PHLDA3 (NM_012396) Human Untagged Clone

CAT#: SC115414

PHLDA3 (untagged)-Human pleckstrin homology-like domain, family A, member 3 (PHLDA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHLDA3
• Synonyms: TIH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115414 sequence for NM_012396 edited (data generated by NextGen Sequencing)
  ATGACGGCGGCGGCGACGGCTACCGTGCTCAAGGAGGGCGTGCTGGAGAAGCGCAGCGGC
  GGGCTGCTGCAGCTGTGGAAGCGGAAGCGCTGCGTCCTCACCGAACGCGGGCTGCAGCTC
  TTCGAGGCCAAGGGCACGGGCGGCCGGCCCAAGGAGCTCAGCTTCGCCCGCATCAAGGCC
  GTGGAGTGCGTGGAGAGCACCGGGCGCCACATCTACTTCACGCTGGTGACCGAAGGGGGC
  GGCGAGATCGACTTCCGCTGCCCCCTGGAAGATCCCGGCTGGAACGCCCAGATCACCCTA
  GGCCTGGTCAAGTTCAAGAACCAGCAGGCCATCCAGACAGTGCGGGCCCGGCAGAGCCTC
  GGGACCGGGACCCTCGTGTCCTAA
  
  Clone variation with respect to NM_012396.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012396 unedited
  AGTATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGGAACATGTAA
  GGGCACATCCCGCGAGCTGCCGCCCAGCGCGCAGACAGAGCCCAGGGGAGCAAGAGAACG
  GGCGGGCGGTGGGGCTCACGGCCTAGGGAGGCGCGGAGGCATCTGGCAGAGGCGGGTCGG
  GCTGGGCCAGCTGGGGTAGAGCGGAGGAGCGGGTGCCGGCTGAAGCGGGGCGGTGGGCGC
  GGAGCGCGCTGGGGGCACCGACACCACCTCACCGGCAGCCGGGTGCTGAGGGCCGCGGTG
  TGGGTGCGCGGAGCAGTCAGGGCGCAGGTGGGCAGCGCGCACGGCCTGCCAGCCCGGGGC
  GCCAGAATCCTGCGCTGCGGGGCCGAGAGGGGCGCCGCGCCCGCCGCAGCCTGGAGCTTT
  CCGCGAACCTCGGGGCGCCCATGACGGCGGCGGCGACGGCTACCGTGCTCAAGGAGGGCG
  TGCTGGAGAAGCGCAGCGGCGGGCTGCTGCAGCTGTGGAAGCGGAAGCGCTGCGTCCTCA
  CCGAACGCGGGCTGCAGCTCTTCGAGGCCAAGGGCACGGGCGGCCGGCCCAAGGAGCTCA
  GCTTCGCCCGCATCAAGGCCGTGGAGTGCGTGGAGAGCACCGGGCGCCACATCTACTTCA
  CGCTGGTGACCGAAGGGGGCGGCGAGATCGACTTCCGCTGCCCCCTGGAAGATCCCGGCT
  GGAACGCCCAGATCACCCTAGGCCTGGTCAAGTTCAAGAACCAGCAGGCCATCCAGACAG
  TGCGGGCCCGGCAGAGCCTCGGGACCGGGACCCTCGTGTCCTAAACCACCGGGCGCACCA
  TCTTTCCTTCATGCTACCCACCACCTCAGTGCTGAGGTCAAGCAGCTTTCTTTGTTCCTC
  TGGCTTGTGGGGGCACGGCTGTGCTCCATGTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012396
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012396.2, NP_036528.1
• RefSeq Size: 1607 bp
• RefSeq ORF: 384 bp
• Locus ID: 23612
• UniProt ID: Q9Y5J5
• Cytogenetics: 1q32.1
• Domains: PH
• Gene Summary: p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the functional protein. CCDS Note: This CCDS ID represents the protein described in PMID: 19203586 and is supported by BC014390.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 19203586. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein.