UBE2H (NM_003344) Human Untagged Clone

CAT#: SC111701

UBE2H (untagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2H
• Synonyms: E2-20K; GID3; UBC8; UBCH; UBCH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003344 edited
  ATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAG
  AGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAAGTTTTATGGA
  CCACAAGGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAA
  TACCCTTTCAAATCTCCATCTATAGGATTCATGAATAAAATTTTCCATCCCAACATTGAT
  GAAGCGTCAGGAACTGTGTGTCTAGATGTAATTAATCAAACTTGGACAGCTCTCTATGAT
  CTTACCAATATATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTATCCTAACCCCATAGAT
  CCTCTCAATGGTGACGCTGCAGCCATGTACCTCCACCGACCAGAAGAATACAAGCAGAAA
  ATTAAAGAGTACATCCAGAAATACGCCACGGAGGAGGCGCTGAAAGAACAGGAAGAGGGT
  ACCGGGGACAGCTCATCGGAGAGCTCTATGTCTGACTTTTCCGAAGATGAGGCCCAGGAT
  ATGGAGTTGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003344 unedited
  ATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAA
  TTCGGCACGAGGACCATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTG
  GTCAAGCTCATCGAGAGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTA
  GTGAAGTTTTATGGACCACAAGGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTG
  GACCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTCATGAATAAAATTTTC
  CATCCCAACATTGATGAAGCGTCAGGAACTGTGTGTCTAGATGTAATTAATCAAACTTGG
  ACAGCTCTCTATGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTAT
  CCTAACCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTCCACCGACCAGAA
  GAATACAAGCAGAAAATTAAAGAGTACATCCAGAAATACGCCACGGAGGAGGCGCTGAAA
  GAACAGGAAGAGGGTACCGGGGACAGCTCATCGGAGAGCTCTATGTCTGACTTTTCCGAA
  GATGAGGCCCAGGATATGGAGTTGTAGTAGAAAAAGCACCTGCTTTTCAGAAAGACTATT
  ATTTCCTAACCATGAGAAGCAGACTATAATATTCATATTTAAACCAAAGCATTTTTTTTA
  TTACTAAACAAGGTTTTTATGAATAATAGCATTGATATATATATATTATATATCACCCTT
  TAGATCTTGATTTCTTGGTCATTTCTCAACCTGAGGTGCATAGCATATTCCCACATTCCA
  TTTGGTAGCAATATGCGGTCTGAATGCATGCATTCATGAGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003344 unedited
  ATTTGGCCGCGGCCGCATTTTANATCGAGTTTTTTTTTTTTTTTTTTAAAGAAACCTTTT
  GCTTTTGCCCGTAATTAACATAAAGGAGGGAAATTCTTTGTCTTTTTATTTAGGAAAATA
  ATCATGCTAAAAGCAAGTTGTACTTTATATAGATTTTCAAACAAAAGATTGATTGTGCTT
  TTTGAATAAAAAAGATAGGGTATCTTCCAACCTCATTTTTTTAAACCTTGTGGAATACAG
  CACTTGTAAACTAAAAACACTCTTTTTAAAGAAAAATTAAATAATCCCAGTGGGATGCTC
  AAAAATCGTCTTTAGAAGGTTTTAGGATTGGCTAATTCTGCTATTATCTTATTGGCTGCG
  GCAAAAAAAGAACACAGAGCTGCTGTCTAAAATTTCTCCAAACTCCCCTCCGAGTTCAGC
  AGGCAACACTCAGATTTGAAAGAAACTCTGCAGCAACCTGGACATATGGTTAGCTGAGTC
  GACATTTGATTAGCAGGCATTTTAAACTGATTTCCTCTTAACATACGCAGGAGAGAAAAC
  ATTCTCTTCCTGCAGGGCATGTTCTACTACATCATATTTCTGAATCCCTTCTTCCTATTC
  GGTCACTCTCTTGAAAAAGCTGGTTTGNTTTTCCCATGTAGAATCACTCTGGGTACAAGA
  ACCTTTATTTGTTCCAATTATTATTTGCTTTTAATTTAGAACCTGGTGTGGGGTCTAAAA
  TACCATTTCCCACTCCCACCACCATATGATGACCAAGCCTGCTGCCAAGAGCCACCAAAA
  AAGGGGAAACCCAACCCCGACATGAAGGCCAGGTCCCAAGGGGTTTGGAGTCACCCAGGG
  GGTCGGTCTTCTCAGGCTTAAGGCCCATGAACCGCTTGTTCAAGGAGGCGCCGCCTCTTG
  GCAGGGAAAAAAAATTTGGAACCTTTTTTGCCCATTCCCCCACCCCCCCCCAAAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003344
• Insert Size: 310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003344.2, NP_003335.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 552 bp
• Locus ID: 7328
• UniProt ID: P62256
• Cytogenetics: 7q32.2
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.