ELOVL2 (NM_017770) Human Untagged Clone

CAT#: SC113934

ELOVL2 (untagged)-Human ELOVL fatty acid elongase 2 (ELOVL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELOVL2
• Synonyms: SSC2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113934 sequence for NM_017770 edited (data generated by NextGen Sequencing)
  ATGGAACATCTAAAGGCCTTTGATGATGAAATCAATGCTTTTTTGGACAATATGTTTGGA
  CCGCGAGATTCTCGAGTCAGAGGGTGGTTCATGTTGGACTCTTACCTTCCTACCTTTTTT
  CTTACTGTCATGTATCTGCTCTCAATATGGCTGGGTAACAAGTATATGAAGAACAGACCT
  GCTCTTTCTCTCAGGGGTATCCTCACCTTGTATAATCTTGGAATCACACTTCTCTCCGCG
  TACATGCTGGCAGAGCTCATTCTCTCCACTTGGGAAGGAGGCTACAACTTACAGTGTCAA
  GATCTTACCAGCGCAGGGGAAGCTGACATCCGGGTAGCCAAGGTGCTTTGGTGGTACTAT
  TTCTCCAAATCAGTAGAGTTCCTGGACACAATTTTCTTCGTTTTGCGGAAAAAAACGAGT
  CAGATTACTTTTCTTCATGTATATCATCATGCTTCTATGTTTAACATCTGGTGGTGTGTC
  TTGAACTGGATACCTTGTGGACAAAGTTTCTTTGGACCAACACTGAACAGTTTTATCCAC
  ATTCTTATGTACTCCTACTATGGACTTTCTGTGTTTCCATCTATGCACAAGTATCTTTGG
  TGGAAGAAATATCTCACACAGGCTCAGCTGGTGCAGTTCGTGCTCACCATCACGCACACC
  ATGAGCGCCGTCGTGAAACCGTGTGGCTTCCCCTTCGGTTGTCTCATCTTCCAGTCATCT
  TATATGCTAACGTTAGTCATCCTCTTCTTAAATTTTTACGTTCAGACATACCGAAAAAAG
  CCAATGAAGAAAGATATGCAAGAGCCACCTGCAGGGAAAGAAGTGAAGAATGGTTTTTCC
  AAAGCCTACTTCACTGCAGCAAATGGAGTGATGAACAAGAAAGCACAATAA
  
  Clone variation with respect to NM_017770.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017770 unedited
  CTATAGGGCGGCCGCGATTCGGCACGAGGGCGCCGAGATAGCGCCGGGCAGAGGGACCCG
  GCTACCCTGGACAGCGCATCGCCGCCCGCCCGGGTCGCCGCGCCACAGCCGCTGCGGATC
  ATGGAACATCTAAAGGCCTTTGATGATGAAATCAATGCTTTTTTGGACAATATGTTTGGA
  CCGCGAGATTCTCGAGTCAGAGGGTGGTTCATGTTGGACTCTTACCTTCCTACCTTTTTT
  CTTACTGTCATGTATCTGCTCTCAATATGGCTGGGTAACAAGTATATGAAGAACAGACCT
  GCTCTTTCTCTCAGGGGTATCCTCACCTTGTATAATCTTGGAATCACACTTCTCTCCGCG
  TACATGCTGGCAGAGCTCATTCTCTCCACTTGGGAAGGAGGCTACAACTTACAGTGTCAA
  GATCTTACCAGCGCAGGGGAAGCTGACATCCGGGTAGCCAAGGTGCTTTGGTGGTACTAT
  TTCTCCAAATCAGTAGAGTTCCTGGACACAATTTTCTTCGTTTTGCGGAAAAAAACGAGT
  CAGATTACTTTTCTTCATGTATATCATCATGCTTCTATGTTTAACATCTGGTGGTGTGTC
  TTGAACTGGATACCTTGTGGACAAAGTTTCTTTGGACCAACACTGAACAGTTTTATCCAC
  ATTCTTATGTACTCCTACTATGGACTTTCTGTGTTTCCATCTATGCACAAGTATCTTTTG
  GTGGAAGAAATATCTCACACAGGCTCAGCTGGTGCAGTTCGTGCTCACCATCACGCACAC
  CATGAAGCGCCGTCGTGAAAACGTGTGGGCTCCCCTTTCGNNTGNCTCATCTTCCAGNCA
  TCTTATATGCTAACGTTAGNCATCCTCTTCTTAAATTTTACGTTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017770 unedited
  TACCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAATGTAAAAATCAATT
  TATTATACAACAATCATAGATAATGCTTTTTATCTACAAAGAAAAATGGCTTCTGCAGCC
  TCCCTGTCTACTCCATTCATGATACTATGTTCTTAAGATATAATTACTTTCAAAGGAAAA
  CAAAGCGATATCCATATTTTCCAAACAAGGAAGCCCCCAGACACATTTATGAACGATATG
  GAAATATTGGAAAGAACTCAAATGGACTCCTAGATACAAAAGGCTGTTCTGCCCATCACA
  GTAAACACTGTTTTGCCTTAAAATAAAAATAATAAAATATTTCTCAAATGCAGGGGTGAG
  GACTTTACCCCGTAACATGCCTAAGTGGTTCGATATATAATTTTGATGGCTTGACAATTG
  CTATGTTTAATTCCATTCAGTTAACATTCCATTTTGTTAGTTTAAAAAATTGGGGGAATT
  ATTAATAGATATGCTTTACAGTATTTAAGTATTTTCATTCCCATGGGTCAGAGGAAATTT
  GAGAGAAGACATTCTCCATTTTTTTTAATAGATTATAGAAATATTAATTTTTCAATAGTT
  TTAGAAAAATCACATTAAAATAAGCATTTTGGTTTTTTGAAGTTACAACACTTAACGACA
  GGTTAAAATGTTTACCTGATTTATTTTTTGGTGCTAAGATTTCATAACTATTTTTTTAAT
  TAGCCCCTTGATGGTTTAAGGCTANTAGTGTTTTATGAAGCATCTCTTGNATTGAAGACC
  AAATTGGNAATACACATCATGAATGAAACCTCTCTCTAGTCACTGGCATTTAGTTGAAGT
  ATAAGCTACCCACATTTTCACTTTCATCAGAGAGTTNCAAGCTGCCCCTGTACTAAGCTG
  CTTGACTTNGTCTGCTGAAGGAAGCTCCNTCCTTGCATACAGATATGAGCCCCAGNAAAC
  ATCCNGATGGCCGGTATGCTATGCACTGAGACCTTAGGCCTNACTGGCCGTAAGAATAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017770
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017770.2, NP_060240.2
• RefSeq Size: 4038 bp
• RefSeq ORF: 891 bp
• Locus ID: 54898
• UniProt ID: Q9NXB9
• Cytogenetics: 6p24.2
• Domains: ELO
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA), acting specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA. May participate in the production of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function]