MAP1LC3A (NM_032514) Human Untagged Clone

CAT#: SC111851

MAP1LC3A (untagged)-Human microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP1LC3A
• Synonyms: ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111851 sequence for NM_032514 edited (data generated by NextGen Sequencing)
  ATGCCCTCAGACCGGCCTTTCAAGCAGCGGCGGAGCTTCGCCGACCGCTGTAAGGAGGTA
  CAGCAGATCCGCGACCAGCACCCCAGCAAAATCCCGGTGATCATCGAGCGCTACAAGGGT
  GAGAAGCAGCTGCCCGTCCTGGACAAGACCAAGTTTTTGGTCCCGGACCATGTCAACATG
  AGCGAGTTGGTCAAGATCATCCGGCGCCGCCTGCAGCTGAACCCCACGCAGGCCTTCTTC
  CTGCTGGTGAACCAGCACAGCATGGTGAGTGTGTCCACGCCCATCGCGGACATCTACGAG
  CAGGAGAAAGACGAGGACGGCTTCCTCTATATGGTCTACGCCTCCCAGGAAACCTTCGGC
  TTCTGA
  
  Clone variation with respect to NM_032514.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032514 unedited
  TCACATATTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGAGC
  CCCCAAACCGCAGACACATCCCCGCGCCCCAGAGCCCCGGCCTGCGCGCCCAGCCGGGCC
  CGCGCGATGCCCTCAGACCGGCCTTTCAAGCAGCGGCGGAGCTTCGCCGACCGCTGTAAG
  GAGGTACAGCAGATCCGCGACCAGCACCCCAGCAAAATCCCGGTGATCATCGAGCGCTAC
  AAGGGTGAGAAGCAGCTGCCCGTCCTGGACAAGACCAAGTTTTTGGTCCCGGACCATGTC
  AACATGAGCGAGTTGGTCAAGATCATCCGGCGCCGCCTGCAGCTGAACCCCACGCAGGCC
  TTCTTCCTGCTGGTGAACCAGCACAGCATGGTGAGTGTGTCCACGCCCATCGCGGACATC
  TACGAGCAGGAGAAAGACGAGGACGGCTTCCTCTATATGGTCTACGCCTCCCAGGAAACC
  TTCGGCTTCTGAGCCAGCAGTAGGGGGGCTCGGCCTGGGAGTCGGGGGGCCCCGGTCAGG
  CCCTGCCCAGAGAGCTCCTGGTTCCTGAACTGAGCTGCCTCTACCGTGGTGGGCTGGGCA
  GGCATGTGCCCCCCTAGTCAGAGGGCACCAACCCACCTACTCTGCCCCTGNGTGGATCCT
  GNGCCGGTCGTGTTAGGGTTGTCCCTCTGGGTGCTGGCTGGTGGGATGGGGANNGGTGGG
  GGAGCAACTTCCAGCACCCCTGCTGTGTGGTTCATCTTTTTTTTAGGCCCCTGCCTGTCT
  GCCCATCTGGCCCCTCACCCACCCCGAGCTCTGCCCACCGCNTGGNACCTGCCCCACCCC
  TGAAAAGACTGGCCCCCTGGCTCCCGNCCCCTCGGGTCTCACGTGGNNGTAATGGATCTG
  TGGGTCATTGGNCCCTCTTGCANAATAAAGATTGCTCAGCCCTGCCCTGGCCCTGTGAAA
  NNAAANNAANNNNNNNNNNANTAATTAACACACAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032514
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032514.2, NP_115903.1
• RefSeq Size: 1030 bp
• RefSeq ORF: 366 bp
• Locus ID: 84557
• UniProt ID: Q9H492
• Cytogenetics: 20q11.22
• Domains: MAP1_LC3
• Gene Summary: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) encodes the predominant isoform (a).