FEM 1B (FEM1B) (NM_015322) Human Untagged Clone

SKU SC114716

FEM1B (untagged)-Human fem-1 homolog b (C. elegans) (FEM1B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FEM 1B
• Synonyms: F1A-ALPHA; F1AA; FEM1-beta
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114716 sequence for NM_015322 edited (data generated by NextGen Sequencing); ATGGAGGGCCTGGCTGGCTATGTATACAAGGCGGCCAGCGAGGGCAAGGTGCTGACTCTG; GCCGCCTTGCTTCTCAACCGGTCTGAAAGCGACATCCGCTATCTGCTTGGCTATGTCAGC; CAGCAGGGAGGGCAGCGCTCCACGCCCCTCATCATCGCAGCCCGCAATGGACACGCAAAG; GTGGTACGCTTGCTCTTAGAACATTACCGGGTGCAGACTCAGCAGACTGGCACCGTCCGC; TTCGACGGGTATGTCATTGATGGTGCCACTGCTCTTTGGTGTGCAGCTGGAGCAGGACAT; TTTGAAGTTGTTAAACTTCTAGTCAGCCATGGAGCCAACGTGAACCATACCACAGTAACT; AATTCAACCCCCCTGCGGGCAGCATGCTTTGATGGCAGACTGGACATTGTGAAATACTTG; GTTGAAAATAATGCCAACATCAGCATTGCCAACAAATATGACAACACCTGCCTAATGATT; GCGGCATATAAGGGACACACTGATGTGGTCAGATACCTTTTAGAACAACGTGCTGATCCC; AATGCCAAAGCACATTGTGGAGCCACAGCATTGCACTTTGCAGCTGAAGCTGGGCACATA; GATATTGTGAAAGAGCTGATAAAATGGCGTGCTGCTATAGTAGTGAATGGCCATGGGATG; ACGCCATTGAAAGTAGCTGCCGAAAGCTGTAAAGCTGATGTCGTAGAACTGTTACTCTCT; CATGCTGATTGCGACCGAAGAAGTCGGATTGAAGCTTTGGAACTCTTGGGTGCCTCCTTT; GCAAATGACCGTGAGAACTATGACATCATAAAGACATACCACTATCTATATTTAGCCATG; TTAGAGAGGTTCCAAGATGGTGATAACATTCTCGAAAAAGAGGTTCTTCCACCAATCCAT; GCTTATGGGAATAGAACTGAATGTAGAAATCCTCAGGAACTGGAGTCCATTCGGCAAGAC; AGAGATGCTCTTCATATGGAAGGCCTTATAGTTCGGGAACGGATTTTAGGTGCTGACAAT; ATTGATGTTTCTCATCCCATCATTTACAGAGGAGCTGTTTATGCGGATAATATGGAATTT; GAGCAGTGTATCAAGTTGTGGCTTCATGCCCTGCACCTCAGACAAAAAGGTAACAGGAAC; ACCCACAAGGATCTTCTTCGATTTGCTCAAGTTTTCTCACAAATGATACATTTGAATGAA; ACTGTGAAGGCCCCAGACATAGAATGTGTTTTGAGATGCAGTGTTTTGGAAATAGAACAA; AGTATGAACAGAGTGAAAAATATTTCAGATGCTGATGTCCACAATGCTATGGACAATTAT; GAATGTAATCTCTATACCTTTCTGTATTTAGTGTGCATCTCTACCAAAACACAGTGCAGC; GAAGAAGATCAGTGCAAAATTAACAAGCAGATCTACAACCTGATTCACCTTGATCCCAGA; ACTCGTGAAGGTTTCACCTTGCTGCATCTGGCTGTCAACTCCAATACTCCAGTTGATGAT; TTCCACACCAATGACGTCTGCAGCTTTCCAAATGCACTTGTCACAAAGCTCCTGCTGGAC; TGTTGTGCTGAGGTGAATGCCGTGGACAATGAGGGAAACAGTGCCCTTCATATTATCGTT; CAGTACAACAGGCCCATCAGTGATTTTTTGACCTTGCACTCCATCATCATTAGCCTAGTT; GAAGCCGGAGCTCACACTGACATGACGAATAAACAGAATAAGACTCCGCTAGACAAAAGT; ACAACTGGGGTATCTGAAATACTGCTTAAAACTCAAATGAAGATGAGTCTCAAGTGCCTG; GCTGCCCGAGCAGTTCGGGCTAATGACATTAACTACCAAGACCAGATCCCCAGAACTCTT; GAAGAGTTTGTTGGATTTCATTAA; ; Clone variation with respect to NM_015322.3; 1564 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015322 unedited; TCAGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCTGGGCCG; CACTGCTGCCTGGGCGCGGCGGCGGCGACGGCGCCCTGTTGAATGGGCTGTGAGGGCCCA; GGTTTAAAGCGCTGGCGAACGCGGCCTCCGGGGGCGCACGGCAGCTGCAGCGGTGGCGAC; CAAACGGGTGTTGGAGTTGGCGGCGGCCATGGAGGGCCTGGCTGGCTATGTATACAAGGC; GGCCAGCGAGGGCAAGGTGCTGACTCTGGCCGCCTTGCTTCTCAACCGGTCTGAAAGCGA; CATCCGCTATCTGCTTGGCTATGTCAGCCAGCAGGGAGGGCAGCGCTCCACGCCCCTCAT; CATCGCAGCCCGCAATGGACACGCAAAGGTGGTACGCTTGCTCTTAGAACATTACCGGGT; GCAGACTCAGCAGACTGGCACCGTCCGCTTCGACGGGTATGTCATTGATGGTGCCACTGC; TCTTTGGTGTGCAGCTGGAGCAGGACATTTTGAAGTTGTTAAACTTCTAGTCAGCCATGG; AGCCAACGTGAACCATACCACAGTAACTAATTCAACCCCCCTGCGGGCAGCATGCTTTGA; TGGCAGACTGGACATTGTGAAATACTTGGTTGAAAATAATGCCAACATCAGCATTGNCAA; CAAATATGACAACACCTGCCTAATGATTGCGGCATATAAGGGACACACTGATGTGGTCAG; ATACCTTTTAGAACAACGTGCTGATTCCAATGCCAAAGCACATTGTGGAGCCACAGCATT; GCACTTTGCAGCTGAAGCTGGCACATAGATATTGTGGAAGAGCTGATAAATGGCGTGCTG; CTATANTANTGAATGGGCATGGNATGACGCCATTGAAAGTACTGNNCGAAGCTGTAAAGC; TGTGTCGTAAAATGTTACTTTCTCAGCTGATTGCGACCGCANAAGNTCGATTGAAGCTTT; GACTCTTTGGGTGGCCTCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015322 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAGTTTTTTTCAAACA; TTTACTGAACACAAACACCATTTTTTCCTTTTACACAGCAAGATTGTTAGTTTCAAATTA; GCTGAGTTATTAGGACTGTCAATTGCCATGAAACTAAAAATGACTCTACATGTCAAACTC; AAATAGAAAATGTCATGCAATATATAAATCACTAATGGACCTCTGGTGCAGTCAGCAAGT; TTACTATGTTTATTAAAGTCACATAAATGGTTTGACAAAAGTGCACAATAGAATGACATC; ACCGGTTTGTTATAAATCACAGTGAATTACTAGAATACAACAGTGTAGTTCCTCTGCACG; GAGGATTGCAGGATGGGGTGAAAAGGACAGGGTCTTTTCCATCGATTAGGCCAGTCAGAT; TTTACTCCCCAACTGTGTACCTTTGAAAAGACTGTGAGGAAAGCTGAGTTTCTTTCCTCA; TCTTTTCTCCTCTACCAGTTACTAAGCAACAACAACAAAAAGCCATGCACAAATTTTACA; ACACCTGATAAAAAATAGATACAAAATCTTGTTCATTTCATCATTTGGCAAGCTTGCATC; TTCGCTCCTTCACTTTTCTGATTAGGCCATTCATCACATGACTCACCCTGAATAAAAGCT; GTAAATAATTTAGCAATACTTACCCCTATACACACATCAAGATACACTCCCCATAACCAT; CAAGTCACTGTCTCTTCCCTACACATTACACANAGGTACACACCACAAACCTAGAGACCA; CATTACTTTACAGTTTTAAAAATAATATATCTCCCTATCAAAGTGTAGTATTTATTTCGA; ATCCCCTATTGGGGGAGGCATGCTGGATAACCAGCCAGCTAAAGATTGGGCAGTCTGCAG; AATACATAAAAACCTGCATGTGATAACCAAGGATTTTCTACTTCAGAATACTTCCGACAA; GCTTGGCTTTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_015322
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015322.3, NP_056137.1
• RefSeq Size: 2583 bp
• RefSeq ORF: 1884 bp
• Locus ID: 10116
• UniProt ID: Q9UK73
• Cytogenetics: 15q23
• Domains: ANK
• Protein Families: Druggable Genome
• Summary: This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1. [provided by RefSeq, Aug 2013]