PSMD4 (NM_002810) Human Untagged Clone

CAT#: SC111678

PSMD4 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD4
• Synonyms: AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111678 sequence for NM_002810 edited (data generated by NextGen Sequencing)
  ATGGTGTTGGAAAGCACTATGGTGTGTGTGGACAACAGTGAGTATATGCGGAATGGAGAC
  TTCTTACCCACCAGGCTGCAGGCCCAGCAGGATGCTGTCAACATAGTTTGTCATTCAAAG
  ACCCGCAGCAACCCTGAGAACAACGTGGGCCTTATCACACTGGCTAATGACTGTGAAGTG
  CTGACCACACTCACCCCAGACACTGGCCGTATCCTGTCCAAGCTACATACTGTCCAACCC
  AAGGGCAAGATCACCTTCTGCACGGGCATCCGCGTGGCCCATCTGGCTCTGAAGCACCGA
  CAAGGCAAGAATCACAAGATGCGCATCATTGCCTTTGTGGGAAGCCCAGTGGAGGACAAT
  GAGAAGGATCTGGTGAAACTGGCTAAACGCCTCAAGAAGGAGAAAGTAAATGTTGACATT
  ATCAATTTTGGGGAAGAGGAGGTGAACACAGAAAAGCTGACAGCCTTTGTAAACACGTTG
  AATGGCAAAGATGGAACCGGTTCTCATCTGGTGACAGTGCCTCCTGGGCCCAGTTTGGCT
  GATGCTCTCATCAGTTCTCCGATTTTGGCTGGTGAAGGTGGTGCCATGCTGGGTCTTGGT
  GCCAGTGACTTTGAATTTGGAGTAGATCCCAGTGCTGATCCTGAGCTGGCCTTGGCCCTT
  CGTGTATCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAGGAGGCCCGGCGGGCAGCTGCA
  GCTTCTGCTGCTGAGGCCGGGATTGCTACGACTGGGACTGAAGACTCAGACGATGCCCTG
  CTGAAGATGACCATCAGCCAGCAAGAGTTTGGCCGCACTGGGCTTCCTGACCTAAGCAGT
  ATGACTGAGGAAGAGCAGATTGCTTATGCCATGCAGATGTCCCTGCAGGGAGCAGAGTTT
  GGCCAGGCGGAATCAGCAGACATTGATGCCAGCTCAGCTATGGACACATCCGAGCCAGCC
  AAGGAGGAGGATGATTACGACGTGATGCAGGACCCCGAGTTCCTTCAGAGTGTCCTAGAG
  AACCTCCCAGGTGTGGATCCCAACAATGAAGCCATTCGAAATGCTATGGGCTCCCTGGCC
  TCCCAGGCCACCAAGGACGGCAAGAAGGACAAGAAGGAGGAAGACAAGAAGTGA
  
  Clone variation with respect to NM_002810.2
  951 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002810 unedited
  NAACGGTTCCAANNCATATTTGGTNNAATNACCGNAACTNCACTTATNNAGGGNNCGGCN
  NCGCGNAATTNCGGNCACGAAGGGNAGGNNAAGGTGGCAAGNAATGGTGTTGGNAAAGCA
  CTATGGTGTGTGTGGACAACAGTGAGTATATGCGGAATGGAGACTTCTTACCCACCAGGC
  TGCAGGCCCAGCAGGATGCTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTG
  AGAACAACGTGGGCCTTATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCACCC
  CAGACACTGGCCGTATCCTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACCT
  TCTGCACGGGCATCCGCGTGGCCCATCTGGCTCTGAAGCACCGACAAGGCAAGAATCACA
  AGATGCGCATCATTGCCTTTGTGGGAAGCCCAGTGGAGGACAATGAGAAGGATCTGGTGA
  AACTGGCTAAACGCCTCAAGAAGGAGAAAGTAAATGTTGACATTATCAATTTTGGGGAAG
  AGGAGGTGAACACAGAAAAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAGATGGAA
  CCGGTTCTCATCTGGTGACAGTGCCTCCTGGGCCCAGTTTGGCTGATGCTCTCATCAGTT
  CTCCGATTTTGGCTGGTGAAGGTGGTGCCATGCTGGGTCTTGGTGCCAGTGACTNTGAAT
  TTGGAGTAGATCCCAGTGCTGATCCTGAGCTGGCCTTGGCCCTTCGTGTATCTATGGAAA
  AGCANCGCANNNCGCANNGAGAGGGAGGCCCCGCGGCCAGCTGCAGCTTCTGCTGCTNGA
  GCCGGGATTGCTACGACTGGGACTGAAGACTCANACGATGCCCTGCTTGAGATGACCATC
  AGCANCCAAGAATTGGNCCGCACTGGGCTTTCTGACCTAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002810 unedited
  GGACCTTGGAATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGC
  CACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTT
  TTTTTTTTTTTTTTTAAAAGTTGCCAAGCTTTATTTAGGCTGTAATGGTTACAGATAACA
  CACATCTAACCCTATATATCCGTGCTTCCCATGCAGTCCCCTAAGCAGACTCAGCTACCC
  TTTCCCTCCAGTCTCACTTCTTGTCTTCCTCCTTCTTGTCCTTCTTGCCGTCCTTGGTGG
  CCTGGGAGGCCAGGGAGCCCATAGCATTTCGAATGGCTTCATTGTTGGGATCCACACCTG
  GGAGGTTCTCTAGGACACTCTGAAGGAACTCGGGGTCCTGCATCACGTCGTAATCATCCT
  CCTCCTTGGCTGGCTCGGATGTGTCCATAGCTGAGCTGGCATCAATGTCTGCTGATTCCG
  CCTGGCCAAACTCTGCTCCCTGCAGGACATCTGCATGGCATAAGCAATCTGCTCTTCCTC
  AGTCATACTGCTTAGGTCAGGAAGCCCAGTGCGGCCAAACATTTGCTGGCTGATGGTCAT
  CTTCAGCAAGGGCATCGTCTGAGTCCTCCAATCCCAGTCCGTAGCAAATCCCGGCCTTAG
  CAGCAAAAGCTGCACCTGCCCGCCGGGCCTCCTCCTCCCTGCGATTCCGGCTGCTTTTTC
  ATAGAAACCCCGAAGGGCCCAGGCCCACTTAAGGTCAGCACTGGGATCCTTCTCCAATTG
  AAGGTCCGGGGACACAAGACCCGTATGGGAACCTCTTAACCGCGCAAATGCGAAAACTTG
  TTCAAGAATCGCCAAATTTGGCCCCACGGACACTGCACCAAAAGATAAACCGTTTCCTTT
  CTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002810
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002810.1, NP_002801.1
• RefSeq Size: 1313 bp
• RefSeq ORF: 1134 bp
• Locus ID: 5710
• UniProt ID: P55036
• Cytogenetics: 1q21.3
• Domains: VWA, UIM
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.