PSPC1 (NM_018282) Human Untagged Clone

SKU SC113593

PSPC1 (untagged)-Human paraspeckle component 1 (PSPC1), transcript variant beta

Product Data

• Type: Human Untagged Clone
• Target Symbol: PSPC1
• Synonyms: PSP1, FLJ10955, DKFZp566B1447
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018282, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATGTTAAGAGGAAACCTGAAGCAAGTGCGCATTGAGAAAAACCCGGCCCGCCTTCGCGCCCTGGAGT; CCGCGGTGGGCGAGAGCGAGCCGGCGGCCGCGGCAGCCATGGCGCTCGCTCTTGCCGGGGAGCCGGCACC; GCCCGCGCCCGCGCCTCCAGAGGACCACCCGGACGAGGAGATGGGGTTCACTATCGACATCAAGAGTTTC; CTCAAGCCGGGCGAGAAGACGTACACGCAGCGCTGCCGCCTCTTCGTGGGAAATCTGCCCACCGACATCA; CGGAGGAGGACTTCAAGAGGCTCTTCGAACGCTATGGCGAGCCCAGCGAAGTCTTCATCAACCGGGACCG; TGGCTTCGGCTTCATCCGCTTGGAATCCAGAACCCTGGCTGAAATTGCAAAAGCAGAGCTGGACGGCACC; ATTCTCAAGAGCAGACCTCTACGGATTCGCTTCGCTACACATGGAGCAGCCTTGACTGTCAAGAACCTTT; CTCCAGTTGTTTCCAATGAGCTGCTAGAGCAAGCATTTTCTCAGTTTGGTCCAGTAGAGAAAGCTGTTGT; GGTTGTGGATGATCGCGGTAGAGCTACAGGAAAAGGTTTTGTAGAGTTTGCAGCAAAACCTCCTGCACGA; AAGGCTCTGGAAAGATGTGGTGATGGGGCATTCTTGCTAACAACGACCCCTCGTCCAGTCATTGTGGAAC; CCATGGAGCAGTTTGATGATGAAGATGGCTTGCCAGAGAAGCTGATGCAGAAAACTCAACAATATCATAA; GGAAAGAGAACAACCACCACGTTTTGCTCAACCTGGGACATTTGAATTTGAGTATGCATCTCGATGGAAG; GCTCTTGATGAAATGGAAAAGCAGCAGCGTGAGCAGGTTGATAGAAACATCAGAGAAGCCAAAGAGAAAC; TGGAGGCAGAAATGGAAGCAGCTAGGCATGAACACCAATTAATGCTAATGAGGCAAGATCTAATGAGGCG; TCAAGAAGAACTCAGACGCTTGGAAGAACTCAGAAACCAAGAGTTGCAAAAACGGAAGCAAATACAACTA; AGACATGAAGAGGAGCATCGGCGGCGTGAGGAAGAAATGATCCGACACAGAGAACAGGAGGAACTGAGGC; GACAGCAAGAGGGCTTTAAGCCAAACTACATGGAAAATGGTGATAAAAGAAAATGTGGCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018282 unedited; TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGCGGGCGTGAG; TTGGGGGAGGACGGGTTGCCGACTCGCCTACCTAGCGGTCTCTTGATTGTCGATATTTTG; TTGGCATAGGTTTATGTAGAGACGTATACATATATATAGACACACTGTCTATAAATCTAG; GCCTGTATCCGGTGTCCGAGGCGAACTCAGTAAGATGATGTTAAGAGGAAACCTGAAGCA; AGTGCGCATTGAGAAAAACCCGGCCCGCCTTCGCGCCCTGGAGTCCGCGGTGGGCGAGAG; CGAGCCGGCGGCCGCGGCAGCCATGGCGCTCGCTCTTGCCGGGGAGCCGGCACCGCCCGC; GCCCGCGCCTCCAGAGGACCACCCGGACGAGGAGATGGGGTTCACTATCGACATCAAGAG; TTTCCTCAAGCCGGGCGAGAAGACGTACACGCAGCGCTGCCGCCTCTTCGTGGGAAATCT; GCCCACCGACATCACGGAGGAGGACTTCAAGAGGCTCTTCGAACGCTATGGCGAGCCCAG; CGAAGTCTTCATCAACCGGGACCGTGGCTTCGGCTTCATCCGCTTGGAATCCAGAACCCT; GGCTGAAATTGCAAAAGCAGAGCTGGACGGCACCATTCTCAAGAGCAGACCTCTACGGAT; TCGCTTCGCTACACATGGAGCAGCCTTGACTGTCAAGAACCTTTCTCCAGTTGTTTCCAA; TGAGCTGCTAGAGCAGCATTNTCTCAGTTTGGTCCAGTAGAAAAGCTGNNTGTGGTTGTG; GATGATCGCNGTAAACTACGGGAAAGGTTTTGGTANATTTGCAGCANAACTNCTGCACGA; AGCTCTGGAAGAGGGGGGGATGGGGCATTCTGCTACACGACCCTCGTCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018282 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAGAATTCGAAATGT; TTAATATTTCATGCAGTTAACATGCTTCAAAGCTTTAAGTCTGAAGTTTTAGACTTATCC; CAAATCCTTGCTAAGAAGTCAAGACCACTATGGAATTCACACTGCTTTCTATAGACTTCC; ACTGACTTGCAGCTAATGGTCCTTACACAGCAAAGTTGGGATTTCTAGACGTCTCCTTCC; AGGTTCGGAAAAGCCTCACTAGGCAAGTGGCAAGACTGTGAAGACAATGAAGGTTCAGAA; TTCAGTCAAATGGAAACAAGATCAGGAAACAAGATCAAGGACTCATCTGTCCGCCTGGCA; CCACTTTAGTTTTGAAGGTGACGCCACCACGCATCCCCGCAGCACACACTCCTTCCTGTG; CCGACTCCCGCTATTTGCTTGCCTGAGCCAGTTCATGTTTCAGTAAGAACGATTTTTCTC; CCAACAGTGCCAGTTTTCTTATTCCCAATTTTGAGAGTTGACCCAAAATTGTGGGCTATG; AGGGATGGTTTTTAAAACTCCTCTAATGGTTTCCAGGCACAGAGCGTTGAAAAGGAGGGA; TTTCTTGGCTATTCTGAATTTGTGCATTGCTGCCATACTACTTCCCAACACGAACCAGCA; AGACCACCACACCAAGGAGTGGGTAACATCTTGATTTAATTTACAAATAAAAAGCCAAAA; CCCTCAATGCAAAGGAAAACTGCAGTTTACACTTATTCTGGAGATCATTTTTAACCACAG; AAAATGTGCCCCTGTAGCATGTTTTTAAATGGGCAGTGCTATGATTGCCCANACATCCAT; NNAACTGCTGTATGGAAAATTCATAATGAAGAAATNCATAATGAAGAAAGNATTGNNTTT; CTGGCTTGGTTCTNCCTAGGCACGAACAGGATATTNCCCACAAATGTTNTGACTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_018282
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018282.1, NP_060752.1
• RefSeq Size: 1769 bp
• RefSeq ORF: 1368 bp
• Locus ID: 55269
• Cytogenetics: 13q12.11
• Domains: RRM
• Protein Families: Transcription Factors
• Summary: This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified. [provided by RefSeq, Aug 2011]