RPL13A (NM_012423) Human Untagged Clone

CAT#: SC115379

RPL13A (untagged)-Human ribosomal protein L13a (RPL13A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL13A
• Synonyms: L13A; TSTA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012423 edited
  ATGGCGGAGGTGCAGGTCCTGGTGCTTGATGGTCGAGGCCATCTCCTGGGCCGCCTGGCG
  GCCATCGTGGCTAAACAGGTACTGCTGGGCCGGAAGGTGGTGGTCGTACGCTGTGAAGGC
  ATCAACATTTCTGGCAATTTCTACAGAAACAAGTTGAAGTACCTGGCTTTCCTCCGCAAG
  CGGATGAACACCAACCCTTCCCGAGGCCCCTACCACTTCCGGGCCCCCAGCCGCATCTTC
  TGGCGGACCGTGCGAGGTATGCTGCCCCACAAAACCAAGCGAGGCCAGGCCGCTCTGGAC
  CGTCTCAAGGTGTTTGACGGCATCCCACCGCCCTACGACAAGAAAAAGCGGATGGTGGTT
  CCTGCTGCCCTCAAGGTCGTGCGTCTGAAGCCTACAAGAAAGTTTGCCTATCTGGGGCGC
  CTGGCTCACGAGGTTGGCTGGAAGTACCAGGCAGTGACAGCCACCCTGGAGGAGAAGAGG
  AAAGAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAG
  GCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCAAGACCCACGGA
  CTCCTGGTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012423 unedited
  AGGTCCTGGTGCTTGATGGTCGAGGCCATCTCCTGGGCCGCCTGGCGGCCATCGTGGCTA
  AACAGGTACTGCTGGGCCGGAAGGTGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTG
  GCAATTTCTACAGAAACAAGTTGAAGTACCTGGCTTTCCTCCGCAAGCGGATGAACACCA
  ACCCTTCCCGAGGCCCCTACCACTTCCGGGCCCCCAGCCGCATCTTCTGGCGGACCGTGC
  GAGGTATGCTGCCCCACAAAACCAAGCGAGGCCAGGCCGCTCTGGACCGTCTCAAGGTGT
  TTGACGGCATCCCACCGCCCTACGACAAGAAAAAGCGGATGGTGGTTCCTGCTGCCCTCA
  AGGTCGTGCGTCTGAAGCCTACAAGAAAGTTTGCCTATCTGGGGCGCCTGGCTCACGAGG
  TTGGCTGGAAGTACCAGGCAGTGACAGCCACCCTGGAGGAGAAGAGGAAAGAGAAAGCCA
  AGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACG
  TGGAGAAGAAAATTGACAAATACACAGAGGTCCTCAAGACCCACGGACTCCTGGTCTGAG
  CCCAATAAAGACTGTTAATTCCTNNNNNNNNNNNNNNNN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012423 unedited
  AGGAGAGGCACTGGGGAGGGGTACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGNCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAAGGAAATAACAGTTTTTATTGGGCTCAAACCAAGAGTCCGGGGGTC
  TTGAGGACCTCTGGGTATTTGGCAATTTTTTTTTCCACGTTTTTTTCGGCCTGTTTCCGT
  AACCTCATGAGCTGTTTCTTTTTCCGGGAGGGGATCTTGGCTTTCTCTTTCCTCTTCTCC
  TCCAGGGGGGCTGGCACTGCCTGGTACTTCCAGCCAACCTTGTGAGCCAGGCGCCCCAAA
  TAGGCAAACTTTCTTGTAGGCTTCAAACGCACGACCTTGAGGGCAGCAGGAACCACCATC
  CGCTTTTTCTTGTCGTAGGGCGGTGGGATGCCGTCAAACACCTTGAGACGGTCCAGAGCG
  GCCTGGCCTTGCTTGGTTTTTGTGGGGCAACATACCTTGCACGGTCCGCCAAAAATGCGG
  CTGGGGGCCCCGAAGTGTANGGGCCTNGGAAGGTTTTGGTGTCATCCCCTGGCGGAGAAA
  GCCCGTACCTTAACTGTTTTTGAAAAATGCCAAATTGTGGATGCTTTACAGGGACGACCC
  CACCTTCGGCCCAGATTACCTGTTCCCCCCAATGGCGCCAGGGGGCCAGAAAAAGGCTTT
  TACATAAACACCAGACCTGGCCTCGCCATTTTTCCTTTGGCCAATTTGCGGCCCCTCTAT
  TGGAGGTCTTTCACATATTTGGGGGCAATTAACAGCCCTGGTATTTACCCCTCCCCGACC
  CCCTCACCCCTTTTCGCCCGGGGGGGGTTTCTAATATTTCGCAGCCCCTCGCTTTTGGCC
  CACCACTCTCCCTCCCCGGGGGGGGCCATTCAACTCCCGGGCCACCCTTTCCCCCCTTGG
  TGGCCAACCCTTTTCGGTCAAGGATCAAATTTTTTCCCCCCCAAACCCGTTCCTCTCTCC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012423
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_012423.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012423.2, NP_036555.1
• RefSeq Size: 1142 bp
• RefSeq ORF: 612 bp
• Locus ID: 23521
• UniProt ID: P40429
• Cytogenetics: 19q13.33
• Domains: Ribosomal_L13
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.