RAB30 (NM_014488) Human Untagged Clone

CAT#: SC114984

RAB30 (untagged)-Human RAB30, member RAS oncogene family (RAB30)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114984 sequence for NM_014488 edited (data generated by NextGen Sequencing)
  ATGAGTATGGAAGATTATGATTTCCTGTTCAAAATTGTTTTAATTGGCAACGCTGGTGTG
  GGGAAGACGTGCCTCGTCCGAAGATTCACTCAGGGTCTTTTCCCCCCAGGTCAAGGAGCC
  ACAATTGGAGTTGATTTTATGATTAAGACAGTGGAGATTAATGGTGAAAAAGTAAAGCTA
  CAGATCTGGGACACAGCAGGTCAAGAGAGATTTCGGTCCATTACCCAGAGTTACTACCGA
  AGCGCCAATGCCTTGATCCTCACCTATGACATTACCTGTGAGGAATCCTTCCGTTGCCTT
  CCTGAGTGGCTGCGGGAGATAGAACAATATGCCAGCAACAAGGTCATCACTGTGTTAGTG
  GGCAACAAGATTGACCTGGCTGAAAGGAGAGAGGTTTCCCAGCAGCGAGCTGAAGAATTC
  TCAGAAGCTCAGGACATGTATTATCTGGAGACCTCAGCCAAGGAATCTGATAATGTGGAG
  AAACTCTTCCTTGACTTAGCATGCCGACTCATCAGTGAAGCCAGACAGAACACACTTGTG
  AACAATGTATCCTCACCCTTACCTGGAGAAGGGAAAAGCATCAGCTATTTGACTTGTTGT
  AATTTCAACTAA
  
  Clone variation with respect to NM_014488.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014488 unedited
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGCGCAGACCT
  TCCCTTGCGTGCGTGACTCATTCGCACATCCATTTCTGTTTATGCCTGGGGTGGGGAGAA
  AAATCCCAAACCAGCTGTGTAAATGAGTATGGAAGATTATGATTTCCTGTTCAAAATTGT
  TTTAATTGGCAACGCTGGTGTGGGGAAGACGTGCCTCGTCCGAAGATTCACTCAGGGTCT
  TTTCCCCCCAGGTCAAGGAGCCACAATTGGAGTTGATTTTATGATTAAGACAGTGGAGAT
  TAATGGTGAAAAAGTAAAGCTACAGATCTGGGACACAGCAGGTCAAGAGAGATTTCGGTC
  CATTACCCAGAGTTACTACCGAAGCGCCAATGCCTTGATCCTCACCTATGACATTACCTG
  TGAGGAATCCTTCCGTTGCCTTCCTGAGTGGCTGCGGGAGATAGAACAATATGCCAGCAA
  CAAGGTCATCACTGTGTTAGTGGGCAACAAGATTGACCTGGCTGAAAGGAGAGAGGTTTC
  CCAGCAGCGAGCTGAAGAATTCTCAGAAGCTCAGGACATGTATTATCTGGAGACCTCAGC
  CAAGGAATCTGATAATGTGGAGAAACTCTTCCTTGACTTAGCATGCCGACTCATCAGTGA
  AGCCAGACAGAACACACTTGTGAACAATGTATCCTCACCCTTACCTGGAGAAGGGAAAAG
  CATCAGCTATTTGACTTGTTGTAATTTCAACTAAAGGCTGANGCACGGAGAAAGAAAGGA
  AATCAGCACTGCCCTGATGGGCCATGAGATGCTGNNGGAGATCTGGCGATGACTGTGGCT
  CCCGCTCTCTGACCTTCTGACTNCTGTGGNCTNCTGAGCTTACAAGCATGGCAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014488
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014488.3, NP_055303.2
• RefSeq Size: 1619 bp
• RefSeq ORF: 612 bp
• Locus ID: 27314
• UniProt ID: Q15771
• Cytogenetics: 11q14.1
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.