MCM10 (NM_018518) Human Untagged Clone

CAT#: SC111824

MCM10 (untagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM10
• Synonyms: CNA43; DNA43; PRO2249
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018518, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATGAGGAGGAAGACAATCTGTCTCTGCTGACCGCACTGCTGGAAGAAAATGAGTCAGCCTTGGATT
  GTAATTCAGAAGAAAATAACTTCTTGACGCGGGAAAATGGCGAGCCCGACGCATTTGATGAGCTCTTTGA
  TGCCGACGGCGACGGTGAATCTTATACAGAAGAGGCTGATGATGGAGAAACAGGAGAGACAAGAGACGAA
  AAGGAAAATCTGGCCACTCTCTTTGGAGATATGGAGGACTTAACAGATGAAGAAGAAGTTCCCGCATCAC
  AGTCAACTGAAAATAGGGTCCTCCCTGCTCCTGCCCCCAGGCGAGAGAAAACGAATGAAGAGTTGCAAGA
  GGAATTAAGGAATTTGCAAGAGCAAATGAAGGCCTTACAAGAGCAGCTAAAAGTAACAACAATTAAACAG
  ACAGCAAGCCCAGCCCGTCTGCAAAAATCCCCTGAGAAGTCTCCCCGGCCACCTCTTAAGGAGAGGAGAG
  TTCAGAGAATTCAGGAGTCAACATGCTTTTCTGCGGAGCTTGATGTCCCTGCGCTACCAAGAACCAAGAG
  GGTGGCTCGAACACCAAAGGCTTCACCTCCAGATCCCAAAAGCTCATCTTCAAGGATGACAAGTGCACCC
  TCCCAACCCCTACAGACGATTTCTCGGAACAAACCTAGTGGGATAACTAGAGGTCAAATTGTGGGGACCC
  CAGGAAGTTCTGGGGAAACGACTCAACCCATCTGTGTGGAAGCCTTCTCTGGTCTGCGGCTCAGGCGGCC
  TCGAGTATCCTCCACAGAAATGAACAAGAAAATGACCGGCCGAAAACTGATCAGACTGTCTCAGATCAAG
  GAAAAGATGGCCAGAGAGAAGCTGGAAGAAATAGATTGGGTGACATTTGGGGTTATATTGAAGAAGGTTA
  CGCCACAGAGTGTGAATAGTGGAAAAACCTTCAGCATATGGAAACTGAATGATCTTCGTGACCTGACACA
  ATGTGTGTCCTTGTTCTTATTTGGAGAAGTTCACAAAGCGCTCTGGAAGACGGAGCAGGGGACTGTCGTA
  GGGATCCTCAATGCCAACCCCATGAAGCCCAAGGATGGTTCAGAGGAGGTGTGTTTATCTATCGATCATC
  CTCAGAAGGTCTTAATTATGGGTGAAGCTCTTGACCTGGGAACCTGTAAAGCCAAGAAGAAGAATGGAGA
  GCCGTGCACGCAGACTGTGAATTTGCGTGACTGTGAGTACTGTCAGTACCATGTCCAGGCTCAGTACAAG
  AAGCTCAGCGCAAAGCGTGCGGATCTGCAGTCCACCTTCTCTGGAGGACGAATTCCAAAGAAGTTTGCCC
  GCAGAGGCACCAGCCTCAAAGAACGGCTGTGCCAAGATGGCTTTTACTACGGAGGGGTTTCTTCTGCCTC
  GTATGCAGCTTCAATTGCAGCAGCTGTGGCTCCTAAGAAGAAGATTCAAACCACTCTGAGTAATCTGGTT
  GTTAAGGGCACAAACTTGATCATCCAGGAAACACGGCAAAAACTCGGAATACCCCAGAAGAGCCTGTCTT
  GCTCTGAGGAGTTCAAGGAACTGATGGACCTGCCGACGTGTGGAGCCAGGAACTTAAAACAACATTTAGC
  CAAAGCCACAGCTTCAGGGATTATGGGGAGCCCAAAACCAGCCATCAAGTCCATCTCGGCCTCAGCACTC
  TTGAAGCAACAGAAGCAGCGGATGTTGGAGATGAGGAGAAGGAAATCAGAAGAAATACAGAAGCGATTTC
  TGCAGAGCTCAAGTGAAGTTGAGAGCCCAGCTGTGCCATCTTCATCAAGACAGCCCCCTGCTCAGCCTCC
  ACGGACAGGATCCGAGTTCCCCAGGCTGGAGGGAGCCCCGGCCACAATGACGCCCAAGCTGGGGCGAGGT
  GTCTTGGAAGGAGATGATGTTCTCTTTTATGATGAGTCACCACCACCAAGACCAAAACTGAGTGCTTTAG
  CAGAAGCCAAAAAGTTAGCTGCTATCACCAAATTAAGGGCAAAAGGCCAGGTTCTTACAAAAACAAACCC
  AAACAGCATTAAGAAGAAACAAAAGGACCCTCAGGACATCCTGGAGGTGAAGGAACGTGTAGAAAAAAAC
  ACCATGTTTTCTTCTCAAGCTGAGGATGAATTGGAGCCTGCCAGGAAAAAAAGGAGAGAACAACTTGCCT
  ATCTGGAATCTGAGGAATTTCAGAAAATCCTAAAAGCAAAATCAAAACACACAGGCATCCTGAAAGAGGC
  CGAGGCTGAGATGCAGGAGCGCTACTTTGAGCCACTGGTGAAAAAAGAACAAATGGAAGAAAAGATGAGA
  AACATCAGAGAAGTGAAGTGCCGTGTCGTGACATGCAAGACGTGCGCCTATACCCACTTCAAGCTGCTGG
  AGACCTGCGTCAGTGAGCAGCATGAATACCACTGGCATGATGGTGTGAAGAGGTTTTTCAAATGTCCCTG
  TGGAAACAGAAGCATCTCCTTGGACAGACTCCCGAACAAGCACTGCAGTAACTGTGGCCTCTACAAATGG
  GAACGGGACGGAATGCTAAAGGAAAAGACTGGTCCAAAGATAGGAGGAGAAACTCTGTTACCAAGAGGAG
  AAGAACATGCTAAATTTCTGAACAGCCTTAAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018518 unedited
  NTTTTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCGGCATCGGCCAAGATTCTACATTGCTCATCTGGGCATCTGAG
  CCTCCTTCGAAGTTTCCTGTCACAACTGTCCTCTTGACAGCATGGATGAGGAGGAAGACA
  ATCTGTCTCTGCTGACCGCACTGCTGGAAGAAAATGAGTCAGCCTTGGATTGTAATTCAG
  AAGAAAATAACTTCTTGACGCGGGAAAATGGCGAGCCCGACGCATTTGATGAGCTCTTTG
  ATGCCGACGGCGACGGTGAATCTTATACAGAAGAGGCTGATGATGGAGAAACAGGAGAGA
  CAAGAGACGAAAAGGAAAATCTGGCCACTCTCTTTGGAGATATGGAGGACTTAACAGATG
  AAGAAGAAGTTCCCGCATCACAGTCAACTGAAAATAGGGTCCTCCCTGCTCCTGCCCCCA
  GGCGAGAGAAAACGAATGAAGAGTTGCAAGAGGAATTAAGGAATTTGCAAGAGCAAATGA
  AGGCCTTACAAGAGCAGCTAAAAGTAACAACAATTAAACAGACAGCAAGCCCAGCCCGTC
  TGCAAAAATCCCCTGAGAAGTCTCCCCGGCCACCTCTTAAGGAGAGGAGAGTTCAGAGAT
  TTCAGGAGTCAACATGCTTTTCTGCGGAGCTTGATGTCCCTGCGCTACCAAAACCAAGAA
  GGTGGCTCGAACCCCAAAGGCTTCACCTCCCTATCCCAAAGCTCATCTTTCAGGGAGACA
  AGTGCACCCTCCCACCCCTACAGACGATTTTTCGGACAAACCTATGGGATAACTACAAGG
  CAAATGTTGGGGACCCCAGCAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018518 unedited
  NNGGGTACCACTATGNACCCGGNCCGCAANCTANGATCGNATTTTTTTTTTTTTTTTTTG
  GAAGCAAAGTGGGTGGATTGCTAGTGCTCAGGAGTTTGAGACCAGCCTGAGCAACATGGC
  AAAACCCCATCTCTACAAAAAATGCAAAAATTAGCTGGGCTTGGTCACGCACGCCTGTAG
  TCCTAGCTATTTGGGAGACTGAGGTGGGAGGACTGCTTGAGCCCAGGGAAGCCAAGGCTG
  CAGTGTGCCGCGATCGCACTACTGCACTCCAGCCAGGGTGACATAGTGAGACCCTGTCAT
  AAAAAATAAAAAAATTAAAAAAAAAATAATATGAGATTTTACATATTTCTATCACAGGGC
  TCCACAATAATGTAAGAGCAGACAGCCTTCTTTCTCTCTCTATATATAAACTGAAATGCA
  AAGTGATTCAGTGGCTTGATCAATTTTCAAGAGTAAAACAGAGATAACAGAATTGAATGA
  AGAAATCTGGCTTTTAATGGCCCTACATAAAGACTATGTGATCATCATTATCTAGATGGG
  AAAGTTTTATTATTTGTAGAAATCCCCTGCTTAAAAAAACATTTTAAGGAATCCTTAATC
  CCAACTTCCCGGATTTTCACGATGAAAAAACCGAAGCCCCAAAATTAAACTATTTACCTG
  GGAGGGGGAAAAAACCCCAATTTTTTTGCCCCTTGGGGGGTGTCATAAAACACTAAAGAT
  TTTGTTATTGTTAAAAAAGTGAACACCTTTTTTAACCAAAAGATAACGAAACCTCCCCCC
  CTTTGGTAACCCCCCCCATTTTTGGCCCCTAAACTCTTTGGATGAAATAAGCGCACTCTA
  CACCCCGTGGTTTTTTATAAAACATATTTACCTTGGGTTCCCCCTTAAGGAGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018518
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018518.3, NP_060988.3
• RefSeq Size: 4532 bp
• RefSeq ORF: 2625 bp
• Locus ID: 55388
• UniProt ID: Q7L590
• Cytogenetics: 10p13
• Protein Families: Stem cell - Pluripotency
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) encodes the predominant isoform (2).