Aminopeptidase A (ENPEP) (NM_001977) Human Untagged Clone

CAT#: SC110882

ENPEP (untagged)-Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aminopeptidase A
• Synonyms: APA; CD249; gp160
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110882 sequence for NM_001977 edited (data generated by NextGen Sequencing)
  ATGAACTTTGCGGAGAGAGAGGGCTCTAAGAGATACTGCATTCAAACGAAACATGTGGCC
  ATTCTCTGTGCGGTGGTGGTGGGTGTAGGATTAATAGTGGGACTTGCCGTGGGCTTGACC
  AGATCGTGTGACTCCAGCGGGGACGGCGGGCCGGGCACTGCGCCAGCTCCTTCCCACCTG
  CCTTCTTCCACGGCCAGCCCCTCAGGTCCTCCTGCCCAGGACCAGGACATCTGCCCGGCC
  AGTGAGGATGAGAGCGGACAGTGGAAAAACTTTCGACTGCCGGACTTCGTCAACCCAGTC
  CACTACGACCTGCACGTGAAGCCCCTGTTGGAGGAGGACACCTACACGGGCACCGTGAGC
  ATCTCCATCAACCTGAGCGCTCCCACCCGGTACCTGTGGCTGCACCTCCGGGAGACCAGG
  ATCACCCGGCTCCCGGAGCTGAAGAGGCCCTCTGGGGACCAGGTGCAAGTCCGGAGGTGT
  TTCGAGTACAAAAAGCAGGAGTACGTGGTGGTCGAGGCGGAGGAAGAGCTTACCCCCAGC
  AGTGGAGATGGCCTGTATCTCCTGACCATGGAGTTCGCCGGCTGGCTGAACGGCTCCCTC
  GTGGGATTTTATAGAACCACCTACACGGAGAACGGACAAGTCAAGAGCATAGCGGCCACC
  GATCATGAACCAACAGATGCCAGGAAATCTTTTCCTTGTTTTGATGAGCCCAACAAAAAG
  GCAACTTATACAATATCTATCACCCATCCCAAAGAATACGGAGCACTTTCAAATATGCCA
  GTGGCGAAAGAAGAGTCAGTGGATGATAAATGGACTCGAACAACTTTTGAGAAGTCTGTC
  CCCATGAGCACGTACCTGGTGTGCTTTGCTGTACATCAATTTGACTCTGTAAAGAGAATA
  TCAAATAGTGGAAAACCTCTTACAATTTATGTCCAGCCAGAGCAAAAGCACACAGCCGAA
  TATGCTGCAAACATAACTAAAAGTGTGTTTGATTATTTTGAAGAATACTTTGCTATGAAT
  TATTCTCTTCCTAAATTAGATAAAATCGCTATTCCAGATTTTGGCACTGGTGCCATGGAG
  AACTGGGGACTCATCACGTACAGAGAAACGAACCTGCTTTATGACCCTAAGGAATCAGCC
  TCATCAAACCAACAGAGGGTGGCCACTGTGGTTGCCCATGAACTTGTGCATCAGTGGTTT
  GGAAATATTGTGACCATGGACTGGTGGGAAGACTTGTGGCTAAATGAAGGATTTGCTTCT
  TTCTTTGAGTTTCTGGGAGTAAACCATGCAGAAACAGACTGGCAAATGCGTGACCAAATG
  TTACTTGAAGATGTATTACCTGTTCAAGAGGATGATTCTTTGATGTCTTCGCATCCAATT
  ATTGTGACTGTGACAACCCCTGATGAAATAACATCTGTTTTTGATGGAATATCCTATAGC
  AAGGGATCTTCTATTTTGAGAATGCTTGAAGACTGGATAAAACCAGAGAATTTTCAAAAA
  GGATGTCAGATGTACTTGGAAAAATACCAATTCAAGAATGCAAAAACTTCTGATTTTTGG
  GCAGCACTGGAAGAGGCAAGTAGGCTACCAGTGAAAGAAGTAATGGACACCTGGACCAGA
  CAGATGGGTTATCCTGTGCTTAACGTGAACGGTGTCAAGAACATCACACAGAAACGCTTT
  TTGTTGGACCCAAGAGCTAACCCTTCTCAGCCCCCTTCAGATCTTGGTTATACATGGAAT
  ATCCCAGTTAAATGGACTGAAGATAATATAACAAGCAGTGTGTTATTTAATAGGTCAGAA
  AAAGAAGGAATCACTTTGAACTCCTCTAATCCTAGTGGAAATGCTTTTCTCAAAATAAAC
  CCAGATCATATTGGGTTTTATCGTGTAAATTATGAAGTAGCAACTTGGGACTCGATAGCT
  ACAGCGCTCTCCTTGAACCACAAGACATTTTCTTCAGCAGATCGTGCAAGTCTTATTGAT
  GATGCTTTTGCCTTGGCAAGAGCTCAACTTCTAGATTATAAGGTGGCTTTGAACTTGACC
  AAGTATCTCAAAAGGGAAGAGAATTTTTTACCATGGCAGAGAGTAATTTCAGCTGTAACC
  TACATCATTAGCATGTTTGAAGATGATAAAGAGCTATATCCTATGATTGAGGAATACTTC
  CAAGGTCAAGTGAAGCCTATTGCAGATTCTCTGGGATGGAATGATGCTGGAGACCATGTC
  ACAAAGTTACTCCGTTCCTCCGTGTTAGGGTTTGCGTGCAAGATGGGAGACAGAGAAGCC
  TTGAACAATGCTTCCTCGTTATTTGAGCAGTGGCTAAATGGGACTGTAAGCCTTCCCGTA
  AATCTCAGGCTTCTGGTGTATCGGTATGGGATGCAGAACTCTGGCAATGAGATTTCATGG
  AACTACACTCTTGAGCAATACCAGAAAACTTCATTAGCTCAAGAAAAAGAAAAACTGCTG
  TATGGATTAGCATCAGTGAAGAACGTTACTCTTTTGTCAAGGTATTTGGATTTGCTCAAG
  GACACGAACCTTATTAAAACTCAGGATGTGTTTACAGTCATTCGATATATCTCATATAAC
  AGCTATGGGAAGAACATGGCCTGGAATTGGATACAACTCAACTGGGACTATCTAGTCAAC
  AGATATACACTCAATAACAGAAACCTTGGCCGAATTGTCACAATAGCAGAGCCATTCAAC
  ACTGAACTGCAACTGTGGCAGATGGAGAGCTTTTTTGCAAAATATCCACAAGCTGGAGCA
  GGAGAAAAACCTAGGGAACAAGTGCTGGAAACAGTGAAAAACAATATAGAGTGGCTAAAA
  CAACATAGAAACACCATCAGAGAATGGTTTTTTAATTTACTTGAGAGTGGTTAA
  
  Clone variation with respect to NM_001977.3
  653 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001977 unedited
  GGTGCGATTTTGTAATACGATTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTCCT
  CCCACTCGGCTCCTCTTACGGAGTCCTCATTCCACCCCCCTTGTTTCCGCATTCATCCTG
  AGTGGCTGGTGGGAACGTGAAAAGGGAGAGGAAAAGGCGCAAGAAGCCAGAGAGAGGGGT
  GTGGGAAAAGCGAAAACAGGCTGCCAAATCAGGGGATTCCTTCCAATTTAAAAAGGAAGT
  CTGCTGACGTTAGTTAGTTAAATTTAACATCTTTTTATGTGTAACACTTGACTTTGGAAG
  CAAAAATGAACTTTGCGGAGAGAGAGGGCTCTAAGAGATACTGCATTCAAACGAAACATG
  TGGCCATTCTCTGTGCGGTGGTGGTGGGTGTAGGATTAATAGTGGGACTTGCCGTGGGCT
  TGACCAGATCGTGTGACTCCAGCGGGGACGGCGGGCCGGGCACTGCGCCAGCTCCTTCCC
  ACCTGCCTTCTTCCACGGCCAGCCCCTCAGGTCCTCCTGCCCAGGACCAGGACATCTGCC
  CGGCCAGTGAGGATGAGAGCGGACAGTGGAAAAACTTTCGACTGCCGGACTTCGTCAACC
  CAGTCCACTACGACCTGCACGTGAAGCCCCTGTTGGAGGAGGACACCTACACGGGCACCG
  TGAGCATCTCCATCAACCTGAGCGCTCCCACCCGGTACCTGTGGCTGCACCTCCGGGAGA
  CCAGGATCACCCGGCTCCCGGAGCTGAAGAGGCCCTCTTGGGACCAGGTGNCAGTCCGGA
  GGTGTTTCGAGTACAAAAGCAAGAGTACGTTGTTGTCCGAGCGGGAGGAGAACTTTACCC
  CCACAGGGGAGAGGGCCTGTATCTCCTGACCATGNAGATCCGCCGCCTGGTTGAACGGCT
  CCCTCGTGGGATTTATTGAACCACCTACCCGAGAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001977 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCAAGAAATCATTTAGATTTAT
  TTTTAAAAAAAGAGGAGAGTTCTCTTATTGATTTTATAAGAGCAAAGGCAACAGTCAGCC
  ATTCTTTTGGCTAATTCATATGATCCTCAAGTAAAACTTAAGCCCAGGTTACTTTGGTAG
  GATTTAAAGTAGTAAGACATATGTGAATAAAATGATATTAGGTCTGTATAATTCTGAATA
  ATAAAATAAAGATTAAGATGGTAATATTGGTCCCTCAAGTACTGGTATTTAAACTTTAAA
  AATCAATCAAGAGAGAAGATTAAGTGGTGTTTCTTTGGTACTGAAAATGTAAAGCTAAGA
  CCAAATTGTTCTACTTCACAGATTTAAGATACAAAGAATGATATTTAAAGGTATTAAAAT
  AACTATTTCATTATCTTCAGTTTGTAAAGAATATCTTATGAAGTACACTTAGAAGGAATC
  CCTGAGTATATTAAGTACTCTATTAGTATCTTTCTTCATAAACAAAGAGGAACAATTTAA
  AGGCTTTGCAAGACATATAAACACAGTGCTTAGTAAAAGCATTATCTGTTTATAAGGCTC
  TCTCCATCGTGCTTGTAAATTAGGCCACCAAATGCTCCAGAGGAGAAACAATAGATCCAC
  ACAATTAAACTCTAACATTTGAATACATTAACCACTCTCAAGTAAATTAAAAAACCATTC
  TCTGATGGCGCTTCTATGTTGCTNTAGCCACCCTATATTGTTTTTCACTGCTTCCAGCAC
  TTGTTCCCTAGGTCTTTCTCCTGGTCCACCTCGGGNATATCTTCGCACAAAGCTCCTCAT
  CTGCCCAGCCGAGTTTCGTGTCGCATGGCTTCGTTTTGGGACAATCGGCCCCGGTTCCGC
  TCTCGACGTACTCTGGCGCCCCAAACCCCAGCGCCCTGTTTCACACCCAGCCCGGCCTTT
  TATTACTGCCCCCCAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001977
• Insert Size: 4020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001977.2, NP_001968.2
• RefSeq Size: 3829 bp
• RefSeq ORF: 2874 bp
• Locus ID: 2028
• UniProt ID: Q07075
• Cytogenetics: 4q25
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Renin-angiotensin system
• Gene Summary: The ENPEP gene encodes glutamyl aminopeptidase, a type II integral membrane protein with an extracellular zinc-binding domain. This protein can upregulate blood pressure by cleaving the N-terminal aspartate from angiotensin II, and can regulate blood vessel formation and enhance tumorigenesis in some tissues. Along with ANPEP and DPP4, ENPEP was found to be a candidate co-receptor for the coronavirus SARS-CoV-2, which causes COVID-19. [provided by RefSeq, Apr 2020]