hemoglobin subunit gamma 1 (HBG1) (NM_000559) Human Untagged Clone

CAT#: SC110916

HBG1 (untagged)-Human hemoglobin, gamma A (HBG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hemoglobin subunit gamma 1
• Synonyms: HBG-T2; HBGA; HBGR; HSGGL1; PRO2979
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000559, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG
  CTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGG
  CAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACT
  TCCTTGGGAGATGCCACAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACT
  GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAAT
  CCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGT
  GCCCTGTCCTCCAGATACCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000559 unedited
  GGGTCCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGAACGTCT
  GAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGG
  CTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGG
  GAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGT
  CCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGA
  CTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGA
  GTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATG
  TGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTT
  CCTGGCAGAAGATGGTGACTGGAGTGGCCAGTGCCCTGTCCTCCAGATACCACTGAGCTC
  ACTGCCCATGATGCAGAGCTTTCAAGGATAGGCTTTATTCTGCAAGCAATCAAATAATAA
  ATCTATTCTGCTAAGAGATCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
  GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC
  TCTCCTGGCCCTGGAAAGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAG
  TTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATATGGGGTGAGGGGGTGGGGTT
  TGGACCAGGGGCCAATTGGAAAACAACCCGAAGGCCCCCGGGTCTATGGGAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000559 unedited
  NNAAGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATCT
  CTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTC
  TGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCA
  CCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCA
  AAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGT
  CACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGG
  CATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGA
  TGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTAGA
  CAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACA
  GGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCTGGACTAGGAG
  CTTATTGATAACCTCAGACGTTCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCG
  TATTACAAAAATCTGACGGTTCACCTAACGAGCTCTGCTTATATAGACCTCCCACCGTAC
  ACGCCTACCGGCCATTTGGGGCAACGGGGCGGGGTTATACCACATTTTGGAAAGTCCCCG
  TGATTTTGGGGCCAAAACAACTCCCATTGACGCAATGGGGCGGAGCTTTGGAAATCCCCG
  GAGGCAAACCCGCTCTCCCGCCCTGGGCGGACCGCCCAACCCCCCTCCCCTGGTAAAAGC
  AGGCCTATACCGCATGGCCGCCCACCGCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000559
• Insert Size: 444 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000559.2, NP_000550.2
• RefSeq Size: 584 bp
• RefSeq ORF: 444 bp
• Locus ID: 3047
• UniProt ID: P69891
• Cytogenetics: 11p15.4
• Domains: globin
• Gene Summary: The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]