Hsp22 (HSPB8) (NM_014365) Human Untagged Clone

CAT#: SC115063

HSPB8 (untagged)-Human heat shock 22kDa protein 8 (HSPB8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Hsp22
• Synonyms: CMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014365 edited
  ATGGCTGACGGTCAGATGCCCTTCTCCTGCCACTACCCAAGCCGCCTGCGCCGAGACCCC
  TTCCGGGACTCTCCCCTCTCCTCTCGCCTGCTGGATGATGGCTTTGGCATGGACCCCTTC
  CCAGACGACTTGACAGCCTCTTGGCCCGACTGGGCTCTGCCTCGTCTCTCCTCCGCCTGG
  CCAGGCACCCTAAGGTCGGGCATGGTGCCCCGGGGCCCCACTGCCACCGCCAGGTTTGGG
  GTGCCTGCCGAGGGCAGGACCCCCCCACCCTTCCCTGGGGAGCCCTGGAAAGTGTGTGTG
  AATGTGCACAGCTTCAAGCCAGAGGAGTTGATGGTGAAGACCAAAGATGGATACGTGGAG
  GTGTCTGGCAAACATGAAGAGAAACAGCAAGAAGGTGGCATTGTTTCTAAGAACTTCACA
  AAGAAAATCCAGCTTCCTGCAGAGGTGGATCCTGTGACAGTATTTGCCTCACTTTCCCCA
  GAGGGTCTGCTGATCATCGAAGCTCCCCAGGTCCCTCCTTACTCAACATTTGGAGAGAGC
  AGTTTCAACAACGAGCTTCCCCAGGACAGCCAGGAAGTCACCTGTACCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014365 unedited
  ATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTTTATTAAGC
  TCCTGGCTCCGCTCTAGACCTCAGCGGTTCTGGCTGCCAGCCTGGGCAGCCTGGGAAGCC
  TGGGAGGACGGTGGCTTGCCGGTCTGTCGTGAGGCAGTGCGGACGGGGACCCTCTGGGAT
  TCTGCTGGATCTGCCCCGGGGGTTACCTTTGGGGGCTGGGACCCCAGTCGAGGGGACACA
  ACCGTCCCTGGCAGTGGTTGGTTCTGCTTCTCCCTGCAGAAAAGCAGCATTTTCGGAAGC
  TGAAGAATAAGCTAGCCCAGCCACACCACCTTGTTGTGTGACCTTGGGCAGGTGGTTCTG
  TCTCTCTGAGCCTCTGTTTCTCTCTGAGCTGAGCAGCCACCATGGCTGACGGTCAGATGC
  CCTTCTCCTGCCACTACCCAAGCCGCCTGCGCCGAGACCCCTTCCGGGACTCTCCCCTCT
  CCTCTCGCCTGCTGGATGATGGCTTTGGCATGGACCCCTTCCCAGACGACTTGACAGCCT
  CTTGGCCCGACTGGGCTCTGCCTCGTCTCTCCTCCGCCTGGCCAGGCACCCTAAGGTCGG
  GCATGGTGCCCCGGNGCCCCACTGCCACCGCCAGGTTTGGGGGTGCCTGCCGAGGGCAGG
  ACCCCCCCACCCTTCCCTGGGGAGCCCTGGAAAGTGTGTGTGAATGTGCACAGCTTCAAG
  CCAGAGGAGTTGATGGTGAAGACCAAAGATGGATACGTGGNAGGTGTCTGGNCAACATNG
  AAGAGAACAGCAAGAAGGTGGCATTGTTTCTAAGAACTTCACAAANAAAATCCAGCTTTN
  CTGCAGAGTGGNTCCCTGTGACAGTATTTGCCTTACTTTCCCCAGAGGGTCTGCTGATCA
  TCCGAGCTCCCCAGNTCCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014365 unedited
  GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTAAGAACGT
  CAGCTCCTTTATTATTATTATTATTAATTATTTACTGGTATTTACCCCTAAACAACAGCA
  TAACTCAAATAATAATGACACACACGTCCCGCCCATATACACAATACCACTAGCCTATCT
  GTCAGGCTATCTGGCCTTTGCTTGGTTCCTGATGGAGCTGTCTGGAGACAGCTCCCTCTG
  TAAAAATCCTGACTTAAACACAGGGGACAGAAGAAAGGGGGGACCTAGGCCAGATCATAA
  ACTGACAGGCTCCCAGCGTCCTTAGGGAGTGCTAATGTGGAAACTTTGAGAACGTGCTGG
  ACACATCTGGGCAGAGGGCAGAAGGCACTGTTTGTTTTTATGTGGTTGATGGATAAATTC
  CATATGGGGATATAAGACCTGTATATCACCCATTTTAGGCCAGGCATGTGGAAGCACAGT
  GGGGTTAGCTACTGGGTTTCATTAACCAAACCATGCGGCATGGTTTGGGAGCCATGGGAA
  TCGGGAGCCCAGAGTAGGAACCTGGGTGATGGGGGAGGTCCCCAGAGAAAAGTAACCCTT
  GCATTCACTGCAAGTAAGATACAACGTGAAATGTTAAGTCCCCTTTTATGTGTTTTGCAA
  CTATAGAATGTGCAACATTTTCATCAAGATAGAAAAGGTAAAGAAAGCAAAACTAGTATT
  TTTGGCCTAACACAACCAAGCATGATTTGCCAATTGCGCTATCCTGTGGAGAAATCTACC
  ACTACACTATCCAAGGAATCTTGTGGTCAGTCCTCCACCCCGCACCCTCTAACATTTTAC
  TTGCACTAAACCGAGGTCACTAAAAGTATGGATCCTGGAATCAAAAAACCCCTAGGTTGG
  TGACCAAACCAGGATGGTCCAGTCCTGCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014365
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014365.2, NP_055180.1
• RefSeq Size: 2056 bp
• RefSeq ORF: 591 bp
• Locus ID: 26353
• UniProt ID: Q9UJY1
• Cytogenetics: 12q24.23
• Domains: HSP20
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]