TLR2 (NM_003264) Human Untagged Clone

CAT#: SC112722

TLR2 (untagged)-Human toll-like receptor 2 (TLR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLR2
• Synonyms: CD282; TIL4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003264 edited
  GAATTCGGCACGAGGCGGAGGCAGCGAGAAAGCGCAGCCAGGCGGCTGCTCGGCGTTCTC
  TCAGGTGACTGCTCGGAGTTCTCCCAGTGTTTGGTGTTGCAAGCAGGATCCAAAGGAGAC
  CTATAGTGACTCCCAGGAGCTCTTAGTGACCAAGTGAAGGTACCTGTGGGGCTCATTGTG
  CCCATTGCTCTTTCACTGCTTTCAACTGGTAGTTGTGGGTTGAAGCACTGGACAATGCCA
  CATACTTTGTGGATGGTGTGGGTCTTGGGGGTCATCATCAGCCTCTCCAAGGAAGAATCC
  TCCAATCAGGCTTCTCTGTCTTGTGACCGCAATGGTATCTGCAAGGGCAGCTCAGGATCT
  TTAAACTCCATTCCCTCAGGGCTCACAGAAGCTGTAAAAAGCCTTGACCTGTCCAACAAC
  AGGATCACCTACATTAGCAACAGTGACCTACAGAGGTGTGTGAACCTCCAGGCTCTGGTG
  CTGACATCCAATGGAATTAACACAATAGAGGAAGATTCTTTTTCTTCCCTGGGCAGTCTT
  GAACATTTAGACTTATCCTATAATTACTTATCTAATTTATCGTCTTCCTGGTTCAAGCCC
  CTTTCTTCTTTAACATTCTTAAACTTACTGGGAAATCCTTACAAAACCCTAGGGGAAACA
  TCTCTTTTTTCTCATCTCACAAAATTGCAAATCCTGAGAGTGGGAAATATGGACACCTTC
  ACTAAGATTCAAAGAAAAGATTTTGCTGGACTTACCTTCCTTGAGGAACTTGAGATTGAT
  GCTTCAGATCTACAGAGCTATGAGCCAAAAAGTTTGAAGTCAATTCAGAATGTAAGTCAT
  CTGATCCTTCATATGAAGCAGCATATTTTACTGCTGGAGATTTTTGTAGATGTTACAAGT
  TCCGTGGAATGTTTGGAACTGCGAGATACTGATTTGGACACTTTCCATTTTTCAGAACTA
  TCCACTGGTGAAACAAATTCATTGATTAAAAAGTTTACATTTAGAAATGTGAAAATCACC
  GATGAAAGTTTGTTTCAGGTTATGAAACTTTTGAATCAGATTTCTGGATTGTTAGAATTA
  GAGTTTGATGACTGTACCCTTAATGGAGTTGGTAATTTTAGAGCATCTGATAATGACAGA
  GTTATAGATCCAGGTAAAGTGGAAACGTTAACAATCCGGAGGCTGCATATTCCAAGGTTT
  TACTTATTTTATGATCTGAGCACTTTATATTCACTTACAGAAAGAGTTAAAAGAATCACA
  GTAGAAAACAGTAAAGTTTTTCTGGTTCCTTGTTTACTTTCACAACATTTAAAATCATTA
  GAATACTTGGATCTCAGTGAAAATTTGATGGTTGAAGAATACTTGAAAAATTCAGCCTGT
  GAGGATGCCTGGCCCTCTCTACAAACTTTAATTTTAAGGCAAAATCATTTGGCATCATTG
  GAAAAAACCGGAGAGACTTTGCTCACTCTGAAAAACTTGACTAACATTGATATCAGTAAG
  AATAGTTTTCATTCTATGCCTGAAACTTGTCAGTGGCCAGAAAAGATGAAATATTTGAAC
  TTATCCAGCACACGAATACACAGTGTAACAGGCTGCATTCCCAAGACACTGGAAATTTTA
  GATGTTAGCAACAACAATCTCAATTTATTTTCTTTGAATTTGCCGCAACTCAAAGAACTT
  TATATTTCCAGAAATAAGTTGATGACTCTACCAGATGCCTCCCTCTTACCCATGTTACTA
  GTATTGAAAATCAGTAGGAATGCAATAACTACGTTTTCTAAGGAGCAACTTGACTCATTT
  CACACACTGAAGACTTTGGAAGCTGGTGGCAATAACTTCATTTGCTCCTGTGAATTCCTC
  TCCTTCACTCAGGAGCAGCAAGCACTGGCCAAAGTCTTGATTGATTGGCCAGCAAATTAC
  CTGTGTGACTCTCCATCCCATGTGCGTGGCCAGCAGGTTCAGGATGTCCGCCTCTCGGTG
  TCGGAATGTCACAGGACAGCACTGGTGTCTGGCATGTGCTGTGCTCTGTTCCTGCTGATC
  CTGCTCACGGGGGTCCTGTGCCACCGTTTCCATGGCCTGTGGTATATGAAAATGATGTGG
  GCCTGGCTCCAGGCCAAAAGGAAGCCCAGGAAAGCTCCCAGCAGGAACATCTGCTATGAT
  GCATTTGTTTCTTACAGTGAGCGGGATGCCTACTGGGTGGAGAACCTTATGGTCCAGGAG
  CTGGAGAACTTCAATCCCCCCTTCAAGTTGTGTCTTCATAAGCGGGACTTCATTCCTGGC
  AAGTGGATCATTGACAATATCATTGACTCCATTGAAAAGAGCCACAAAACTGTCTTTGTG
  CTTTCTGAAAACTTTGTGAAGAGTGAGTGGTGCAAGTATGAACTGGACTTCTCCCATTTC
  CGTCTTTTTGATGAGAACAATGATGCTGCCATTCTCATTCTTCTGGAGCCCATTGAGAAA
  AAAGCCATTCCCCAGCGCTTCTGCAAGCTGCGGAAGATAATGAACACCAAGACCTACCTG
  GAGTGGCCCATGGACGAGGCTCAGCGGGAAGGATTTTGGGTAAATCTGAGAGCTGCGATA
  AAGTCCTAGGTTCCCATATTTAAGACCAGTCTTTGTCTAGTTGGGATCTTTATGTCACTA
  GTTATAGTTAAGTTCATTCAGACATAATTATATAAAAACTACGTGGATGTACCGTCATTT
  GAGGACTTGCTTACTAAAACTACAAAACTTCAAATTTTGTCTGGGGTGCTGTTTTATAAA
  CATATGCCAGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003264 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGAGGCAGCGAGAAAGCGC
  AGCCAGGCGGCTGCTCGGCGTTCTCTCAGGTGACTGCTCGGAGTTCTCCCAGTGTTTGGT
  GTTGCAAGCAGGATCCAAAGGAGACCTATAGTGACTCCCAGGAGCTCTTAGTGACCAAGT
  GAAGGTACCTGTGGGGCTCATTGTGCCCATTGCTCTTTCACTGCTTTCAACTGGTAGTTG
  TGGGTTGAAGCACTGGACAATGCCACATACTTTGTGGATGGTGTGGGTCTTGGGGGTCAT
  CATCAGCCTCTCCAAGGAAGAATCCTCCAATCAGGCTTCTCTGTCTTGTGACCGCAATGG
  TATCTGCAAGGGCAGCTCAGGATCTTTAAACTCCATTCCCTCAGGGCTCACAGAAGCTGT
  AAAAAGCCTTGACCTGTCCAACAACAGGATCACCTACATTAGCAACAGTGACCTACAGAG
  GTGTGTGAACCTCCAGGCTCTGGTGCTGACATCCAATGGAATTAACACAATAGAGGAAGA
  TTCTTTTTCTTCCCTGGGCAGTCTTGAACATTTAGACTTATCCTATAATTACTTATCTAA
  TTTATCGTCTTCCTGGTTCAAGCCCCTTTCTTCTTTAACATTCTTAAACTTACTGGGAAA
  TCCTTANCAAACCCTAGGGGAAACATCTCTTTTTTCTCATCTCACAAAATTGCAAATCCT
  GAGAGTGGGAAATATGGACACCTTCACTAAGATTCAAAGAAAAGAATTTGCTGGACTTNA
  CCTTCCTTGAGAAACTGAGATTGATGCTTCAGATCTACAGAGCTATGAGCCANAANGGNT
  TGAAGTCAATTAGAANTGTAAGTCATCTGATCCTTATATGAAGCAGCATATTTACTGCTG
  GNAGATTNGGTAAAGGTACCAGTTCGGGGAATGTTTTGGAACTGGAGAACTGATTTGCAC
  CTTTCATTTTCAGACTACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003264 unedited
  GCCGCTTCTAAAGTCGAGNTCTTTTTTTTTTTTTTTTTTTTTTTTTTAAATCTGGCATAT
  GTTTATAAAACAGCACCCCAGACAAAATTTGAAGTTTTGTAGTTTTAGTAAGCAAGTCCT
  CAAATGACGGCACATCCACGCAGTTTTTATATAATTATGCCTGAATGAACTTAACTATAA
  CTAGTGACATAAAGATCCCAACTAGACAAAGACTGGCCTTAAATATGGGAACCTAGGACT
  TTATCGCAGCTCTCAGATTTACCCAAAATACTTCCCGCTGAGCCTCGCCCATGGGCCACT
  CCAGGTAGGTCTTGGTGTTCATTATCTTCCGCAGCTTGCAAAAGCGCTGGGGAATGGCTT
  TTTTCTCAATGGGCTCCAGAAGAATGAGAATGGCAGCTTCATTGTTCTCATCAAAAATAC
  GGAAATGGGAGAATTTCAGCTCATACTTGCACCACTCACTCTTAACAAAGTTTTCATAAA
  GCCACAAAGACAGCTTTGGGGCTCTTTTCAATGGAGTCAATGATATTGTCAATGATCCAC
  TTGCCAGGAATGAAGCCCCGCTTATGAAGACACAACTTGAAGGGGGGATTGAAGTTCTCC
  AACTCCTGGACCATAAGGGCTCTCTCCCCAGCTAGCCTACCCGTTCACTGTAAGAAACAA
  ATGCCTTATACCAAATGTCCCTGCTGGGACCTTTCCTGGGCCTTCTTTTGGCCTGTAGCC
  AGCCCCCCTTATTTTCATTTCCCAAGGCCTGGAATCGGTGGCCACGACCCCCCGACCGGA
  TTACTTGAACAATCCAACCCTGCCAAAACCAGGCTGTCTGGAATTCCACCCGAAAGGGGC
  TTCTAACTGTGGCCCCCCTGGGAGGAATCCACAGTATTGCGGCCAAAATAAAATTTGGCC
  ATCTGTGTCCTGTGAAGAAGAATCACGACCACGTATTCGCCCACTCTCAACTAAGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003264
• Insert Size: 2810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003264.3, NP_003255.2
• RefSeq Size: 3417 bp
• RefSeq ORF: 2355 bp
• Locus ID: 7097
• UniProt ID: O60603
• Cytogenetics: 4q31.3
• Domains: TIR, LRRCT, LRR, LRR_TYP, LRR_BAC, LRR_PS
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This protein is a cell-surface protein that can form heterodimers with other TLR family members to recognize conserved molecules derived from microorganisms known as pathogen-associated molecular patterns (PAMPs). Activation of TLRs by PAMPs leads to an up-regulation of signaling pathways to modulate the host's inflammatory response. This gene is also thought to promote apoptosis in response to bacterial lipoproteins. This gene has been implicated in the pathogenesis of several autoimmune diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-8 all encode the same protein.