TREM2 (NM_018965) Human Untagged Clone

CAT#: SC113273

TREM2 (untagged)-Human triggering receptor expressed on myeloid cells 2 (TREM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TREM2
• Synonyms: PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018965 edited
  GAATTCGGCACGAGGCTGGAGCCGCCCCCGGGACGTCAGTCCTGGAGGAGGCGAGGGTTG
  CTCCGGTTGCTAAGAAGACTATGAACAAGTCAGAGAACCTGCTGTTTGCTGGTTCATCAT
  TAGCATCACAAGTCCATGCTGCTGCCGTTAATGGAGATAAGGGTGCTCTACAGAGGCTCA
  TCGTAGGAAACTCTGCTCTTAAAGACAAAGAAGATCAGTTTGGGAGAACACCACTTATGT
  ATTGCGTGTTGGCTGACAGATTGGATTGTGCAGATGCTCTTCTGAAGGCAGGAGCAGATG
  TGAATAAAACTGACCATAGCCAGAGAACAGCCCTCCATCTTGCAGCCCAGAAGGGAAATT
  ATCGTTTCATGAAACTCTTACTTACACGCAGAGCAAACTGGATGCAAAAGGATCTGGAAG
  AGATGACTCCTTTGCACTTGACCACCCGGCACAGGAGCCCTAAGTGTTTGGCACTTCTGC
  TGAAGTTTATGGCACCAGGAGAAGTGGATACACAGGATAAAAACAAGCAAACAGCTCTGC
  ATTGGAGTGCCTACTACAATAACCCTGAGCATGTGAAGCTGCTCATCAAGCATGATTCTA
  ACATTGGGATTCCTGATGTTGAAGGCAAGATCCCACTTCACTGGGCAGCCAACCATAAAG
  ATCCAAGTGCTGTTCACACAGTGAGATGCATTCTGGATGCTGCTCCAACAGAGTCTTTAC
  TGAACTGGCAAGACTACGAGGGTCGAACTCCTCTTCACTTTGCAGTTGCTGATGGGAATG
  TGACCGTGGTTGATGTCTTGACCTCATATGAAAGCTGCAATATAACGTCTTATGATAACT
  TATTTCGAACCCCACTGCACTGGGCAGCTTTATTAGGCCATGCACXXXXXXXXXXXXXXX
  XXXXXXXXXXXXAAAAACAAAACAAAACCTTGACTGCCTATGAAGGAAGACTGTGTTCGG
  GGGAGCTGGCATAGCTAGTGCAGAGTTCAGATTTTCTGCTGATAATCTTTTACACCTTGG
  GAAAACTTTAATATCCGTACCTGAAGGCTGATTCACCTAAAAATGTGTTAACTGAAAGAA
  AATGTCAGAATGTTTCCTTTCTGCTCTTACACAGCATTGTTTTGTCAATCAACACAGCCT
  GCACTGAAAGGACCTGCATAGACTATGTCTGTGCAAAGTGCCTGAGTGTCTGCTTTCACC
  TCAGTCTGTACAGTTGGAAATGAGAATTCATAATTAACAGCAAAATCTAAGGAAAACTAC
  GGCTGCTGGGTTGGGATTTCTGCCAGCTAGCTGGTACTGGCTTCTTGTTTCAAGAGATGA
  ACCTAAATGAGATAGGAAGCCTGTCCCATAGCAGCCTTCCTCTCACTACTTTCCTGGCAT
  CTAATGCAACAAACTTATCACACACACACACACACACACACACACACACACACATCACGT
  CCCACTATTACTTCAAAATTAATCAGGTTTAAATTTTAGATCAGAATCATTGCCCACTGT
  TTTCATTTAGAAATTGTCCAAAAGACCATAGATACATTCTGGTGATTCCTTACATTTTAC
  AGTTCCTACGTTAGCCTCTCAAGGCCACAAGTTGCCGCCACGTCTTCACTGAATGACTTT
  CTCAGTGCTGAATACCAACAGCCGTATTACAGTATCAAGGATTCTGTCAGGTAGGGTTTA
  CAGACCAGACTGTTCATGCAGCAAGGTGCTAACTCAGGCGTCAACGTTCTACTTGTATTT
  TCTGACCAACTTGCAGAAGTGAGCAATCTTCTTAAAATGCCATTTCTGTTGGCAGGTAAG
  AATGAAACGGCACACATCCTAGCATTCTAAGAACACCTGGTXXXXXXXXXXXXXXXAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018965 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGACATGCCTGAT
  CCTCTCTTTTCTGCAGTTCAAGGGAAAGACGAGATCTTGCACAAGGCACTCTGCTTCTGC
  CCTTGGCTGGGGAAGGGTGGCATGGAGCCTCTCCGGCTGCTCATCTTACTCTTTGTCACA
  GAGCTGTCCGGAGCCCACAACACCACAGTGTTCCAGGGCGTGGCGGGCCAGTCCCTGCAG
  GTGTCTTGCCCCTATGACTCCATGAAGCACTGGGGGAGGCGCAAGGCCTGGTGCCGCCAG
  CTGGGAGAGAAGGGCCCATGCCAGCGTGTGGTCAGCACGCACAACTTGTGGCTGCTGTCC
  TTCCTGAGGAGGTGGAATGGGAGCACAGCCATCACAGACGATACCCTGGGTGGCACTCTC
  ACCATTACGCTGCGGAATCTACAACCCCATGATGCGGGTCTCTACCAGTGCCAGAGCCTC
  CATGGCAGTGAGGCTGACACCCTCAGGAAGGTCCTGGTGGAGGTGCTGGCAGACCCCCTG
  GATCACCGGGATGCTGGAGATCTCTGGTTCCCCGGGGAGTCTGAGAGCTTCGAGGATGCC
  CATGTGGAGCACAGNNTNTCCAGGAGCCTCTTGGAAGGAGAAATCCCCTTCCCACCCACT
  TCCATCCTTCTCCTCCTGGCCTGCATCTTTCTCATCAAGATTCTAGCAGCCAGCGCCCTC
  TGGGCTGCAGCCCTGCATGGACAGAAAGCCAGGACACATCCACCCAGTGACCTGGACTGT
  GGCCATGACCCCAAGTATCAGCTCCAAACTCTGCCANGGCTGAGAGACACGTGAAGGAAA
  AGATGGGAGGAAAAGCCCAGNAGAATTCCACCAGGGACANNCCAGCCTGCATACTTTGCA
  CTTGGCACCAGACCTTCTGTCTGCTCTGCAGAACTCTTGCCTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018965 unedited
  GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATATGACAGTCT
  TGGATTTATTTGTAAGTGTTTAAAATGTCCAATATTCAGAAGTTGTCAGGTGTTCTTACC
  ACCTCCCCACTCCCTCAACCAGTCCCTGCTTCCAGGGTCCAGGAGAAGCAGTGTTCAGGC
  AGAGTAGTCTCTTGCCAGAGCAGAACAAGGAGTCCTGGTGGCCAAGTGGCAAGTATGCAG
  GCTGGGCTGGTCCCTGGTGGGACTTCTCCTGGGCTTTTCCTCCCATCATCTTCCTTCACG
  TGTCTCTCAGCCCTGGCAGAGTTTGGAGCTGATACCCTGGGTCATGGCCACAGTCCAGTT
  CACTGGGTGGATGTGTCCCTGGCTTCTGTCCATGCCAGGCTGCAGCCCAGAGGGCGCTGG
  CTGCTAGAATCTTGATGAGAAAGATGCAGGCCAGGAGGAGAAGGATGGAAGTGGGTGGGA
  AGGGGATTTCTCCTTCCAAGAGGCTCCTGGAGATGCTGTGCTCCACATGGGCATCCTCGA
  AGCTCTCAGACTCCCCGGGGAACCAGAGATCTCCAGCATCCCGGTGATCCAGGGGGTCTG
  CCAGCACCTCCACCAGGACCTTCCTGAGGGTGTCAGCCTCACTGCCATGGAGGCTCTGGC
  ACTGGTAGAGACCCCGCATCATGGGGTTGTAGATTCCGCAGCGTAATGGTGAGAGTGCCA
  CCCCAGGTATCGTCTGTGATGGCTGTGCTCCCATTCCACCTCCTCAGAAGACAGCAGCCA
  CAAGTTGTGCGTGCTGACCACCCGCTGGCATGGGCCCCTTCTCTCCCACCTGCGGGAACC
  AGCCCTTGCGCCCCCCCCAGTGCTTTATGGNAGTCATAGGGCCAAGACACCTGCAAGAAC
  TTGGCCCCNCCCGCCTGAACACTGGGTAGTGGTGGCCTCCGACACTTCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018965
• Insert Size: 1140 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018965.1, NP_061838.1
• RefSeq Size: 1041 bp
• RefSeq ORF: 693 bp
• Locus ID: 54209
• UniProt ID: Q9NZC2
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).