RPS18 (NM_022551) Human Untagged Clone

CAT#: SC112508

RPS18 (untagged)-Human ribosomal protein S18 (RPS18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS18
• Synonyms: D6S218E; HKE3; KE-3; KE3; S18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112508 sequence for NM_022551 edited (data generated by NextGen Sequencing)
  ATGTCTCTAGTGATCCCTGAAAAGTTCCAGCATATTTTGCGAGTACTCAACACCAACATC
  GATGGGCGGCGGAAAATAGCCTTTGCCATCACTGCCATTAAGGGTGTGGGCCGAAGATAT
  GCTCATGTGGTGTTGAGGAAAGCAGACATTGACCTCACCAAGAGGGCGGGAGAACTCACT
  GAGGATGAGGTGGAACGTGTGATCACCATTATGCAGAATCCACGCCAGTACAAGATCCCA
  GACTGGTTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGTCCTAGCC
  AATGGTCTGGACAACAAGCTCCGTGAAGACCTGGAGCGACTGAAGAAGATTCGGGCCCAT
  AGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGC
  CGCCGTGGCCGCACCGTGGGTGTGTCCAAGAAGAAATAA
  
  Clone variation with respect to NM_022551.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022551 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCGCTTGTGCTG
  CAGCCATGTCTCTAGNNTGATCCCTGAAAAGTTCCAGCATATTTTGCGAGTACTCAACAC
  CAACATCGATGGGCGGCGGAAAATAGCCTTTGCCATCACTGCCATTAAGGGTGTGGGCCG
  AAGATATGCTCATGTGGTGTTGAGGAAAGCAGACATTGACCTCACCAAGAGGGCGGGAGA
  ACTCACTGAGGATGAGGTGGAACGTGTGATCACCATTATGCAGAATCCACGCCAGTACAA
  GATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGT
  CCTAGCCAATGGTCTGGACAACAAGCTCCGTGAAGACCTGGAGCGACTGAAGAAGATTCG
  GGCCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGAC
  CACTGGCCGCCGTGGCCGCACCGTGGGTGTGTCCAAGAAGAAATAAGTCTGTAGGCCTTG
  TCTGTTAATAAATAGTTTATATACCTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC
  GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC
  AGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAAAAAA
  TTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATATGGTGAGNNGGGGNGG
  GGGGNTNNNNNANNACAGAGGGCCACANNTNGGAAAACACACCTTGAGGCCTCCGGGGGG
  TTTTTTGGGGACCAACCTGGGAAGGCAGGGGCACATTCTGGGCTACTGGCATCTCGCCTT
  CTGGGGTCAGCGGATCTCTGCTACTCCCCCAGAGGGGGGGGAATACCAGCTGCCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022551 unedited
  NTTAGCTATGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGGTA
  TATAAACTATTTATTAACAGACAAGGCCTACAGACTTATTTCTTCTTGGACACACCCACG
  GTGCGGCCACGGCGGCCAGTGGTCTTGGTGTGCTGGCCTCGGACACGAAGGCCCCAGAAG
  TGACGCAGCCCTCTATGGGCCCGAATCTTCTTCAGTCGCTCCAGGTCTTCACGGAGCTTG
  TTGTCCAGACCATTGGCTAGGACCTGGCTGTATTTTCCATCCTTTACATCCTTCTGTCTG
  TTCAAGAACCAGTCTGGGATCTTGTACTGGCGTGGATTCTGCATAATGGTGATCACACGT
  TCCACCTCATCCTCAGTGAGTTCTCCCGCCCTCTTGGTGAGGTCAATGTCTGCTTTCCTC
  AACACCACATGAGCATATCTTCGGCCCACACCCTTAATGGCAGTGATGGCAAAGGCTATT
  TTCCGCCGCCCATCGATGTTGGTGTTGAGTACTCGCAAAATATGCTGGAACTTTTCAGGG
  ATCACTAGAGACATGGCTGCAGCACAAGCGGCGGCCCTCGTGCCGAATTCGCGGCCGCCC
  TATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGAC
  CTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGNGCGGNGTTATTACGACATTT
  TGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGGTGGA
  GACTTGGAAATCCCCGTGAGTCANACCGCTATCCACGCCATTGGTGTACTGCCAAAACGC
  ATACCATGTAATAGCGATGACTATACGTANATGTACTGGCCAGTAGAAAGCCCGTAGGTC
  ATGTACTGGGCATATGCCCAGCGCCATTTACCGCATTGACGCATAGGGGGCGACTNG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022551
• Insert Size: 580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022551.2, NP_072045.1
• RefSeq Size: 549 bp
• RefSeq ORF: 459 bp
• Locus ID: 6222
• UniProt ID: P62269
• Cytogenetics: 6p21.32
• Domains: Ribosomal_S13
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]