NDUFV1 (NM_007103) Human Untagged Clone

CAT#: SC115721

NDUFV1 (untagged)-Human NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa (NDUFV1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFV1
• Synonyms: CI-51K; CI51KD; MC1DN4; UQOR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115721 sequence for NM_007103 edited (data generated by NextGen Sequencing)
  ATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTCCCGCGCGGGTATCTGTGCGTTTC
  AGCGGCGACACGACAGCACCCAAGAAAACCTCATTTGGCTCGCTGAAGGATGAAGACCGG
  ATTTTCACCAACCTGTACGGCCGCCATGACTGGAGGCTGAAAGGTTCCCTGAGTCGAGGT
  GACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGCCCGACTGGATCCTGGGCGAGATC
  AAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCTTCCCCACTGGCCTCAAGTGGAGC
  TTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAACGCAGACGAGGGG
  GAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCCATGATCCTCACAAGCTGCTGGAA
  GGCTGCCTGGTGGGGGGCCGGGCCATGGGCGCCCGCGCTGCCTATATCTACATCCGAGGG
  GAATTCTACAATGAGGCCTCCAATCTGCAGGTGGCCATCCGAGAGGCCTATGAGGCAGGT
  CTGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGTTTGTGGTGCGCGGG
  GCTGGGGCCTACATCTGTGGAGAGGAGACAGCGCTCATCGAGTCCATTGAGGGCAAGCAG
  GGCAAGCCCCGCCTGAAGCCCCCCTTCCCCGCAGACGTGGGAGTGTTTGGCTGCCCCACA
  ACTGTGGCCAACGTGGAGACAGTGGCAGTGTCCCCCACAATCTGCCGCCGTGGAGGTACC
  TGGTTTGCTGGCTTTGGCAGAGAACGCAACTCAGGCACCAAACTATTCAACATCTCTGGC
  CATGTCAACCACCCTTGCACTGTGGAGGAGGAGATGTCTGTGCCCTTGAAAGAACTGATT
  GAGAAGCATGCTGGGGGTGTCACGGGCGGCTGGGACAACCTCCTTGCTGTGATCCCTGGC
  GGCTCGTCTACCCCACTGATCCCCAAGTCTGTGTGTGAGACGGTGCTGATGGACTTCGAT
  GCGCTGGTGCAGGCACAGACAGGCCTGGGCACAGCTGCGGTGATCGTCATGGACCGCTCG
  ACGGACATCGTGAAAGCCATCGCCCGCCTCATTGAGTTCTATAAGCACGAGAGCTGTGGC
  CAGTGTACCCCATGCCGTGAGGGTGTGGACTGGATGAACAAGGTGATGGCACGTTTCGTG
  AGGGGGGATGCCCGGCCGGCCGAGATCGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
  GGCCATACGATTTGTGCTCTGGGTGACGGGGCCGCCTGGCCTGTGCAGGGTCTGATCCGC
  CACTTTCGGCCGGAGCTCGAGGAGCGGATGCAGCGGTTTGCCCAGCAGCATCAGGCCCGG
  CAGGCTGCCTCTTAG
  
  Clone variation with respect to NM_007103.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007103 unedited
  GTTTCAGNATATTTGTATACCATCTCCTATAGGCGGCCGCCGAATTCGCACGAGGCGTGA
  GGTGACCCATCTGGCCCGCCGCGATGCTGGCAACACGGCGGCTGCTCGGCTGGTCGCTTC
  CCGCGCGGGTATCTGTGCGTTTCAGCGGCGACACGACAGCACCCAAGAAAACCTCATTTG
  GCTCGCTGAAGGATGAAGACCGGATTTTCACCTTTCTGTACGGCCGCCATGACTGGAGGC
  TGAAAGGTTCCCTGAGTCGAGGTGACTGGTACAAGACAAAGGAGATCCTGCTGAAGGGGC
  CCGACTGGATCCTGGGCGAGATCAAGACATCGGGTTTGAGGGGCCGTGGAGGCGCTGGCT
  TCCCCACTGGCCTCAAGTGGAGCTTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATC
  TGGTGGTGAACGCAGACGAGGGGGAGCCGGGCACCTGCAAGGACCGGGAGATCTTACGCC
  ATGATCCTCACAAGCTGCTGGAAGGCTGCCTGGCGGGGGGCCGGGCCATGGCCGCCCGCG
  CTGCCTATATCTACATCCGAGGGGAATTCTAACAATGAGGCCTCCAATCTGCACGCGGCC
  ATCCGAGAGGCCTATGAGGCAGGTCTGATTGCCAAGAATGCTTGCGGCTCTGGCTATGAC
  TTCGACGCGCTTGTGGTGCGCGGGGCTGGGGCCTACATCTGCGGCAAGGAGACAGCGCTC
  ATCCTACCCCTTGACGGCAAGAAGGCCAAGCCCCGCCTGAAGCCCCCCTTCCCCGAATAC
  CTGGGAGTGTTTGGCTGCCCCCCACTGTGGGCACCTGGAAACAGTGGCGGGGCCCCCACA
  ATCTGCCGCCGCGAAGTACCCGTTCTCCTGCTCTGGCCAAAACCCACCCAGCCCCAACTA
  TTCCAAAACTCGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007103 unedited
  AAACCACANCCCCCCCCCCCCCNNTAANNNNNNNAANNTTTGACTTTGNACCGCGGNCCG
  CATACTGNGATCGNGTTTTTTTTTTTTTTTTTTGGGAGGTGGGCAGCACTCGCTTNTATG
  TCCAGCATTCCACATGGGTAGACGCAGGACAGCAGGCCAGGGTGGTGGGCTAAGAGGCAG
  CCTGCCGGGCCTGATGCTGCTGGGCAAACCGCTGCATCCGCTCCTCGAGCTCCGGCCGAA
  AGTGGCGGATCAGACCCTGCACAGGCCAGGCGGCCCCGTCACCCAGAGCACAAATCGTAT
  GGCCTTCTATCTGCTTGCTGATCTCCCACAGGGAGTCGATCTCGGCCGGCCGGGCATCCC
  CCCTCACGAAACGTGCCATCACCTTGTTCATCCAGTCCACACCCTCACGGCATGGGGTAC
  ACTGGCCACAGCTCTCGTGCTTATAGAACTCAATGAGGCGGGCGATGGCTTTCACGATGT
  CCGTCGAGCGGTCCATGACGATCACCGCAGCTGTGCCCAGGCCTGTCTGTGCCTGCACCA
  GCGCATCGAAGTCCATCAGCACCGTCTCACACACAGACTTGGGGATCAGTGGGGTAGACG
  AGCCGCCAGGGATCACAGCAAGGAGGTTGTCCCAGCCGCCCGTGACACCCCCAGCATGCT
  TCTCAATCAGTTCTTTCAAGGGCACAGACATCTCCTCCTCCACAGTGCAGGGGTGGTTGA
  CATGGCCAGAGATGTTGAATAGTTTTGTGCCTGAGTTGCGTTCTCTGCCAAAGCCAGCAA
  ACCAGGTACCTTCACGGCGGCCAGATTGTGGGGGACACTGCCACTGTCTCCACGTNGCCA
  CAGTTGTGGGGCAGCCAAACACTCCACGTTTGNGGGGAAGGGGGGCTCAGCCGGGGCTTG
  CCCTNCTTGCCCTCAATGCATCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007103
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007103.2, NP_009034.2
• RefSeq Size: 1566 bp
• RefSeq ORF: 1395 bp
• Locus ID: 4723
• UniProt ID: P49821
• Cytogenetics: 11q13.2
• Domains: Complex1_51K
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10,000 live births. Mitochondrial complex I deficiency is linked to myopathies, encephalomyopathies, and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).