PSMD3 (NM_002809) Human Untagged Clone

CAT#: SC110987

PSMD3 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD3
• Synonyms: P58; RPN3; S3; TSTA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110987 sequence for NM_002809 edited (data generated by NextGen Sequencing)
  ATGAAGCAGGAGGGCTCGGCGCGGCGCCGCGGCGCGGACAAGGCGAAACCGCCGCCCGGC
  GGAGGAGAACAAGAACCCCCACCGCCGCCGGCCCCCCAGGATGTGGAGATGAAAGAGGAG
  GCAGCGACGGGTGGCGGGTCGACGGGGGAGGCAGACGGCAAGACGGCGGCGGCAGCGGCT
  GAGCACTCCCAGCGAGAGCTGGACACAGTCACCTTGGAGGACATCAAGGAGCACGTGAAA
  CAGCTAGAGAAAGCGGTTTCAGGCAAGGAGCCGAGATTCGTGCTGCGGGCCCTGCGGATG
  CTGCCTTCCACATCACGCCGCCTCAACCACTATGTTCTGTATAAGGCTGTGCAGGGCTTC
  TTCACTTCAAATAATGCCACTCGAGACTTTTTGCTCCCCTTCCTGGAAGAGCCCATGGAC
  ACAGAGGCTGATTTACAGTTCCGTCCCCGCACGGGAAAAGCTGCGTCGACACCCCTCCTG
  CCTGAAGTGGAAGCCTATCTCCAACTCCTCGTGGTCATCTTCATGATGAACAGCAAGCGC
  TACAAAGAGGCACAGAAGATCTCTGATGATCTGATGCAGAAGATCAGTACTCAGAACCGC
  CGGGCCCTAGACCTTGTAGCCGCAAAGTGTTACTATTATCACGCCCGGGTCTATGAGTTC
  CTGGACAAGCTGGATGTGGTGCGCAGCTTCTTGCATGCTCGGCTCCGGACAGCTACGCTT
  CGGCATGACGCAGACGGGCAGGCCACCCTGTTGAACCTCCTGCTGCGGAATTACCTACAC
  TACAGCTTGTACGACCAGGCTGAGAAGCTGGTGTCCAAGTCTGTGTTCCCAGAGCAGGCC
  AACAACAATGAGTGGGCCAGGTACCTCTACTACACAGGGCGAATCAAAGCCATCCAGCTG
  GAGTACTCAGAGGCCCGGAGAACGATGACCAACGCCCTTCGCAAGGCCCCTCAGCACACA
  GCTGTCGGCTTCAAACAGACGGTGCACAAGCTTCTCATCGTGGTGGAGCTGTTGCTGGGG
  GAGATCCCTGACCGGCTGCAGTTCCGCCAGCCCTCCCTCAAGCGCTCACTCATGCCCTAT
  TTCCTTCTGACTCAAGCTGTCAGGACAGGAAACCTAGCCAAGTTCAACCAGGTCCTGGAT
  CAGTTTGGGGAGAAGTTTCAAGCAGATGGGACCTACACCCTAATTATCCGGCTGCGGCAC
  AACGTGATTAAGACAGGTGTACGCATGATCAGCCTCTCCTATTCCCGAATCTCCTTGGCT
  GACATCGCCCAGAAGCTGCAGTTGGATAGCCCCGAAGATGCAGAGTTCATTGTTGCCAAG
  GCCATCCGGGATGGTGTCATTGAGGCCAGCATCAACCACGAGAAGGGCTATGTCCAATCC
  AAGGAGATGATTGACATCTATTCCACCCGAGAGCCCCAGCTAGCCTTCCACCAGCGCATC
  TCCTTCTGCCTAGATATCCACAACATGTCTGTCAAGGCCATGAGGTTTCCTCCCAAATCG
  TACAACAAGGACTTGGAGTCTGCAGAGGAACGGCGTGAGCGAGAACAGCAGGACTTGGAG
  TTTGCCAAGGAGATGGCAGAAGATGATGATGACAGCTTCCCTTGA
  
  Clone variation with respect to NM_002809.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002809 unedited
  GGTACGATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCCGGCTCGGC
  TCCTGGTCCCCGGTGCGAGGGTTAACGCGAGGCCCCGGCCTCGGTCCCCGGACTAGGCCG
  TGACCCCGGGTGCCATGAAGCAGGAGGGCTCGGCGCGGCGCCGCGGCGCGGACAAGGCGA
  AACCGCCGCCCGGCGGAGGAGAACAAGAACCCCCACCGCCGCCGGCCCCCCAGGATGTGG
  AGATGAAAGAGGAGGCAGCGACGGGTGGCGGGTCGACGGGGGAGGCAGACGGCAAGACGG
  CGGCGGCAGCGGCTGAGCACTCCCAGCGAGAGCTGGACACAGTCACCTTGGAGGACATCA
  AGGAGCACGTGAAACAGCTAGAGAAAGCGGTTTCAGGCAAGGAGCCGAGATTCGTGCTGC
  GGGCCCTGCGGATGCTGCCTTCCACATCACGCCGCCTCAACCACTATGTTCTGTATAAGG
  CTGTGCAGGGCTTCTTCACTTCAAATAATGCCACTCGAGACTTTTTGCTCCCCTTCCTGG
  AAGAGCCCATGGACACAGAGGCTGATTTACAGTTCCGTCCCCGCACGGGAAAAGCTGCGT
  CGACACCCCTCCTGCCTGAAGTGGAAGCCTATCTCCAACTCCTCGTGGTCATCTTCATGA
  TGAACAGNCAGCGCTACANAGAGGCACAGAAGATCTCTGATGATCTGATGCAGAAGATCA
  GTACTCAGAACCGCCGGGCCCTAGACCTTGTAGCCGCAAAGTGTTACTATTATCACGCCC
  CGGGTCTATGAGTTCCCTGGGACAGCTGGATGTGGTGCGCAGCTTCTTGCATGCTCGGTT
  CCGGACAGTTACGCTTCGCATGACCCAAACGGGCAGGCCACCTTGTTGACCTCTGCTGCG
  AATTACTACACTCAACTGGACGACCAGCTGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002809 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGGAGAACTTTTTATTGTTTA
  AAAATCATAATCGAAGCTTCAAAAAACATACAAGCCAAGACATCCCAGTTCTAGTCCCCC
  ACTCCCTAAAGGAGTACACAGAACTATCCTTCCCCTCCTGCTCCCACACTGCTGGCCAGG
  ACCCTGCCCGGGGAGGTTGCCTGCCCACCCTCCTGCCTGCTGTACAGTAAGTCACCGGCT
  GGGGAGGAGCCCTGGGGGAGGTCAGGGTGGCTGGCTCCCAGCACCCCCACCCCCTGCACG
  AATGCAGCATATGAGCTGTGTGTGGGGAAAATGGGGACAGTGCCCTGGGCAGGGGACCCC
  CAAGGGGGAAAGTGCCTGTCCCCATTCCCCCTACCCCTCCCCAGCCCCCCAGCTCAAGGG
  AAGCTGCCATCATCATCTTCTGCCATCTCCTTGGCAAACTCCAAGTTCTGCTGTTCTCGC
  TCACCCCGTTCCTCTGCAGACTCCAAGTCCTTGTTGTACGATTTGGGAGGAAACCTCATG
  GCCTTGACAGACATGTTGCGGATATCTAGGCACAATGAGATGCCCTGGTGGAGGCTACCT
  GGGGCTCCTCGGCTGGAATAGAAGCCATCATCTCCTTGGATCGACATACCCCTTCTCGGG
  TTGATGCTGGGCTCATTGACACATCCCGGAGGCCCTTGCCACCATGAACTTGCATCTTCG
  GGGCTTTCCACCTGCAGCTTTCGGGCGAAGTCACCCAAGAACATCCGGAAAGGACACGCT
  GATCATGCGCCACCGGCTTATACACGTGGGCCCGAACCCGATAATTATGGCGAAGGCCCA
  CTTGTTGGAACCTCTCCCCACCTAACCAGAACCTGGTGACACTGCTAAGGTCCCTCCCCG
  CCAGCTTGATACACAGGAATAGCCCCCATTATCCCCTTGTGGAAGGCGGGTGAATTGCAC
  TGCCAAGAACCCCCACAAAACTCTCCACCAAAAAAACTTCGCCCCTCTTTTAACCCCACA
  CCGCTCTGAGGCCCTTCAAGGCTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002809
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002809.2, NP_002800.2
• RefSeq Size: 2174 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5709
• UniProt ID: O43242
• Cytogenetics: 17q21.1
• Domains: PCI
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]