PLSCR3 (NM_020360) Human Untagged Clone

CAT#: SC113138

PLSCR3 (untagged)-Human phospholipid scramblase 3 (PLSCR3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLSCR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020360, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGGCTACTTGCCCCCCAAAGGCTACGCCCCTTCGCCCCCACCTCCCTACCCTGTCACCCCTGGGT
  ACCCGGAGCCGGCGCTACATCCTGGGCCCGGGCAGGCGCCAGTGCCCGCCCAGGTACCTGCCCCAGCTCC
  CGGCTTCGCCCTCTTCCCCTCGCCTGGCCCCGTGGCCTTGGGGTCTGCTGCCCCCTTCTTGCCACTGCCA
  GGGGTGCCTTCTGGCCTCGAATTCCTGGTGCAGATTGATCAGATTTTGATTCACCAGAAGGCTGAGCGAG
  TGGAAACGTTCCTAGGCTGGGAGACCTGTAATCGGTATGAACTGCGCTCTGGGGCCGGGCAGCCCCTGGG
  TCAGGCGGCCGAGGAGAGCAACTGCTGCGCCCGTCTGTGCTGTGGCGCCCGCCGGCCGCTGCGTGTCCGC
  CTGGCCGACCCCGGGGACCGTGAGGTGCTGCGTTTGCTCCGCCCGCTGCACTGTGGCTGCAGCTGCTGCC
  CCTGTGGCCTCCAGGAGATGGAAGTACAGGCTCCACCAGGCACCACCATTGGCCACGTGCTACAGACCTG
  GCATCCCTTCCTCCCCAAGTTCTCCATCCAGGATGCCGATCGCCAGACAGTCTTGCGAGTGGTGGGGCCC
  TGCTGGACCTGTGGCTGTGGCACAGACACCAACTTTGAGGTGAAGACTCGGGATGAATCCCGCAGTGTGG
  GCCGCATCAGCAAGCAGTGGGGGGGCCTGGTCCGAGAAGCCCTCACAGATGCAGATGACTTTGGCCTACA
  GTTCCCGCTGGACCTGGATGTGAGGGTGAAGGCTGTGCTGCTGGGAGCCACATTCCTCATTGACTACATG
  TTCTTTGAGAAGCGAGGAGGCGCTGGGCCCTCTGCCGTCACCAGTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020360 unedited
  GGGGTCGCAATTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGGTGCTAG
  GCACCCGGGCTCTTCTGGGGGCTCCAGAACTAAGCCACCCAGACACCATCATCTCGAAAA
  CCCCAGCCCTTCTCCCATGGCAGGTAAGTGCGCGCAAGTCTGGGGGTCTCCGTTATCCCC
  CCCACGGACTTGGGAGCCGGGTTTGAACACATCCTCCCGGAATGTGCTCGGACTGGCTGG
  GTGGGGCCCCTCCCTTCCTTCCGGGGAACCTCCACCCTGCTCCCCTGCCCCATCTTTCTC
  TGCCTCTTGCCACCACGTTTCCCCCGAAGCCTCGCGGCCCATGGGCTGAGGAATAAGGGG
  ATGAGGCCTTGAGTCCCTTTCTAATCTATCCTGCTCCCACCGCTTTACAGGCTACTTGCC
  CCCCAAAGGCTACGCCCCTTCGCCCCCACCTCCCTACCCTGTCACCCCTGGGTACCCGGA
  GCCGGCGCTACATCCTGGGCCCGGGCAGGCGCCAGTGCCCGCCCAGGTACCTGCCCCAGC
  TCCCGGCTTCGCCCTCTTCCCCTCGCCTGGCCCCGTGGCCTTGGGGTCTGCTGCCCCCTT
  CTTGCCACTGCCAGGGGTGCCTTCTGGCCTCGAATTCCTGGTGCAGATTGATCAGATTTT
  GATTCACCAGAAAGCTGAGCGAGTGGAAAGTAAGTAAAAGGAGTGGGAGGTTGGACCTCA
  TGATGGTGGCCCGGATGGTGGGCTCCTGNGCGCGCGAAGCTCTACGAGTCTCCCCGTGTC
  ACCNTCGGCAGCCGTTCTAAGCTGGGAGACCTGTTATCGGTATGAACTGCGCTCTGGGGC
  CGGGCAGCCCCTGGGTCAAGCGGCCCGAAGAGACAACTGCTGCGCCCGTCTGTGCTGTGG
  CGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020360 unedited
  GGCTTCTTGNACCCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTGTCCAAA
  AGTTACAAAGTTTATATGAATGTAACATATAAAGATGCAGCCTTTTTCCTAGGGCAAGGG
  AAGGAAGGGCAGTTACATAAGGCACAGGAATCGCAGCAAGCACCAGCTGAAGGCAGGGCC
  CTAGTGGGAGAGGAAGTTGGCACTGGGGTCCCCCTGCAGCTGTTGATGGGGAGGTAGAGA
  CCCCCTCAAAGTGCCATGGTCTGAGGCTGAACTAATTGGCCAAGCAGATGCTAGGCAGCG
  GCGGGGGGGCGGTGGCTATATCCACCGTCTTTGGTGCAGCTTGCCCTTCCACACCCCAGC
  CCTCTTGTAAAACCCCAGAGTCCTGGGTTCCAGAGGCCTCCTTTGGAGCAGAGGCCNCTG
  GAAGGGGGTGGGAGAGAGTGCATGGGGAAAGTGTGAAAGCTGATATGCCTGTGTGCCGAG
  GGACTGCTGGGACAGTGGTGGGAGGTANCANGAGAGCGGCTCTCATACATACCCCTCCTT
  GGGAACCACAGGGGCAGGCGGCCANGGAGTANGGTNGGGGATGGGGGCCCCCCTTCTGTC
  CCCTGCAGTGTACATGGAGGAAAGGGGCTGCCCANAGGAGGGGCCAGGGCANGTGACCAT
  CTGGAGTTCTGGGTCNAGTGATGGTCTCCTCACACAATGGTGGNNCTCTACTGGTGACGG
  CAGAGGGCCCANCGCCTNCTCGCTTCTCAAAGACATGTAGTCATGAGGAATGTGGGTCCC
  ACAGACAGGCTTTACCTTACATTCAGGTCCACGGGACTGTAGGCAAAGCATCTGCTTTGG
  TGAGGCTTTTTGGGACAAGCCCCCCACTGTTTGTTGTGCCGCCCAATGGGGGATATCCCC
  GGCCTACCTAAAAAGGGGGGGCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020360
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020360.2, NP_065093.2
• RefSeq Size: 1746 bp
• RefSeq ORF: 888 bp
• Locus ID: 57048
• UniProt ID: Q9NRY6
• Cytogenetics: 17p13.1
• Domains: Scramblase
• Gene Summary: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.