E3 ubiquitin protein ligase MUL1 (MUL1) (NM_024544) Human Untagged Clone

CAT#: SC112170

MUL1 (untagged)-Human mitochondrial E3 ubiquitin protein ligase 1 (MUL1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E3 ubiquitin protein ligase MUL1
• Synonyms: C1orf166; GIDE; MAPL; MULAN; RNF218
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024544, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGAGCGGAGGGCGGCCCTCGCTGTGCCAGTTCATCCTCCTGGGCACCACCTCTGTGGTCACCGCCG
  CCCTGTACTCCGTGTACCGGCAGAAGGCCCGGGTCTCCCAAGAGCTCAAGGGAGCTAAAAAAGTTCATTT
  GGGTGAAGATTTAAAGAGTATTCTTTCAGAAGCTCCAGGAAAATGCGTGCCTTATGCTGTTATAGAAGGA
  GCTGTGCGGTCTGTTAAAGAAACGCTTAACAGCCAGTTTGTGGAAAACTGCAAGGGGGTAATTCAGCGGC
  TGACACTTCAGGAGCACAAGATGGTGTGGAATCGAACCACCCACCTTTGGAATGATTGCTCAAAGATCAT
  TCATCAGAGGACCAACACAGTGCCCTTTGACCTGGTGCCCCACGAGGATGGCGTGGATGTGGCTGTGCGA
  GTGCTGAAGCCCCTGGACTCAGTGGATCTGGGTCTAGAGACTGTGTATGAGAAGTTCCACCCCTCGATTC
  AGTCCTTCACCGATGTCATCGGCCACTACATCAGCGGTGAGCGGCCCAAAGGCATCCAAGAGACCGAGGA
  GATGCTGAAGGTGGGGGCCACCCTCACAGGGGTTGGCGAACTGGTCCTGGACAACAACTCTGTCCGCCTG
  CAGCCGCCCAAACAAGGCATGCAGTACTATCTAAGCAGCCAGGACTTCGACAGCCTGCTGCAGAGGCAGG
  AGTCGAGCGTCAGGCTCTGGAAGGTGCTGGCGCTGGTTTTTGGCTTTGCCACATGTGCCACCCTCTTCTT
  CATTCTCCGGAAGCAGTATCTGCAGCGGCAGGAGCGCCTGCGCCTCAAGCAGATGCAGGAGGAGTTCCAG
  GAGCATGAGGCCCAGCTGCTGAGCCGAGCCAAGCCTGAGGACAGGGAGAGTCTGAAGAGCGCCTGTGTAG
  TGTGTCTGAGCAGCTTCAAGTCCTGCGTCTTTCTGGAGTGTGGGCACGTTTGTTCCTGCACCGAGTGCTA
  CCGCGCCTTGCCAGAGCCCAAGAAGTGCCCTATCTGCAGACAGGCGATCACCCGGGTGATACCCCTGTAC
  AACAGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024544 unedited
  NGGGTTCGGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGGGGG
  GGTGCGGTCCTGGTCGGAAGGAGGTGGAGAGTCGGGGGTCACCAGGCCTATCCTTGGCGC
  CACAGTCGGCCACCGGGGCTCGCCGCCGTCATGGAGAGCGGAGGGCGGCCCTCGCTGTGC
  CAGTTCATCCTCCTGGGCACCACCTCTGTGGTCACCGCCGCCCTGTACTCCGTGTACCGG
  CAGAAGGCCCGGGTCTCCCAAGAGCTCAAGGGAGCTAAAAAAGTTCATTTGGGTGAAGAT
  TTAAAGAGTATTCTTTCAGAAGCTCCAGGAAAATGCGTGCCTTATGCTGTTATAGAAGGA
  GCTGTGCGGTCTGTTAAAGAAACGCTTAACAGCCAGTTTGTGGAAAACTGCAAGGGGGTA
  ATTCAGCGGCTGACACTTCAGGAGCACAAGATGGTGTGGAATCGAACCACCCACCTTTGG
  AATGATTGCTCAAAGATCATTCATCAGAGGACCAACACAGTGCCCTTTGACCTGGTGCCC
  CACGAGGATGGCGTGGATGTGGCTGTGCGAGTGCTGAAGCCCCTGGACTCAGTGGATCTG
  GGTCTAGAGACTGTGTATGAGAAGTTCCACCCCTCGATTCAGTCCTTCACCGATGTCATC
  GGCCACTACATCAGCGGTGAGCGGCCCNAGGCATCCAAGAGACCGAGGAGATGCTGAAGG
  TGGGGGCCACCCTCACAGGGGTTGGCGAACTGGTCCTGGNACACACTCTGTCCGCCTGCA
  GCCGCCCAAACAGGCATGCAGTACTATCTAAAGCAGCCAGACTTCGACAGCCTGCTGCAG
  AAGCAGGAGTCGAGCGTCAGCTCTGGAAGGTGCNNTGCGCTGTTTTTGGCTTGCACATGT
  GCACCCTCTTCTTCATTCTCGAAGCAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024544 unedited
  GTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTGGTTGCAACATGTT
  TAATTTCTGCTGTTCACACTGGACACTGCATCATACTAGTGTCGGCCCCTGAGGGCACCC
  CTTCCTCGCCTGCACAAAGGAGGACGAGAGATGAACATTCAGAGGCAGAAAAGGGCAATA
  AAAAAAGAGCTGTGTATGTGACCTCCAACTACTCAGAGGTGGGGGAAAACAGCCCCATCT
  GTCTTGCACTAAAAGGCTCACCAAGGGCAGGTGAGGGGCAAATGGTAATACTGGGAGGGG
  GTAACACAAGGAGAAGCGACATGAGTACACCAAGATGTCAAAGCTGCGACGGGCTGGATG
  AAGGAGCCCCAAGAGGGCATATGCTCAGGGTGCCAGCCGGCTGCTTTTCCTTGTGACAGC
  CTTGCAAGGAAGCTGTGAGGCCAGGACACTAAGCCAGTGGCATCCACACTGCGTCCTTCA
  AGAAGCGAGGCCTCTGCGCCTCATTCTCCAGGAGGGAAAACCAGGGACCACCCCGAGCTC
  TCCCAAGTGGCAGGAAGTTAAATCAGGGCCAGTGGCGAATCCAGGAAGCCTGCCTGACCA
  ACTGCCCTTAACATGAATCTTTCTTAATTCTGGTGCCCGGGTGCGCGCGATCTTCTGGGG
  AGAATGCGGATGTGCTGACTTACATTTCCTTTGTTCTCCTACCCCACTGGGCGGCGGCTC
  CGGTGGTCAGCCAGGCGAGGGACAGGAATAAAAATCGTCTCGTCCCACATTGTCATTTTC
  TAATTATCCTCTTTTCTCCATCCGCTTGTTAACAAAATAATAATCCCTGTGCTGCATCTT
  TTCTCGGGACCTCCCCACCCGCGTGTGTTTGGCCTCGCGCCCCAATCTTTACATCTCTCC
  GTCAGGTTCCCCACATCACCTCTCCCACCCCTCNCNCGCTAATACTTCTCTCCCCCGCTC
  CCTACGCGCCCTGCTTACTCCACTGCTCGCTGCCCTTTCCCCGCGTTGTCCCTATCCTCT
  AGCTATTCAGTTCATCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024544
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024544.1, NP_078820.1
• RefSeq Size: 2442 bp
• RefSeq ORF: 1059 bp
• Locus ID: 79594
• UniProt ID: Q969V5
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Exhibits weak E3 ubiquitin-protein ligase activity (PubMed:18591963, PubMed:19407830, PubMed:22410793). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed:18591963, PubMed:19407830, PubMed:22410793). Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation (PubMed:22410793). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (PubMed:19407830). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial localization (PubMed:19407830, PubMed:18207745, PubMed:18213395). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed:24898855). May also be involved in the sumoylation of the membrane fission protein DNM1L (PubMed:18207745, PubMed:19407830). Inhibits cell growth (PubMed:18591963, PubMed:22410793). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis (PubMed:23399697). Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response (PubMed:23399697). Can mediate DDX58 sumoylation and disrupt its polyubiquitination (PubMed:23399697).[UniProtKB/Swiss-Prot Function]