TFIIB (GTF2B) (NM_001514) Human Untagged Clone

CAT#: SC110914

GTF2B (untagged)-Human general transcription factor IIB (GTF2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFIIB
• Synonyms: TF2B; TFIIB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001514, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAG
  TGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGA
  TGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
  CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTG
  ACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGC
  ATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
  TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTC
  TCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTC
  TAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
  ACTGGGGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACAC
  ATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGC
  TATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
  GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT
  TCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001514 unedited
  CGTTTACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGTTGTGT
  CTTGTTGCGGGCACCGCAGTCGCCGTGAAGATGGCGTCTACCAGCCGTTTGGATGCTCTT
  CCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAGTGGAGGACTACAGAGCCGGT
  GATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGATGTGGGATCT
  GAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
  CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCT
  GCAAGTTTTGACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCT
  GATCGGGCAATGATGAATGCATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTA
  CCTCGAAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTATATGAACAGAAGAGC
  CTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAA
  GAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTCTAAGAAAGAA
  ATTGGTCGGTGGTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
  ACTGGGGACTTCATGTCCAGGTTCTGGTCCAACCTTTGTCTTCCTNNAACAGTACAGATG
  GCAGCTACACATATAGCCCCGTAAGCTGTGGAATTGGACTTGGTTCCTGGGNNAGAGCCC
  CATCTCTGTGGCAGCGGCAGCTATTTACATGGCCTCACAGCCATCAGCTGGAAAGAGGAC
  CCACAAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001514 unedited
  TGTACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTTTGAAAAA
  TGTTTTATTCAAGATTACATAATTTCAAATATCTTCCAAAATACAGTTTCCTAGATTGCT
  ACAAAAGAAATTTGATAAGAAATTTAAATCATTAAATATGTTTCACTAGTATATACATAC
  AGAATGCCAAGCAATAGTATTCAGCCCTGGAATGCGTACCATGTCTTTTGTTTTTTCTCA
  TGAAAGGCTCAACCCAGCATTTTGCATAGGCTATGTACAACAGGCAAAGTTTTGTATTCA
  AGAATTTGACGTACGCTGCCTCAATTTATAGCCTGTGGCACTTCGCCCACTGGGGTGTCA
  CATTTGAAGCCTGTAGGAACCAGATTTGGGGCTCGAGGATAGACCAGTCTATAGGACTGT
  CTGATTGTACCATCAGCAACACCAGCATTCTCTCCAATTTCCTTCTGCGTCCTCTCTTAC
  CTGATGCCCGCGAGGCCATGCCAATAGCTGTCGTTGCCACAGACACGGCCGCTCCTCCTT
  ATGAGTCCCGTCCCCCTCACAGCCTTACGGGCTACATGTGTAGCTGCCATCCGCCCACGG
  CTAGGAAGACAAAGCGTCGCCACCGAACCTGGACACGAATTCCCTCAGTTGTCATTCACA
  CCCACATTGGTTCCTAGCGCCTTTCAACTCAGTTCCAACCACCGCCCCCCCCCTCTCTAT
  CAACCCGGCCCCGGCACATATTTTTTTCACCGCCCTAAGACCCCCCCCCCTGCCTTAAGC
  CATATACTATTCTTTTCCCTCCTTTTTCCCCACCACCCCCTCCCCTCCAGGCTTCTCTTG
  GAACCAAACTTGTTTCGCACCCATATCCGTTCGCCAACCTCTCTCGCGACGGTTCATGCT
  CTCCCCCGCCCGGCAGGTGACTATTTGAAGCACTTATCACTCGCCACAGCACCGCCCCGT
  TTTCACACCGCCCTCTAACTGCCCTCCCGTAACCTGGGCCCCGCTTTTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001514
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001514.3, NP_001505.1
• RefSeq Size: 1304 bp
• RefSeq ORF: 951 bp
• Locus ID: 2959
• UniProt ID: Q00403
• Cytogenetics: 1p22.2
• Domains: transcript_fac2, CYCLIN
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]