Cryptochrome I (CRY1) (NM_004075) Human Untagged Clone

CAT#: SC112662

CRY1 (untagged)-Human cryptochrome 1 (photolyase-like) (CRY1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cryptochrome I
• Synonyms: DSPD; PHLL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112662 sequence for NM_004075 edited (data generated by NextGen Sequencing)
  ATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGGCTCCGGCTCCACGACAACCCCGCC
  CTGAAGGNGTGCATTCAGGGCGCCGACACCATCCGCTGCGTCTACATCCTGGACCCCTGG
  TTCGCCGGCTCCTCCAATGTGGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAG
  GATCTTGATGCCAATCTACGAAAATTAAACTCCCGTCTGTTTGTGATTCGTGGACAACCA
  GCAGATGTGTTTCCCAGGCTTTTCAAGGAATGGAACATTACTAAACTTTCAATTGAGTAT
  GATTCTGAGCCCTTTGGAAAGGAACGAGACGCAGCTATTAAGAAACTGGCAACTGAAGCT
  GGAGTAGAAGTCATTGTAAGAATTTCACATACATTATATGACCTAGACAAGATCATAGAA
  CTCAATGGTGGACAACCGCCTCTAACTTATAAAAGATTCCAGACTCTCATCAGCAAAATG
  GAACCACTAGAGATACCAGTAGAGACAATTACTTCAGAAGTGATAGAAAAGTGCACAACT
  CCTCTGTCTGATGACCATGATGAGAAATATGGAGTCCCTTCACTGGAAGAGCTAGGTTTT
  GATACAGATGGCTTATCCTCTGCAGTGTGGCCAGGCGGAGAAACTGAAGCACTTACTCGT
  TTGGAAAGGCATTTGGAAAGAAAAGCTTGGGTGGCAAATTTTGAAAGACCTCGAATGAAT
  GCGAATTCTCTGCTTGCAAGCCCTACTGGACTTAGTCCTTATCTCCGATTTGGTTGTTTG
  TCATGTCGACTGTTTTACTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAGAACAGT
  TCCCCTCCCCTTTCCCTTTATGGGCAACTGTTATGGCGTGAATTTTTCTATACAGCAGCA
  ACAAATAATCCACGCTTTGATAAAATGGAAGGAAACCCTATCTGTGTTCAGATTCCTTGG
  GATAAAAATCCTGAGGCTTTAGCCAAATGGGCGGAAGGCCGGACAGGCTTTCCATGGATT
  GATGCCATCATGACACAGCTTCGTCAGGAGGGTTGGATTCATCATCTAGCCAGGCATGCA
  GTTGCTTGCTTCCTGACACGAGGGGACCTGTGGATTAGTTGGGAAGAAGGAATGAAGGTA
  TTTGAAGAATTATTGCTTGATGCAGATTGGAGCATAAATGCTGGAAGTTGGATGTGGCTG
  TCTTGTAGTTCCTTTTTTCAACAGTTTTTTCACTGCTATTGCCCTGTTGGTTTTGGTAGG
  AGAACAGATCCCAATGGAGACTATATCAGGCGTTATTTGCCTGTCCTAAGAGGCTTCCCT
  GCAAAATATATCTATGATCCCTGGAATGCACCAGAAGGTATCCAAAAGGTAGCCAAATGT
  TTGATAGGAGTTAATTATCCTAAACCAATGGTGAACCATGCTGAGGCAAGCCGTTTGAAT
  ATCGAAAGGATGAAACAGATCTATCAGCAGCTTTCACGATATAGAGGACTAGGTCTTCTG
  GCATCAGTACCTTCTAATCCTAATGGGAATGGAGGCTTCATGGGATATTCTGCAGAAAAT
  ATCCCAGGTTGTAGCAGCAGTGGAAGTTGCTCTCAAGGGAGTGGTATTTTACACTATGCT
  CATGGCGACAGTCAGCAAACTCACCTGTTGAAGCAAGGAAGAAGCTCCATGGGCACTGGT
  CTCAGTGGTGGGAAACGTCCTAGTCAGGAAGAGGACACACAGAGTATTGGTCCTAAAGTC
  CAGAGACAGAGCACTAATTAG
  
  Clone variation with respect to NM_004075.3
  68 a=>n;636 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004075 unedited
  ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTTTTTCCTGTTC
  CCCCTCTGCCGCCCTTTCCTCACGCCCCGGGTGAGGCAATTCTCTTGGAAGCGAAGGTGT
  CGGCTATGAGCCGGAGCCTCCTTCCTTGAATTTCTCCGTGGAGGACCCGCCGCGCCCCCC
  GGCATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGGCTCCGGCTCCACGACAACCCC
  GCCCTGAAGGAGTGCATTCAGGGCGCCGACACCATCCGCTGCGTCTACATCCTGGACCCC
  TGGTTCGCCGGCTCCTCCAATGTGGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTT
  GAGGATCTTGATGCCAATCTACGAAAATTAAACTCCCGTCTGTTTGTGATTCGTGGACAA
  CCAGCAGATGTGTTTCCCAGGCTTTTCAAGGAATGGAACATTACTAAACTTTCAATTGAG
  TATGATTCTGAGCCCTTTGGAAAGGAACGAGACGCAGCTATTAAGAAACTGGCAACTGAA
  GCTGGAGTAGAAGTCATTGTAAGAATTTCACATACATTATATGACCTAGACAAGATCATA
  GAACTCAATGGTGGACAACCGCCTCTAACTTATAAAAGATTCCAGACTCTCATCAGCAAA
  ATGGAACCACTAGAGATACCAGTAGAGACAATTACTTCAGAAGTGATAGAAAAGTGCACA
  ACTCCTCTGTCTGATGACCATGATGAGAATATGGGAGTCCCTTCACTGGAGAGCTANGTT
  NTGAACAGATGGCTTNATCTCTGCAGTGTGGCCCAGCGGAGAACTGNAGCACTTACTCGT
  TTGGAAAAGCATTTGAAAGAAAGCTCGGTGGCAATTTTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004075 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATATTTGCATATACTTTAATAACAA
  GTTCATTTTAGTAATAAATGAACAAAGAAACAGAGACAGAGAACTTAACACATGATACTT
  CCACAAGTTTGGGTCTAAAAAGGTTTACATCAAAGTTTAAAACATATACTGTCTGTGTTA
  ACAGAGGCTACAATACCAATTTGGATTACGCACATTATCTAAAACATTATTATCAAAGAG
  TGCAAAGTTAGATCAAACAACATCAAGAAAATTCTGTCCTAAAATTTTAACGTCTTTTAA
  GAAAGTCTAAAAACCTCCTTTTTTCCCACTGACTTCAAAACTTTGAGTTTTATATCAAAA
  TATCAATTTGTCATTTTAACTTTACCATACATGAAAAATTATCAAAAATATATCGATGGG
  TCTATACTAATTGTGACTGTTTATATTTACATTAAGCAAATTCTCTTGCCAAGTTCTTTA
  TATACAAATTACCAATTGTACTCTGTGAAATTCATTAAAAAGTTAAAAACCACAGAAATG
  TCATTAGAAACAACATATTTCAATATGTAACTGCTTTCCATCAATGAAGAATATTTTTAA
  ATAACTTAATTGAAAAGTATTGAACTCCCCACCAATTTCAGCTGCAACAGTATTCCTCCT
  GAATGTTTTCTAATTAGTGCTCTGTCTCTGGACTTTANGACCAATACTCTGTGTGTCCTC
  TTCCTGACTAGGACGTTTCCCACCACTGAGACCAGTGCCCATGGAGCTTCTTNCTTGCTT
  CACANNGTGAGTTGCTGACTGTCCCATGAGCATAGTGAAAAACCACTCCCTTGAGAGCAC
  TTCCACTGCTGCTACACCTGGGATATTTCTGCAGATATCCCATGAGCCTCATTCCATTAG
  ATANAGGTACTGAGCCAAAGACTATCCTTTATTCGGAACTGTGATGATTGTTATCTTTCA
  TTAACGCTGCTACATGTACATGGTAGAAATACTCTAAACTTGCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004075
• Insert Size: 2590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004075.2, NP_004066.1
• RefSeq Size: 2999 bp
• RefSeq ORF: 1761 bp
• Locus ID: 1407
• UniProt ID: Q16526
• Cytogenetics: 12q23.3
• Domains: FAD_binding_7, DNA_photolyase
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]