Caspase 1 (CASP1) (NM_033295) Human Untagged Clone

CAT#: SC112763

CASP1 (untagged)-Human caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) (CASP1), transcript variant epsilon

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASP1
• Synonyms: ICE; IL1BC; P45
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033295, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACAAGGTCCTGAAGGAGAAGAGAAAGCTGTTTATCCGTTCCATGGGTGAAGATAATGTTTCTT
  GGAGACATCCCACAATGGGCTCTGTTTTTATTGGAAGACTCATTGAACATATGCAAGAATATGCCTGTTC
  CTGTGATGTGGAGGAAATTTTCCGCAAGGTTCGATTTTCATTTGAGCAGCCAGATGGTAGAGCGCAGATG
  CCCACCACTGAAAGAGTGACTTTGACAAGATGTTTCTACCTCTTCCCAGGACATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033295 unedited
  GATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAGTAGGAGTTT
  CTGGAAACCTATCTTTACCAACTACAGAAGAGTTTGAGGATGATGCTATTAAGAAAGCCC
  ACATAGAGAAGGATTTTATCGCTTTCTGCTCTTCCACACCAGATAATGTTTCTTGGAGAC
  ATCCCACAATGGGCTCTGTTTTTATTGGAAGACTCATTGAACATATGCAAGAATATGCCT
  GTTCCTGTGATGTGGAGGAAATTTTCCGCAAGGTTCGATTTTCATTTGAGCAGCCAGATG
  GTAGAGCGCAGATGCCCACCACTGAAAGAGTGACTTTGACAAGATGTTTCTACCTCTTCC
  CAGGACATTAAAATAAGGAAACTGTATGAATGTCTGTGGGCAGGTACATGTGTATGGTCG
  GGAGTGTGGGAAGGTTGAGGAAAGGGTACTGAAAGTCCATTTGAGTCAAGAACTCTAGGT
  TTACAGGCTGAGAATCCTTAATCCAAAAATTTGAATTTTGAAATGCTCTAAAATCCAACA
  CTGTGTGAGCGCCCACATGATATTCAAAGGAAATGTTTATTGAAACATTTCAAATTATAG
  GTTTTTGGATTAGGGATGCTAAACCAGTAAGTATACAGCTGAATTCCAATATACAAAAAT
  ATCTGAAATTTGAAATACTTCTGGTAGCATGCATTNTGGATAAGGGATAATCAAGCCATA
  TACANGAAATACTGAAGTAATGTCTTTCTTCTGGTCAGTGCAGAGCACGTTGCTCTTCTC
  CCAAGTTTTTCATTTAGACCCCTTTTGAGATCATCTGATATTGGCTAGAAGTGCCGTAGA
  CTACAACCCTCACTTTTGATGTTTCTCTCCTTCCCTCAGCAGGNTATATTAAACATAGCA
  TGTGGNNCTTCCTTTTAAAATGTGTATGNTCCCCATGGTGGNNNATACTTATACTNGCAT
  GTCTTTACAAAATCCTTTNCTTGTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033295
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033295.1, NP_150637.1
• RefSeq Size: 443 bp
• RefSeq ORF: 267 bp
• Locus ID: 834
• UniProt ID: P29466
• Cytogenetics: 11q22.3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (epsilon) has multiple differences in the coding region but the translation remains in frame, compared to variant alpha. Variant epsilon encodes a protein lacking an internal segment, compared to isoform alpha. Isoform epsilon lacks apoptosis-inducing activity. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.