCGAGCTG
TCCAACGAGGAGCGCAACCTGCTCTCCGTGGCCTACAAGAACGTGGTCGGGGGCCGCAGG
TCCGCCTGGAGGGTCATCTCTAGCATCGAGCAGAAGACCGACACCTCCGACAAGAAGTTG
CAGCTGATTAAGGACTATCGGGAGAAAGTGGAGTCCGAGCTGAGATCCATCTGCACCACG
GTGCTGGAATTGTTGGATAAATATTTAATAGCCAATGCAACTAATCCAGAGAGTAAGGTC
TTCTATCTGAAAATGAAGGGTGATTACTTCCGGTACCTTGCTGAAGTTGCGTGTGGTGAT
GATCGAAAACAAACGATAGATAATTCCCAAGGAGCTTACCAAGAGGCATTTGATATAAGC
AAGAAAGAGATGCAACCCACACACCCAATCCGCCTGGGGCTTGCTCTTAACTTTTCTGTA
TTTTACTATGAGATTCTTAATAACCCAGAGCTTGCCTGCACGCTGGCTAANACGGCTTTT
GATGAGGCCATTGCTGAACTTGATACACTGAATGAAGACTCATACCAAGACAGCACCCTC
ATCATGCAGTTGCTTAGAGACNACCTAACACTTTGGACATCAGACAGTGCNAGAGAAGAA
TGTGATGNCGCAGAAGGGGCTGAAAACTANATCCATACAGGGTGTCATCCTTCCTCCTTC
ACAGAAACCTTTTTAACATCTNCATTCCTTATTCACTTGGATTTTCTATACAAAAAAACC
ATTCTGTGTATGGATCAACCGTTATAGCCTTTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006826 unedited
GGCATGGCACTTCCAGGNCCGGNANAGGCACTGGGGAAGGGTCACAGGGNATGCCCCCGG
GATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTT
TTTTTTTTTAATGGTTTATAAAATGTACTTTTATTTTATGTTACTCTGCTGTTACATAGG
GCATAACATTTTCACAAGGCTTTTTTGGGACTACAGTCAATGATTAGCAACACACAATAG
TGGTCCAACTCTCTAAATAACAATCACTAGACAAACTGGACACCCTCTCACATGTGCACA
AATCTGCATGGAAAAGTACTAAAGTTTTAGACTTGGACTTGTGTACTTTAATTTATTTCC
CCTTTCTAGTGTATTAAGAAATGACATGCACTTTAATTTGCCAAAAGCAATGCTTGTATT
CTGGCAGCAACATGCTACTTCTATCACATAGTAAAGTGAATACCAGAACTACAAAGGCAG
GAGGTGTAAGTGAATTTTATTGGAGGGGGAAGGTGGCAACTTAAACAGCAGCAAATAAAG
AGTGAATAAGGAAACTCCCTGTTGCCACAGATACACAAGACCTCCGTATGTGATACAGGA
GCCATTTCAATTTGTGACCCCTAGAAGAGATGGCAAGTGCTTTTCCATTCAATCTAATAC
TCCCGGATTCCTACTAAAAAGAATACTTAAGAACCTGGAAAAGTTGCTTACTGGAAGGAA
ACCCCCAAGAGAAGGGGAGGGAATGTAAAATTTAAAAAGTTTGGGGAATACTCTCTAAAT
GGAATAAATACTTTTTCTATTTTCACAAAAACCAAACCAGTCACTGCAAAACTGGTTAAA
TACTTAATACAGATCCTTTTTAGGCTTAAT
Restriction Sites NotI-NotI |
ACCN NM_006826
Insert Size 1820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006826.2, NP_006817.1
RefSeq Size 2166 bp
RefSeq ORF 738 bp
Locus ID 10971
UniProt ID P27348
Cytogenetics 2p25.1
Domains 14-3-3
Protein Families Druggable Genome
Protein Pathways Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection
Summary This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006826" in other vectors (7)
SKU Description Size Price
RC208646 YWHAQ (Myc-DDK-tagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ) 10 ug
$300.00
RC208646L1 Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ), Myc-DDK-tagged 10 ug
$600.00
RC208646L2 Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ), mGFP tagged 10 ug
$600.00
RC208646L3 Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ), Myc-DDK-tagged 10 ug
$600.00
RC208646L4 Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ), mGFP tagged 10 ug
$600.00
RG208646 YWHAQ (tGFP-tagged) - Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ) 10 ug
$489.00 $500.00
SC322035 YWHAQ (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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