LACTB (NM_032857) Human Untagged Clone

CAT#: SC111862

LACTB (untagged)-Human lactamase, beta (LACTB), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LACTB
• Synonyms: G24; MRPL56
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032857, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACCGGCTCATGTCAGCAGTGACTGCCCGGGCTGCCGCCCCCGGGGGCTTGGCCTCAAGCTGCGGAC
  GACGCGGGGTCCATCAGCGCGCCGGGCTGCCGCCTCTCGGCCACGGCTGGGTCGGGGGCCTCGGGCTGGG
  GCTGGGGCTGGCGCTCGGGGTGAAGCTGGCAGGTGGGCTGAGGGGCGCGGCCCCGGCGCAGTCCCCCGCG
  GCCCCCGACCCTGAGGCGTCGCCTCTGGCCGAGCCGCCACAGGAGCAGTCCCTCGCCCCGTGGTCTCCGC
  AGACCCCGGCGCCGCCCTGCTCCAGGTGCTTCGCCAGAGCCATCGAGAGCAGCCGCGACCTGCTGCACAG
  GATCAAGGATGAGGTGGGCGCACCGGGCATAGTGGTTGGAGTTTCTGTAGATGGAAAAGAAGTCTGGTCA
  GAAGGTTTAGGTTATGCTGATGTTGAGAACCGTGTACCATGTAAACCAGAGACAGTTATGCGAATTGCTA
  GCATCAGCAAAAGTCTCACCATGGTTGCTCTTGCCAAATTGTGGGAAGCAGGGAAACTGGATCTTGATAT
  TCCAGTACAACATTATGTTCCCGAATTCCCAGAAAAAGAATATGAAGGTGAAAAGGTTTCTGTCACAACA
  AGATTACTGATTTCCCATTTAAGTGGAATTCGTCATTATGAAAAGGACATAAAAAAGGTGAAAGAAGAGA
  AAGCTTATAAAGCCTTGAAGATGATGAAAGAGAATGTTGCATTTGAGCAAGAAAAAGAAGGCAAAAGTAA
  TGAAAAGAATGATTTTACTAAATTTAAAACAGAGCAGGAGAATGAAGCCAAATGCCGGAATTCAAAACCT
  GGCAAGAAAAAGAATGATTTTGAACAAGGCGAATTATATTTGAGAGAAAAGTTTGAAAATTCAATTGAAT
  CCCTAAGATTATTTAAAAATGATCCTTTGTTCTTCAAACCTGGTAGTCAGTTTTTGTATTCAACTTTTGG
  CTATACCCTACTGGCAGCCATAGTAGAGAGAGCTTCAGGATGTAAATATTTGGACTATATGCAGAAAATA
  TTCCATGACTTGGATATGCTGACGACTGTGCAGGAAGAAAACGAGCCAGTGATTTACAATAGAGCAAGAT
  TTTATGTTTACAATAAAAAGAAACGTCTTGTCAACACACCTTACGTGGATAACTCCTATAAATGGGCTGG
  TGGTGGATTTCTGTCTACAGTGGGTGACCTTCTGAAATTTGGGAATGCAATGCTTTATGGTTACCAAGTT
  GGGCTGTTTAAGAACTCAAATGAAAATCTTTTACCTGGATACCTCAAACCAGAAACAATGGTTATGATGT
  GGACCCCAGTCCCTAACACAGAGATGTCTTGGGATAAAGAGGGTAAATATGCAATGGCGTGGGGTGTTGT
  GGAAAGGAAACAAACGTATGGTTCGTGTAGAAAGCAACGGCATTATGCTTCACATACTGGAGGGGCAGTG
  GGTGCCAGTAGTGTCCTGCTGGTCCTTCCTGAAGAACTGGATACAGAGACTATAAATAACAAGGTTCCCC
  CAAGAGGAATCATTGTTTCTATCATATGTAACATGCAATCTGTTGGCCTCAATAGCACCGCTTTGAAGAT
  TGCCCTTGAATTTGATAAAGACAGATCAGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032857 unedited
  GATATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGACGCCATGT
  ACCGGCTCCTGTCAGCAGTGACTGCCCGGGCTGCCGCCCCCGGGGGCTTGGCCTCAAGCT
  GCGGACGACGCGGGGTCCATCAGCGCGCCGGGCTGCCGCCTCTCGGCACGGTGGGTTCGN
  GGGCCTCGGGCTGGGGCTGGGGCTGGCGCTCGGGGTGAAGCTGGCAGGTGGGCTGAGGGG
  CGCGGCCCCGGCGCAGTCCCCCGCGGCCCCCGACCCTGAGGCGTCGCCTCTGGCCGAGCC
  GCCACAGGAGCAGTCCCTCGCCCCGTGGTCTCCGCAGACCCCGGCGCCGCCCTGCTCCAG
  GTGCTTCGCCAGAGCCATCGAGAGCAGCCGCGACCTGCTGCACAGGATCAAGGATGAGGT
  GGGCGCACCGGGCATAGTGGTTGGAGTTTCTGTAGATGGAAAAGAAGTCTGGTCAGAAGG
  TTTAGGTTATGCTGATGTTGAGAACCGTGTACCATGTAAACCAAAGACAGTTATGCGAAT
  TGCTAGCATCAGCAAAAGTCTCACCATGGTTGCTCTTGCCAAATTGTGGGAAGCAGGGAA
  ACTGGATCTTGATATTTCAGTAAAACATTATGTTCCCGAATTCCCAAAAAAAGAATATGA
  AGGTGAAAAGGTTTCTGTTAAAACAAGATTACTGATTTTTCATTTAAAGGGGAATCTTTC
  ATTATGAAAAAGACATAATAAAGGTGAAAGAAGAAAAAACTTATTAAAGCTTTTGAAAAG
  ATCAAAAATAAATGTTGCATTTTGTTCTATAAAAATAACGCCAAAATTATGGAAAAAAAG
  GATTTTATTAAATTAAAACAGAGCACGGATAATGAAGCCAAAAGCCGGAATTAAAACTTT
  GCAGAAAAAAAAAGATTTTTAACAAGGCAATTAATTTGAAAGAAAAATCGAAACATCATG
  ATCCCCAAAAATATAAAAAAATCTTTTTTTTTCAACTCGATAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032857
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032857.2, NP_116246.2
• RefSeq Size: 1956 bp
• RefSeq ORF: 1644 bp
• Locus ID: 114294
• UniProt ID: P83111
• Cytogenetics: 15q22.2
• Domains: beta-lactamase
• Protein Families: Protease
• Gene Summary: This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (a).