CARD9 (NM_052813) Human Untagged Clone

CAT#: SC110604

CARD9 (untagged)-Human caspase recruitment domain family, member 9 (CARD9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARD9
• Synonyms: CANDF2; hCARD9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_052813 edited
  GAATTCGGCACGAGCCGGCGTGCTCCTCCCTCCCTGCAGCCCCGGGCAGCATCTCCCAGA
  GGCTCCGCGGCCCAGGCTCCTGGTGTGTCTGCAGTGCAGGTGGCTCCTGGAAGACCCTCA
  GCCTGCCTGCTGAGGCCATGTCGGACTACGAGAACGATGACGAGTGCTGGAACGTCCTGG
  AGGGCTTCCGGGTGACGCTCACCTCGGTCATCGACCCCTCACGCATCACACCTTACCTGC
  GGCAGTGCAAGGTCCTGAACCCTGATGATGAGGAGCAGGTGCTCAGCGACCCCAACCTGG
  TCATCCGCAAACGGAAAGTGGGTGTGCTCCTGGACATCCTGCAGCGGACCGGCCACAAGG
  GCTACGTGGCCTTCCTCGAGAGCCTGGAGCTCTACTACCCGCAGCTGTACAAGAAGGTCA
  CAGGCAAGGAGCCGGCCCGCGTCTTCTCCATGATCATCGACGCGTCCGGGGAGTCAGGCC
  TGACTCAGCTGCTGATGACTGAGGTCATGAAGCTGCAGAAGAAGGTGCAGGACCTGACCG
  CGCTGCTGAGCTCCAAAGATGACTTCATCAAGGAGCTGCGGGTGAAGGACAGCCTGCTGC
  GCAAGCACCAGGAGCGTGTGCAGAGGCTCAAGGAGGAGTGCGAGGCCGGCAGCCGCGAGC
  TCAAGCGCTGCAAGGAGGAGAACTACGACCTGGCCATGCGCCTGGCGCACCAGAGTGAGG
  AGAAGGGCGCCGCGCTCATGCGGAACCGTGACCTGCAGCTGGAGATTGACCAGCTCAAGC
  ACAGCCTCATGAAGGCCGAGGACGACTGCAAGGTGGAGCGCAAGCACACGCTGAAGCTCA
  GGCACGCCATGGAGCAGCGGCCCAGCCAGGAGCTGCTGTGGGAGCTGCAGCAGGAGAAGG
  CCCTGCTCCAGGCCCGGGTGCAGGAGCTGGAGGCCTCCGTCCAGGAGGGGAAGCTGGACA
  GGAGCAGCCCCTACATCCAGGTACTGGAGGAGGACTGGCGGCAGGCGCTGCGGGACCACC
  AGGAGCAGGCCAACACCATCTTCTCCCTGCGCAAGGACCTCCGCCAGGGCGAGGCCCGAC
  GCCTCCGGTGCATGGAGGAGAAGGAGATGTTCGAGCTGCAGTGCCTGGCACTACGTAAGG
  ACTCCAAGATGTACAAGGACCGCATCGAGGCCATCCTGCTGCAGATGGAGGAGGTCGCCA
  TTGAGCGGGACCAGGCCATAGCCACGCGGGAGGAGCTGCACGCACAGCACGCCCGGGGCC
  TGCAGGAGAAGGACGCGCTGCGCAAGCAGGTGCGGGAGCTGGGCGAGAAGGCGGATGAGC
  TGCAGCTGCAGGTGTTCCAGTGTGAGGCGCAGCTACTGGCCGTGGAGGGCAGGCTCAGGC
  GGCAGCAGCTGGAGACGCTCGTCCTGAGCTCCGACCTGGAAGATGGCTCACCCAGGAGGT
  CCCAGGAGCTCTCACTCCCCCAGGACCTGGAGGACACCCAGCTCTCAGACAAAGGCTGCC
  TTGCCGGCGGGGGGAGCCCGAAACAGCCCTTTGCAGCTCTGCACCAGGAGCAGGTTTTGC
  GGAACCCCCATGACGCAGGCCTGAGCAGCGGGGAGCCGCCCGAGAAGGAGCGGCGGCGCC
  TCAAAGAGAGTTTTGAGAACTACCGCAGGAAGCGCGCCCTCAGGAAGATGCAGAAAGGAT
  GGCGGCAGGGGGAGGAGGACCGGGAGAACACCACGGGCAGCGACAACACCGACACTGAGG
  GCTCCTAGCCGCAGCAGCGCAGGCCCCGACCAGGGCACACCCACCGGCCCGGCCTCCTGC
  CACCCGGGGGTGCCGACGCCCTGGGGCGCAGACTTCCCCGAGCCGTCGCTGACTTGGCCT
  GGAACGAGGAATCTGGTGCCCTGAAAGGCCCAGCCGGACTGCCGGGCATTGGGGCCGTTT
  GTTAAGCGGCACTCATTTTGCGGAGGCCATGCGGGTGCTCACCACCCCCATGCACACGCC
  ATCTGTGTAACTTCAGGATCTGTTCTGTTTCACCATGTAACACACAATACATGCATGCAC
  TGTATTAGTGTTAGAAAACACAGCTGCGTAAATAAACAGCACGGGTGACCCGCAAAAAAA
  AAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052813 unedited
  TGCAGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGGCGT
  GCTCCTCCCTCCCTGCAGCCCCGGGCAGCATCTCCCAGAGGCTCCGCGGCCCAGGCTCCT
  GGTGTGTCTGCAGTGCAGGTGGCTCCTGGAAGACCCTCAGCCTGCCTGCTGAGGCCATGT
  CGGACTACGAGAACGATGACGAGTGCTGGAACGTCCTGGAGGGCTTCCGGGTGACGCTCA
  CCTCGGTCATCGACCCCTCACGCATCACACCTTACCTGCGGCAGTGCAAGGTCCTGAACC
  CTGATGATGAGGAGCAGGTGCTCAGCGACCCCAACCTGGTCATCCGCAAACGGAAAGTGG
  GTGTGCTCCTGGACATCCTGCAGCGGACCGGCCACAAGGGCTACGTGGCCTTCCTCGAGA
  GCCTGGAGCTCTACTACCCGCAGCTGTACAAGAAGGTCACAGGCAAGGAGCCGGCCCGCG
  TCTTCTCCATGATCATCGACGCGTCCGGGGAGTTAGGCCTGACTCAGCTGCTGATGACTG
  AGGTCATGAAGCTGCAGAAGAAAGTGCAGGACCTGACCGCGCTGCTGAGCTCCAAAGATG
  ACTTCATCAAGGAGCTGCGGGTGAAGGACAGCCTGCTGCGCAAGCACCAGGAGCGTGTGC
  AGAGGCTCATTGAGGAGTGCGAGGCCCGCAGCCGCGAGCTCAGCGCCTCAAGGAGGAAAA
  CTACGACCTGGCCATGCGCCTGGCGCACCATAATGAAGAAAAAGGGCGCCGCGCCTATTG
  CGGAACCGTGACCTGCAGCTGGAGATTGACCAGCTCAAGCCCAGCCTCTTAAAGGCCGAG
  ACGACTGCAAGTTGGAACGCAAGCCACCCTTGAACTAAGCCCCCNTGGAGCACGGGCCAA
  CCAGAAACTGTTGGGGAGCTTCACAAGAAAAGCCTGTTCCAGCCCGNG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052813 unedited
  AAAACTAGGACCGCGGCCCCACACCTANGATCGATTTATATATCTTTTTTTTTTTTTTGC
  GGGTCACCCGTGCTGTTTATTTACGCAGCTGTGTTTTCTAACACTAATACAGTGCATGCA
  TGTATTGTGTGTTACATGGTGAAACAGAACAGATCCTGAAGTTACACAGATGGCGTGTGC
  ATGGGGGTGGTGAGCACCCGCATGGCCTCCGCAAAATGAGTGCCGCTTAACAAACGGCCC
  CAATGCCCGGCAGTCCGGCTGGGCCTTTCAAGGCACCAGATTCCTCGTTCCAGGCCAAGT
  CAGCGACGGCTCGGGGAAGTCTGCGCCCCAAGGCGTCGGCACCCCCGGGTGGCAAGAAGC
  CGGGCCGGTGGGTGTGCCCTGGTCGGGGCCTGCGCTGCTGCGGCTAGGAGCCCTCAGTGT
  CGGTGTTGTCGCTGCCCGTGGTGTTCTCCCGGTCCTCCTCCCCCTGCCGCCATCCTTTCT
  GCATCTTCCTGAAGGCGCGCTTCCTGCGGTAGTTCTCAAAACTCTCTTTGAGGCGCCGCC
  GCTCCTTCTCGGGCGGCTCCCCGCTGCTCAAGCCTGCGTCATGGGGGTTCCGCAAAACCT
  GCTCCTGGTGCAAAGCTGCAAAGGGCTGTTTCGGGCTCCCCCCGCCGGCAAAGCAACCTT
  TGTCTGAGAGCTGGGTGTCCTCCAAGTCCTGGGGGAGTGAGAGCTCCTGGGACCTCCTGG
  GTGAGCCATCTTCCAAGTCGGAGCTCAAGACGAGCGTCTCCAGCTGCTGGCGCCTGAGCC
  TGCCCTCCCGGCCAGTAGCTGCGGCCTCAACTGGGACACCTGCAGCTGCAGCTTATACCG
  CCTTTTGGCCAATTTCCGGACTGGTTGACCAAAGCGTCCTTTTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_052813
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052813.2, NP_434700.2
• RefSeq Size: 2136 bp
• RefSeq ORF: 1611 bp
• Locus ID: 64170
• UniProt ID: Q9H257
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).