ATP5PD (NM_006356) Human Untagged Clone

CAT#: SC111154

ATP5H (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d (ATP5H), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5PD
• Synonyms: APT5H; ATP5H; ATPQ
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111154 sequence for NM_006356 edited (data generated by NextGen Sequencing)
  ATGGCTGGGCGAAAACTTGCTCTAAAAACCATTGACTGGGTAGCTTTTGCAGAGATCATA
  CCCCAGAACCAAAAGGCCATTGCTAGTTCCCTGAAATCCTGGAATGAGACCCTCACCTCC
  AGGTTGGCTGCTTTACCTGAGAATCCACCAGCTATCGACTGGGCTTACTACAAGGCCAAT
  GTGGCCAAGGCTGGCTTGGTGGATGACTTTGAGAAGAAGTTTAATGCGCTGAAGGTTCCC
  GTGCCAGAGGATAAATATACTGCCCAGGTGGATGCCGAAGAAAAAGAAGATGTGAAATCT
  TGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGGATTGTAGAATATGAGAAAGAGATGGAG
  AAGATGAAGAACTTAATTCCATTTGATCAGATGACCATTGAGGACTTGAATGAAGCTTTC
  CCAGAAACCAAATTAGACAAGAAAAAGTATCCCTATTGGCCTCACCAACCAATTGAGAAT
  TTATAA
  
  Clone variation with respect to NM_006356.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006356 unedited
  GTTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACCGTGG
  GCAGCCAGGGTCGGTGAAGGATCCCAAATGGCTGGGCGAAAACTTGCTCTAAAAACCATT
  GACTGGGTAGCTTTTGCACAGATCATACCCCAGAACCAAAAGGCCATTGCTAGTTCCCTG
  AAATCCTGGAATGAGACCCTCACCTCCAGGTTGGCTGCTTTACCTGAGAATCCACCAGCT
  ATCGAAGGGGCTTACTACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGAG
  AACAAGTTTAATGCGCTGAAGGTTCCCGTGCCAGAGGATAAATATACTGCCCACGTGGAT
  GCCGAAGAAAAAGAAGATGTGAAATCTTGTGCTGAGTGGGTGTCTCTCTCAAAGGCCAGG
  ATTGTACAATATGAGAAAGAGATGGAGAAGATGAAGAACTTAATTCCATTTGATCAGATG
  ACCATTGAGGACTTGAATGAAGCTTTCCCAGAAACCAAATTAGACAAGAAAAAGTATCCC
  TATTGGCCTCACCAACCAATTGAGAATTTATAAAATTGACCCCAGGAGGAAGCTCTGGCC
  CTTGTATTACACACTCTGGACATTATAAATAATACTTATACAACACAACAAAAAAAAAAA
  CTAGACTCTAGATTGCGGCCGAGGTCATAGCTGGTTCCTGAACAGATCCCGGGGGGCAAT
  CCTTGTGACCCCTCCCCAAAGCCTTTCCTGCCCCTGAAAATTGCCACTCCAGCGCCCCCC
  CCCCCTGTCCCAAAAAAAAAAGCGCACAATTTCGCCTGACAAGTGGTCCCTCATTATATT
  ATGGGCGGAGGGGGCGGGATTGGACCAAGGGCCAGTTGGGAATACACCCTTAGGCCCGCA
  GGTTTATGGGAACCAACTGGACGCGGGGGCCCATCTCGCCTCCTCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006356 unedited
  CTATGAACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTATAATTATT
  ATTTTTAATGTCCAGAATGTGTAATACAAGGGCCAGAGCTTCCTCCTGGACTCAATTTTA
  TAAATTCTCAATTGGTTGGTGAGGCCAATAGGGATACTTTTTCTTGTCTAATTTGGTTTC
  TGGGAAAGCTTCATTCAAGTCCTCAATGGTCATCTGATCAAATGGAATTAAGTTCTTCAT
  CTTCTCCATCTCTTTCTCATATTCTACAATCCTGGCCTTTGAGAGAGACACCCACTCAGC
  ACAAGATTTCACATCTTCTTTTTCTTCGGCATCCACCTGGGCAGTATATTTATCCTCTGG
  CACGGGAACCTTCAGCGCATTAAACTTCTTCTCAAAGTCATCCACCAAGCCAGCCTTGGC
  CACATTGGCCTTGTAGTAAGCCCAGTCGATAGCTGGTGGATTCTCAGGTAAAGCAGCCAA
  CCTGGAGGTGAGGGTCTCATTCCAGGATTTCAGGGAACTAGCAATGGCCTTTTGGTTCTG
  GGGTATGATCTCTGCAAAAGCTACCCAGTCAATGGTTTTTAGAGCAAGTTTTCGCCCAGC
  CATTTTGGGATCCTTCACCGACCCTGGCTGCCCACGGTCCCCTCGTGCCGAATTCGCGGC
  CGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATA
  TAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATTACGA
  CATTTTGGAAAGGCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGG
  TGGAGACTTGTAAATCCCGGGAGTCAAACCGTTATCACGCCCATTGTGGTCTGCCCAACC
  GCTCACCTGGNATAGCGATACTAATACTCNATGTCCGCCAGTCAGAATCCCCTACTCCTG
  TCCTGGCCTATGCCCGCCGCCTCTTACCGTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006356
• Insert Size: 650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006356.2, NP_006347.1
• RefSeq Size: 628 bp
• RefSeq ORF: 486 bp
• Locus ID: 10476
• UniProt ID: O75947
• Cytogenetics: 17q25.1
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longer transcript, and it encodes the longer isoform a.