ADAMTS1 (NM_006988) Human Untagged Clone

CAT#: SC115765

ADAMTS1 (untagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 1 (ADAMTS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAMTS1
• Synonyms: C3-C5; METH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115765 sequence for NM_006988 edited (data generated by NextGen Sequencing)
  ATGCAGCGAGCTGTGCCCGAGGGGTTCGGAAGGCGCAAGCTGGGCAGCGACATGGGGAAC
  GCGGAGCGGGCTCCGGGGTCTCGGAGCTTTGGGCCAGTACCCACGCTGCTGCTGCTCGCC
  GCGGCGCTACTGGCCGTGTCGGACGCACTCGGGCGCCCCTCCGAGGAGGACGAGGAGCTA
  GTGGTGCCGGAGCTGGAGCGCGCCCCGGGACACGGGACCACGCGCCTCCGCCTGCACGCC
  TTTGACCAGCAGCTGGATCTGGAGCTGCGGCCCGACAGCAGCTTTTTGGCGCCCGGCTTC
  ACGCTCCAGAACGTGGGGCGCAAATCCGGGTCCGAGACGCCGCTTCCGGAAACCGACCTG
  GCGCACTGCTTCTACTCCGGCACCGTGAATGGCGATCCCAGCTCGGCTGCCGCCCTCAGC
  CTCTGCGAGGGCGTGCGCGGCGCCTTCTACCTGCTGGGGGAGGCGTATTTCATCCAGCCG
  CTGCCCGCCGCCAGCGAGCGCCTCGCCACCGCCGCCCCAGGGGAGAAGCCGCCGGCACCA
  CTACAGTTCCACCTCCTGCGGCGGAATCGGCAGGGCGACGTCGGCGGCACGTGCGGGGTC
  GTGGACGACGAGCCCCGGCCGACTGGGAAAGCGGAGACCGAAGACGAGGACGAAGGGACT
  GAGGGCGAGGACGAAGGGGCTCAGTGGTCGCCGCAGGACCCGGCACTGCAAGGCGTAGGA
  CAGCCCACAGGAACTGGAAGCATAAGAAAGAAGCGATTTGTGTCCAGTCACCGCTATGTG
  GAAACCATGCTTGTGGCAGACCAGTCGATGGCAGAATTCCACGGCAGTGGTCTAAAGCAT
  TACCTTCTCACGTTGTTTTCGGTGGCAGCCAGATTGTACAAACACCCCAGCATTCGTAAT
  TCAGTTAGCCTGGTGGTGGTGAAGATCTTGGTCATCCACGATGAACAGAAGGGGCCGGAA
  GTGACCTCCAATGCTGCCCTCACTCTGCGGAACTTTTGCAACTGGCAGAAGCAGCACAAC
  CCACCCAGTGACCGGGATGCAGAGCACTATGACACAGCAATTCTTTTCACCAGACAGGAC
  TTGTGTGGGTCCCAGACATGTGATACTCTTGGGATGGCTGATGTTGGAACTGTGTGTGAT
  CCGAGCAGAAGCTGCTCCGTCATAGAAGATGATGGTTTACAAGCTGCCTTCACCACAGCC
  CATGAATTAGGCCACGTGTTTAACATGCCACATGATGATGCAAAGCAGTGTGCCAGCCTT
  AATGGTGTGAACCAGGATTCCCACATGATGGCGTCAATGCTTTCCAACCTGGACCACAGC
  CAGCCTTGGTCTCCTTGCAGTGCCTACATGATTACATCATTTCTGGATAATGGTCATGGG
  GAATGTTTGATGGACAAGCCTCAGAATCCCATACAGCTCCCAGGCGATCTCCCTGGCACC
  TCGTACGATGCCAACCGGCAGTGCCAGTTTACATTTGGGGAGGACTCCAAACACTGCCCC
  GATGCAGCCAGCACATGTAGCACCTTGTGGTGTACCGGCACCTCTGGTGGGGTGCTGGTG
  TGTCAAACCAAACACTTCCCGTGGGCGGATGGCACCAGCTGTGGAGAAGGGAAATGGTGT
  ATCAACGGCAAGTGTGTGAACAAAACCGACAGAAAGCATTTTGATACGCCTTTTCATGGA
  AGCTGGGGAATGTGGGGGCCTTGGGGAGACTGTTCGAGAACGTGCGGTGGAGGAGTCCAG
  TACACGATGAGGGAATGTGACAACCCAGTCCCAAAGAATGGAGGGAAGTACTGTGAAGGC
  AAACGAGTGCGCTACAGATCCTGTAACCTTGAGGACTGTCCAGACAATAATGGAAAAACC
  TTTAGAGAGGAACAATGTGAAGCACACAACGAGTTTTCAAAAGCTTCCTTTGGGAGTGGG
  CCTGCGGTGGAATGGATTCCCAAGTACGCTGGCGTCTCACCAAAGGACAGGTGCAAGCTC
  ATCTGCCAAGCCAAAGGCATTGGCTACTTCTTCGTTTTGCAGCCCAAGGTTGTAGATGGT
  ACTCCATGTAGCCCAGATTCCACCTCTGTCTGTGTGCAAGGACAGTGTGTAAAAGCTGGT
  TGTGATCGCATCATAGACTCCAAAAAGAAGTTTGATAAATGTGGTGTTTGCGGGGGAAAT
  GGATCTACTTGTAAAAAAATATCAGGATCAGTTACTAGTGCAAAACCTGGATATCATGAT
  ATCATCACAATTCCAACTGGAGCCACCAACATCGAAGTGAAACAGCGGAACCAGAGGGGA
  TCCAGGAACAATGGCAGCTTTCTTGCCATCAAAGCTGCTGATGGCACATATATTCTTAAT
  GGTGACTACACTTTGTCCACCTTAGAGCAAGACATTATGTACAAAGGTGTTGTCTTGAGG
  TACAGCGGCTCCTCTGCGGCATTGGAAAGAATTCGCAGCTTTAGCCCTCTCAAAGAGCCC
  TTGACCATCCAGGTTCTTACTGTGGGCAATGCCCTTCGACCTAAAATTAAATACACCTAC
  TTCGTAAAGAAGAAGAAGGAATCTTTCAATGCTATCCCCACTTTTTCAGCATGGGTCATT
  GAAGAGTGGGGCGAATGTTCTAAGTCATGTGAATTGGGTTGGCAGAGAAGACTGGTAGAA
  TGCCGAGACATTAATGGACAGCCTGCTTCCGAGTGTGCAAAGGAAGTGAAGCCAGCCAGC
  ACCAGACCTTGTGCAGACCATCCCTGCCCCCAGTGGCAGCTGGGGGAGTGGTCATCATGT
  TCTAAGACCTGTGGGAAGGGTTACAAAAAAAGAAGCTTGAAGTGTCTGTCCCATGATGGA
  GGGGTGTTATCTCATGAGAGCTGTGATCCTTTAAAGAAACCTAAACATTTCATAGACTTT
  TGCACAATGGCAGAATGCAGTTAA
  
  Clone variation with respect to NM_006988.3
  96 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006988 unedited
  GGGTGGTTAGGAACCGCCTTCATNAGAGGCGGACGCCCAATCGGCACGAGCGGCTCCGAT
  CCAGGGGCTATTGCAAAGCCAGGGCGCGCTACCGGACGGAGAGGGGAGAGCCCTGAGCAG
  AGTGAGCAACATCGCAGCCAAGGCGGAGGCCGAAGAGGGGCGCCAGGCACCAATCTCCGC
  GTTGCCTCAGCCCCGGAGGCGCCCCAAAGTGCTTCNTGTCCCAGCAGAGCCACTCTGCCT
  GCGCCTGCCTCTCAGTGTCTCCAACTTTGCGCTGGAAGAAAAACTTCCCGCGCGCCGGCA
  GAACTGCAGCGCCTCCTTTTAGTGACTCCGGGAGCTTCGGCTGTAGCCGGCTCTGCGCGC
  CCTTCCAACGAATAATAGAAATTGTCAATTTTAACAATCCAGAGCAGGCCAACGAGGCTT
  TGCTCTCCCGACCCGAACTAAAGGTCCCTCGCTCCGTGCGCTGCTACGAGCGGAGTCTCC
  TGGGGCTCCAATGCAGCGAGCTGTGCCCGAGGGGTTCGGAAGGCGCAAGCTGGGCAGCGA
  CATGGGGAACGCGGAGCGGGCTCCGGGGTCTCGGAGCTTTGGGCCAGTACCCACGCTGCT
  GCTGCTCGCCGCGGCGCTACTGGCCGTGTCGGACGCACTCGGGCGCCCCTCCGAGGAGGA
  CGAGGAGCTAGTGGTGCCGGAGCTGGAGCGCGCCCCGGGACACGGGACCACGCGCCTCCG
  CCTGCACGCCTTTGACCAGCAGCTGGATCTGGAGCTGCGGCCCGACAGCAGCTTTTGGCC
  GCCCGGCTTCACGCTCCAGAACGTGGGGCGCAAATCCGGGTCCGAGACGCCGCTTCCGGA
  AACCGACCTGTCGCACTGCCTCTACTTCGTCACCGTGAATGGNGATCCCAGCTCGGCTGG
  CGGCCCCAGCCTCTGCGAGACGGGCGCGGNGCCTNCTACCTGCTGGGGAGCGGATTCATC
  CACCCTGCCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006988 unedited
  CCAGTGGGGCCATAGTGGGATGGTCAACTTACCAGGTCCAGAAAAGCACTGGGGAAGGGG
  TCACAGGGATGCCACCCGGGCTCTGTTGCAGGAAAAGCTATGACCGCGGCCGCAATCTAA
  AGTCAAGTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTATATTCCCCAAAAGG
  GTTCTTTTTTTTCTTAAAACATATATTGTATAAATCCCCTTTTTTAAAACCCCTCCCACA
  AAAGCGATATTTTTTAAAAGTTTTTTCCATTAAAAATAAATGTCTAAAAATAAATTTGTT
  ATTAAAGGCATTTTTTACAAACTTTTGCCCTTTTTGGAAGGAACCCCCAACCCAATCCTT
  ATAAAGTCCATGTTATTTATAGTAACATTTTTTACTTTTTCCGTTTGGAAAAAATCTTTT
  TCTCCCAAAAAAACTTAACAAACTTTCCCCAGGATACCACTGTTGGACCACCTGTTGGAA
  ATGGACCTGGTACCCCTAAAAAGCCTCCCCACCAAAAAAAAAGTGATCTCAATGTCCCCA
  AACCTGGGGGACCCTGTTCTACACACCCTATTTTTTTTCCGGCCTTTCACCCCCCTGGGG
  GGATTGTCCCGATTTTCAAGAAACACAATTTACTTTTTTACTTCCCTATTCCCAAACCAG
  GGTTTCTTGGTAAAAAAAAAAAAAATTTGGAGGTACCAACCCTATTGGGGCCCCCTTAAC
  TTTTACCAAAAACAAAAGCTTCACCGCTTTTTTTCCATGAAAACTTTCATGCACTTGAAT
  AAAACCTGGGAGTTTTTTTTTCACCCCAATGGATTACCCCAATAAAGCCCCGGGTGGCCC
  CAAAATAGGAAAAGAAAACCCTTTGCCTTTTTTCACGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006988
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006988.3, NP_008919.3
• RefSeq Size: 4670 bp
• RefSeq ORF: 2904 bp
• Locus ID: 9510
• UniProt ID: Q9UHI8
• Cytogenetics: 21q21.3
• Domains: tsp_1, Reprolysin, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]