HLA (NM_022555) Human Untagged Clone

CAT#: SC112463

HLA (untagged)-Human major histocompatibility complex, class II, DR beta 3 (HLA-DRB3)

USD 196


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Size
    • 10 ug

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Specifications

Product Data
Product Name HLA (NM_022555) Human Untagged Clone
Symbol HLA
Synonyms HLA-DR3B
Vector pCMV6-XL5
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC112463 sequence for NM_022555 edited (data generated by NextGen Sequencing)
ATGGTGTGTCTGAAGCTCCCTGGAGGCTCCAGCTTGGCAGCGTTGACAGTGACACTGATG
GTGCTGAGCTCCCGACTGGCTTTCGCTGGGGACACCCGACCACGTTTCTTGGAGCTGCTT
AAGTCTGAGTGTCATTTCTTCAATGGGACGGAGCGGGTGCGGTTCCTGGAGAGACACTTC
CATAACCAGGAGGAGTACNTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGAGG
GAGCTGGGGCGGCCTGNNGCCGAGTACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAAG
CGGGGCCAGGTGGACAATTACTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTG
CAGCGGCGAGTCCATCCTCAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCAC
CACAACCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGG
TTCCGGAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACGGGCCTGATCCAGAATGGA
GACTGGACCTTCCAGACCCTGGTGATGCTAGAAACAGTTCCTCGGAGTGGAGAGGTTTAC
ACCTGCCAAGTGGAGCACCCAAGCGTAACGAGCCCTCTCACAGTGGAATGGAGTGCACGG
TCTGAATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTC
TTCCTTGGGGCCGGGCTGTTCATCTACTTCAGGAATCAGAAAGGACACTCTGGACTTCAG
CCAACAGGATTCCTGAGCTGA

Clone variation with respect to NM_022555.3
119 g=>t;164 a=>t;171 c=>g;175 t=>c;197 t=>a;199 c=>n;239 c=>g;257 t=>n;258 c=>n;266 c=>a;308 g=>a;603 t=>c;634 g=>c;654 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_022555 unedited
NNNNGGTTCGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGAGAC
TTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCTCCAGCATGGTGTGTCTGAAGCTC
CCTGGAGGCTCCAGCTTGGCAGCGTTGACAGTGACACTGATGGTGCTGAGCTCCCGACTG
GCTTTCGCTGGGGACACCCGACCACGTTTCTTGGAGCTGCTTAAGTCTGAGTGTCATTTC
TTCAATGGGACGGAGCGGGTGCGGTTCCTGGAGAGACACTTCCATAACCAGGAGGAGTAC
GCGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGAGGGAGCTGGGGCGGCCTGAT
GCCGAGTACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAAGCGGGGCCAGGTGGACAAT
TACTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTGCAGCGGCGAGTCCATCCT
CAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCACCACAACCTCCTGGTCTGC
TCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGGAACGGCCAGGAA
GAGAAGGCTGGNGGTGGTGTCCACGGGCCTGATCCAGAATGGAGACTGGACCTTCCAGAC
CCTGGTGATGCTAGAAACAGTTCCTCGGAGGGGNAGAAGGTTTTACACCTGCCAAGTGGA
GCACCCAAGCGTAACGAGCCCTCTCAAGTGGAATGGAGTGCACGGGTCTGATCTGCACAG
AGCAGATGCTGAGTGGAGTTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCTGGNGCCCGGG
CTGNTCATCTACTTCAGGAATCAGAAGACACTCTGGACTTAGCCACAGGATTCTGANCTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_022555 unedited
GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATATTTAA
CTCCATCTTTGAGAAAATTTAATAATGTAATGTGTTTGTGGTACAGGGTGAGTACAGATG
CACAGGAGGCCATAGGGTTTAGGCAAAGGGGAGCACAAAAGTTGAAGATGAGGCGCTGCC
ATCAATGCTGGGACTTCAGGCCAAGGGCAGGAACTGAGGAAGCCACAAGGGAGGACATTT
TCTGCAGTTGCTGAACCAGTAGCAACTAGGTCCTGAGAAAGCCCTCTCTCGTGGAAGAAT
AACAGCCAGGCGGGAAAGCTTTTCATCCTGCAAAGCTGGGGAAGAAGATTCTTCCTTAAA
TTGTCATCTGCACTTCAGCTCAAGAATCCTGTTGGCTGAAGTCCAGAGTGTCCTTTCTGA
TTCCTGAAGTAGATGAACAGCCCGGCCCCAAGGAAGAGCAGGCCCAGCACAAAGCCCCCG
ACTCCACTCAGCATCTTGCTCTGTGCAGATTCAGACCGTGCACTCCATTCCACTGTGAGA
GGGCTCGTTACGCTTGGGTGCTCCACTTGGCAGGTGTAAACCTCTCCACTCCGAGGAACT
GTTTCTAGCATCACCAGGGTCTGGAAGGTCCAGTCTCCATTCTGGATCAGGCCCGTGGAC
ACCACCCCAGCCTTCTCTTCCTGGCCGTTCCGGAACCACCTGACTTCAATGCTGCCTGGA
TAGAAACCACTCACAGAGCAAACCANGAAGTTGTGGTGCTGCAGGGGCTGGGTCTTTGCA
AGATACACAGTCACCCTGAGATGGACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCG
TAGNTGTGTCTGCAGTAATTTGTCCACTGGNCCCGCTTCTGCTCCAGNAGGTCCTTCGGG
CTGTTCAGTACTCGGNATTAAGCCGCCCCANTTCCTCACCGGCNGGTACTTCCCCACTGG
CTTTTGAAACCCCGTTCTTCTCCTGTTTTGGAT
Restriction Sites NotI-NotI     
Tag Tag Free
ACCN NM_022555
ORF Size 801 bp
Insert Size 1240
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reference Data
RefSeq NM_022555.3, NP_072049
RefSeq Size 1158
RefSeq ORF 801
Locus ID 3125
Cytogenetics 6p21.3
Domains MHC_II_beta, ig, IGc1
Protein Families Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Antigen processing and presentation, Hematopoietic cell lineage, Type I diabetes mellitus, Asthma, Autoimmune thyroid disease, Systemic lupus erythematosus, Allograft rejection, Graft-versus-host disease, Viral myocarditis
Gene Summary Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-ce [UniProtKB/Swiss-Prot Function]
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