DGAT1 (NM_012079) Human Untagged Clone

CAT#: SC115583

DGAT1 (untagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGAT1
• Synonyms: ARAT; ARGP1; DGAT; DIAR7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012079, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGACCGCGGCAGCTCCCGGCGCCGGAGGACAGGGTCGCGGCCCTCGAGCCACGGCGGCGGCGGGC
  CTGCGGCGGCGGAAGAGGAGGTGCGGGACGCCGCTGCGGGCCCCGACGTGGGAGCCGCGGGGGACGCGCC
  AGCCCCGGCCCCCAACAAGGACGGAGACGCCGGCGTGGGCAGCGGCCACTGGGAGCTGAGGTGCCATCGC
  CTGCAGGATTCTTTATTCAGCTCTGACAGTGGCTTCAGCAACTACCGTGGCATCCTGAACTGGTGTGTGG
  TGATGCTGATCTTGAGCAATGCCCGGTTATTTCTGGAGAACCTCATCAAGTATGGCATCCTGGTGGACCC
  CATCCAGGTGGTTTCTCTGTTCCTGAAGGATCCCTATAGCTGGCCCGCCCCATGCCTGGTTATTGCGGCC
  AATGTCTTTGCTGTGGCTGCATTCCAGGTTGAGAAGCGCCTGGCGGTGGGTGCCCTGACGGAGCAGGCGG
  GACTGCTGCTGCACGTGGCCAACCTGGCCACCATTCTGTGTTTCCCAGCGGCTGTGGTCTTACTGGTTGA
  GTCTATCACTCCAGTGGGCTCCCTGCTGGCGCTGATGGCGCACACCATCCTCTTCCTCAAGCTCTTCTCC
  TACCGCGACGTCAACTCATGGTGCCGCAGGGCCAGGGCCAAGGCTGCCTCTGCAGGGAAGAAGGCCAGCA
  GTGCTGCTGCCCCGCACACCGTGAGCTACCCGGACAATCTGACCTACCGCGATCTCTACTACTTCCTCTT
  CGCCCCCACCTTGTGCTACGAGCTCAACTTTCCCCGCTCTCCCCGCATCCGGAAGCGCTTTCTGCTGCGA
  CGGATCCTTGAGATGCTGTTCTTCACCCAGCTCCAGGTGGGGCTGATCCAGCAGTGGATGGTCCCCACCA
  TCCAGAACTCCATGAAGCCCTTCAAGGACATGGACTACTCACGCATCATCGAGCGCCTCCTGAAGCTGGC
  GGTCCCCAATCACCTCATCTGGCTCATCTTCTTCTACTGGCTCTTCCACTCCTGCCTGAATGCCGTGGCT
  GAGCTCATGCAGTTTGGAGACCGGGAGTTCTACCGGGACTGGTGGAACTCCGAGTCTGTCACCTACTTCT
  GGCAGAACTGGAACATCCCTGTGCACAAGTGGTGCATCAGACACTTCTACAAGCCCATGCTTCGACGGGG
  CAGCAGCAAGTGGATGGCCAGGACAGGGGTGTTCCTGGCCTCGGCCTTCTTCCACGAGTACCTGGTGAGC
  GTCCCTCTGCGAATGTTCCGCCTCTGGGCGTTCACGGGCATGATGGCTCAGATCCCACTGGCCTGGTTCG
  TGGGCCGCTTTTTCCAGGGCAACTATGGCAACGCAGCTGTGTGGCTGTCGCTCATCATCGGACAGCCAAT
  AGCCGTCCTCATGTACGTCCACGACTACTACGTGCTCAACTATGAGGCCCCAGCGGCAGAGGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012079 unedited
  CACCATTATGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAGCAACGC
  AGCCGTTGTCCTTGAGGCCGACGGGCCTGACGCGGGCGGGTTGAACGCGCTGGTGAGGCG
  GTCACCCGGGCTACGGCGGCCGGCAGGGGGCAGTGGCGGCCGTTGTCTAGGGCCCGGAGG
  TGGGGCCGCGCGCCTCGGGCGCTACGAACCCGGCGGGCCCACGCTTGGCTGCGGCCGGGT
  GCGGGCTGAGGCCATGGGCGACCGCGGCAGCTCCCGGCGCCGGAGGACAGGGTCGCGGCC
  CTCGAGCCACGGCGGCGGCGGGCCTGCGGCGGCGGAAGAGGAGGTGCGGGACGCCGCTGC
  GGGCCCCGACGTGGGAGCCGCGGGGGACGCGCCAGCCCCGGCCCCCAACAAGGACGGAGA
  CGCCGGCGTGGGCAGCGGCCACTGGGAGCTGAGGTGCCATCGCCTGCAGGATTCTTTATT
  CAGCTCTGACAGTGGCTTCAGCAACTACCGTGGCATCCTGAACTGGTGTGTGGTGATGCT
  GATCTTGAGCAATGCCCGGTTATTTCTGGAGAACCTCATCAAGTATGGCATCCTGGTGGA
  CCCCATCCAGGTGGTTTCTCTGTTCCTGAAGGATCCCTATAGCTGGCCCGCCCCATGCCT
  GGTTATTGCGGCCAATGTCTTTGCTGTGGCTGCATTCCAGGTTGAGAAGCGCCTGGCGGT
  GGGTGCCCTGACCGAGCAGGCGGGACTGCTGCTGCACGTGGCCAACCTGGCCACCATTCT
  GTGGTTCCCCAGCGGCTGGGGTCCTACTGGTTGAGGCTATCACTCCAGNGGGCTCCCTGC
  TGCCGCTGATGCCGCACACCATTCTCTTTCTCAAGCTTTCTCCTACGCGACGCAAATTAT
  GTTGCCGCAAGGCCAGGCCAGGTTGCCTCTGCGGCAAAAGCCANCTGCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012079 unedited
  NTTTTGACTTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTGGCACTGGGCAGCACTTTATTGACACCCTCGGACCCGGGGCAGGGTCAGCAAGACTCC
  CAGCTGGCATCAAACTGTGTCTGGCCTGCTGTCGCCATCCCTGAGGGGTGCAGGACAGAG
  CCCCATAGGGGCAGAGAGGCCTCCCTGGGACCAGAGGAGGATGCTGTGCAGCCAGGCCCA
  TCCCCAGCACTCGAGGCCTAAGAGGAGAGGTGGGCTCTGGCAGCGGGTGTGAGGTGGCAG
  TGAGAAACCAGGCCCTCAGGTGCAGCTCAAGCCTCTGCCGCTGGGGCCTCATAGTTGAGC
  ACGTAGTAGTCGTGGACGTACATGAAGACGGCTATTGGCTGTCCGATGATGAGCGACAGC
  CACACAGCTGCGTTGCCATAGTTGCCCTGGAAAAAGCGGCCCACGAACCAGGCCAGTGGG
  ATCTGAGCCATCATGCCCGTGAACGCCCAGAGGCGGAACATTCGCAGAGGGACGCTCACC
  AGGTACTCGTGGAAGAAGGCCGAGGCCAGGAACACCCCTGTCCTGGCCATCCACTTGCTG
  CTGCCCCGTCGAAGCATGGGCTTGTAGAAGTGTCTGATGCACCACTTGTGCACAGGGATG
  TTCCAGTTCTGCCAGAAGTAGGTGACAGACTCGGAGTTCCACCAGTCCCCGTAGAACTCC
  CGGTCTCCAAACTGCATGAGCTCAGCCACGGCATTCANGCANGAGTGGAAGAGCCAGTAN
  AAAGAGATGAGCCAGATGAGGTGATTGGGGACCGCCAGCTTCANGAGGCGCTCGATGATG
  CGTGAGTAGTCCATGTCCCTGAANGGCCTCATGGNAGTCTGGATGGTGGGGACCATNCCT
  GCTGNATCACCCCNACTGNAGCTGGNTGAGACAGCATCTCAGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012079
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012079.2, NP_036211.1
• RefSeq Size: 1976 bp
• RefSeq ORF: 1467 bp
• Locus ID: 8694
• UniProt ID: O75907
• Cytogenetics: 8q24.3
• Domains: MBOAT
• Protein Families: Transmembrane
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways, Retinol metabolism
• Gene Summary: This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]