MAD2L2 (NM_006341) Human Untagged Clone

CAT#: SC111153

MAD2L2 (untagged)-Human MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAD2L2
• Synonyms: FANCV; MAD2B; POLZ2; REV7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111153 sequence for NM_006341 edited (data generated by NextGen Sequencing)
  ATGACCACGCTCACACGACAAGACCTCAACTTTGGCCAAGTGGTGGCCGATGTGCTCTGC
  GAGTTCCTGGAGGTGGCTGTGCATCTCATCCTCTACGTGCGCGAGGTCTACCCCGTGGGC
  ATCTTCCAGAAACGCAAGAAGTACAACGTGCCGGTCCAGATGTCCTGCCACCCGGAGCTG
  AATCAGTATATCCAGGACACGCTGCACTGCGTCAAGCCACTCCTGGAGAAGAATGATGTG
  GAGAAAGTGGTGGTGGTGATTTTGGATAAAGAGCACCGCCCAGTGGAGAAATTCGTCTTT
  GAGATCACCCAGCCTCCACTGCTGTCCATCAGCTCAGACTCGCTGTTGTCTCATGTGGAG
  CAGCTGCTCCGGGCCTTCATCCTGAAGATCAGCGTGTGCGATGCCGTCCTGGACCACAAC
  CCCCCAGGCTGTACCTTCACAGTCCTGGTGCACACGAGAGAAGCCGCCACTCGCAACATG
  GAGAAGATCCAGGTCATCAAGGATTTCCCCTGGATCCTGGCGGATGAGCAGGATGTCCAC
  ATGCATGACCCCCGGCTGATACCACTAAAAACCATGACGTCGGACATTTTAAAGATGCAG
  CTTTACGTGGAAGAGCGCGCTCATAAAGGCAGCTGA
  
  Clone variation with respect to NM_006341.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006341 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGCGATCGGTC
  CGGTCGGTGGCTCCCCGCGGCGGGGCCGGGCCCGATCTCGGGCGGGAACCGAGCGCAGAG
  CCGGTAGCGGGAAGGATGACCACGCTCACACGACAAGACCTCAACTTTGGCCAAGTGGTG
  GCCGATGTGCTCTGCGAGTTCCTGGAGGTGGCTGTGCATCTCATCCTCTACGTGCGCGAG
  GTCTACCCCGTGGGCATCTTCCAGAAACGCAAGAAGTACAACGTGCCGGTCCAGATGTCC
  TGCCACCCGGAGCTGAATCAGTATATCCAGGACACGCTGCACTGCGTCAAGCCACTCCTG
  GAGAAGAATGATGTGGAGAAAGTGGTGGTGGTGATTTTGGATAAAGAGCACCGCCCAGTG
  GAGAAATTCGTCTTTGAGATCACCCAGCCTCCACTGCTGTCCATCAGCTCAGACTCGCTG
  TTGTCTCATGTGGAGCAGCTGCTCCGGGCCTTCATCCTGAAGATCAGCGTGTGCGATGCC
  GTCCTGGACCACAACCCCCCAGGCTGTACCTTCACAGTCCTGGTGCACACGAGAGAAGCC
  GCCACTCGCAACATGGAGAAGATCCAGGTCATCAAGGATTTCCCCTGGATCCTGGCGGAT
  GAGCAGGATGTCCACATGCATGACCCCCGGCTGATACCACTAAAAACCATGACGTCGGAC
  ATTNTAAAGATGCAGCTNTACGTGGAAGAGCGCGCTCATAAAGGCAGCTGNAGGGGCACC
  TGCCACCCCACTGATGCCCAAACTGTCAGACTTTGNGGNATCCCCGCCTAGGGCAGTGCT
  GCATGGCTGCCCTGATTCCAAGTGCTCTTATCGCCTCTGTGTGTGGATCGCCCGNCCCAG
  CCCGGNGCCGCTCAGTCTGCTTGGAGGATGCTNNCCCAGNAGGCAGTGAGGGATGCCGCA
  CCTCNACTCTCACCTNCTGGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006341 unedited
  GCCGCTTTTAGAGTCGAGNAANTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  AGCCCAGCCCTTGGGGCCCCTTTATTGAAACAACTCACAGCACAGTTTTTGAAACAAACA
  GGGTTGGCCGGGGGAACCCCAGAAGGCTGAAAATTCAAGGTTGCGGCATCCCTCACTGCC
  CTCCTGGGGGAGGCATCCTCCAAGCAAACCTGAGCGGCCCCGGGCTGGGGCGGGCGATCC
  ACACACAGAGGCGATAAAACCACTTGGAATCAGGGCAGCCATGCAGCACTGCCCTAGGCG
  GGGATCCCCCAAAGTCTGACAGTTTGGGCATAAGGGGGGTGGCAGGTGCCCCCTCAGCTG
  CCTTTATGACCGCGCTCTTCCACGTAAAGCTGCATCTTTAAAATGCCCGACGTAATGGTT
  TTTAGGGGTATCAGCCGGGGGTCATGCATGTGGACATCCTGCTCATCCGCCAGGATCCAG
  GGGAAATCCTTGATGACCTGGATCTTCTCCATGTTGCGAGAGGCGGCTTCTCTCGTGTGC
  ACCAGGACTGTGAAGGTACAGCCTGGGGGGTTGTGGCCCAGGACGGCATCGCACACGCTG
  ATCTTAAGGATGAAGGCCCGGACCAGCTGCTCCACATGAGACAACAGCGAGTTCTGATCT
  GATGCACAGCAGTGGAGGCTGGGTGATCCCAAAGACAAATTTCTCCACTGGGCGGAGCTC
  TTTTTCCAAATTACCACCACAATTTTTTCCAATAATCCTTTTCCAGAATTGGTTAGAACA
  ATGCAACGGGTCCTGATATTCTGATTAACCTCCGGTTGAAAGAATTCTGAACCCGGCACC
  TGTCTTCTTGCGTTCTGGAAAATCCCCAGGGGTTACCTCCCCCCCTTAAGGTGAAAAGCA
  CCGCCCCTCCAGAACTCCAAGGACATGCGCCCACTTGCCCAAGTTAAGGCTTGTTGGGAA
  ACGGGCCATCTCCCCGCCACCCTCTCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006341
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006341.2, NP_006332.2
• RefSeq Size: 1056 bp
• RefSeq ORF: 636 bp
• Locus ID: 10459
• UniProt ID: Q9UI95
• Cytogenetics: 1p36.22
• Domains: HORMA
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.