PD-L1 (CD274) (NM_014143) Human Untagged Clone

CAT#: SC115168

PD-L1 / CD274 (untagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PD-L1
• Synonyms: B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115168 sequence for NM_014143 edited (data generated by NextGen Sequencing)
  ATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACT
  GTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGC
  AAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAG
  GATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGC
  TACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAG
  ATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGT
  GCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGA
  ATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTAC
  CCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACC
  ACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAAC
  ACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCAT
  ACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCAC
  TTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGT
  TTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAG
  AAGCAAAGTGATACACATTTGGAGGAGACGTAA
  
  Clone variation with respect to NM_014143.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014143 unedited
  CGGGCCCCNNNNCCCCAACCNCCCCCGGTTCAAATTTGTATACGACTCACTATAGGCGGC
  CGCGAATTCGCACGAGGCACCAGCCGCGCTTCTGTCCGCCTGCAGGGCATTCCAGAAAGA
  TGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTG
  TCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCA
  AATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGG
  ATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCT
  ACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGNGAAATGCTGCACTTCAGA
  TCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTG
  CCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAA
  TTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCANGCTGANGGCTACC
  CCAAGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCAC
  CACCAATTCCAAGAGAGAGGAGAAGCTTTTCATGTGACCACACACTGAGAAATCACACAN
  NCACTATGAGATTTTCTACTGCACTTTTANGAGAATAGATCCCTGAGAAAACATACAGCT
  GAATTGGTCATCCCAGAACTACCTCTGGCACATTCTTCAATGAAAGGACTCACTTGGTAA
  TNCTGGGAGCCATCCTATTATGCCCTTGGTGTAACACTGACATTCATTTTTNCGTTTAAG
  AAAGGNAGAATGATGNATGTGAAAAAATGTGCATNCCAGATACAANACTCAAGAAGCAAG
  TGATACACATTGGNAGGANACAGTATCCAGCATTGGAACTTCTGATCTTCAAGCGGGATN
  CTCAACCTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_014143 unedited
  TCGACCATGGAATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATG
  CCACCCGGGTTCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAAGTAACAAAATAAATAAAAATATT
  TTATTAAATTAATGCAGGTACAAAAAGTGACAATCAAATGCAAAATTAGGTATCATCTCT
  GCCTATGCCATTTACGATGAAACATGAGACAAAAGGGATAAAGTGCCTTACAAATCCAAC
  ACCACAGGGAGGAGTTAGGACTTAGGAATAGACTGAGTAGACTATGTGCCTTGCTCAGCC
  ACAATTCTTGCCTGTAATTCACACAAAGAACACTGTCACACCAATTACTGTACAAATGCA
  CATGTACAAACAGATAACACAAGGAGCTCTGTTGGAGACACTGTTTCTTCAGCCTTGACA
  TGTGGCAAAGCCAAGGTACTCCTTGTCATTGACACCAGAATATGGCCAAGAGGGAAAGGA
  AACTCTGGGATCTCCCAGGGCATCTGAATCTCGAAACCTCCAGGAAGCCTCTTAAGAGGG
  CTGTGTAGTGATGACAGCTGGTGGCATTCAAGGGTTCAAGCACAACGAATGAGGCTTTTC
  TGGCACAGGGTAGCTAGCAGTCAAGGTACACTGCCGGAATTTCCAGAAAGAAAATGTGAT
  TTTGCAAGTACAGCATCAAAGTGAAGTTTATACTTGACAGAAAGCAGAAAACAAAAGTTC
  CAGACATGATTCTGTCATACAGAGTTAATAATAAGATTGCTTTTTAGGACTAGATTGACT
  CAGTGCACCCTGGAGAGCCCATGGGTCTCCTTTTGAGCAACATAACAAATCCAACTCTGT
  CCTAGACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014143
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014143.2, NP_054862.1
• RefSeq Size: 1553 bp
• RefSeq ORF: 873 bp
• Locus ID: 29126
• UniProt ID: Q9NZQ7
• Cytogenetics: 9p24.1
• Domains: ig, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.