HSD3B7 (NM_025193) Human Untagged Clone

CAT#: SC110313

HSD3B7 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD3B7
• Synonyms: CBAS1; PFIC4; SDR11E3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110313 sequence for NM_025193 edited (data generated by NextGen Sequencing)
  ATGGCCGACTCTGCACAGGCCCAGAAGCTGGTGTACCTGGTCACAGGGGGCTGTGGCTTC
  CTGGGAGAGCACGTGGTGCGAATGCTGCTGCAGCGGGAGCCCCGGCTCGGGGAGCTGCGG
  GTCTTTGACCAACACCTGGGTCCCTGGCTGGAGGAGCTGAAGACAGGGCCTGTGAGGGTG
  ACTGCCATCCAGGGGGACGTGACCCAGGCCCATGAGGTGGCAGCAGCTGTGGCCGGAGCC
  CATGTGGTCATCCACACGGCTGGGCTGGTAGACGTGTTTGGCAGGGCCAGTCCCAAGACC
  ATCCATGAGGTCAACGTGCAGGGTACCCGGAACGTGATCGAGGCTTGTGTGCAGACCGGA
  ACACGGTTCCTGGTCTACACCAGCAGCATGGAAGTTGTGGGGCCTAACACCAAAGGTCAC
  CCCTTCTACAGGGGCAACGAAGACACCCCATACGAAGCAGTGCACAGGCACCCCTATCCT
  TGCAGCAAGGCCCTGGCCGAGTGGCTGGTCCTGGAGGCCAACGGGAGGAAGGTCCGTGGG
  GGGCTGCCCCTGGTGACGTGTGCCCTTCGTCCCACGGGCATCTACGGTGAAGGCCACCAG
  ATCATGAGGGACTTCTACCGCCAGGGCCTGCGCCTGGGAGGTTGGCTCTTCCGGGCCATC
  CCGGCCTCTGTGGAGCATGGCCGGGTCTATGTGGGCAATGTTGCCTGGATGCACGTGCTG
  GCAGCCCGGGAGCTGGAGCAGCGGGCAGCCCTGATGGGCGGCCAGGTATACTTCTGCTAC
  GATGGATCACCCTACAGGAGCTACGAGGATTTCAACATGGAGTTCCTGGGCCCCTGCGGA
  CTGCGGCTGGTGGGCGCCCGCCCATTGCTGCCCTACTGGCTGCTGGTGTTCCTGGCTGCC
  CTCAATGCCCTGCTGCAGTGGCTGCTGCGGCCACTGGTGCTCTACGCACCCCTGCTGAAC
  CCCTACACGCTGGCCGTGGCCAACACCACCTTCACCGTCAGCACCGACAAGGCTCAGCGC
  CATTTCGGCTATGAGCCCCTGTTCTCGTGGGAGGATAGCCGGACCCGCACCATTCTCTGG
  GTACAGGCCGCTACGGGTTCAGCCCAGTGA
  
  Clone variation with respect to NM_025193.3
  748 a=>g;1068 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025193 unedited
  ATTATGTATACGACTCACTATAGGGCGGCNCGCGAATCGGCACCAGGAGGCCCCTCCAGG
  CCAGTCTGGGCACCCTGGGATAGCGGCTGCAGCCATCAGCAGGGGCAGACGGCAGGTGGC
  CTGGTTGCTGCAGCTCCCAGGATCAGCTCTGCCCTCCCGCCAAACGCCAGCCTCGTCACC
  GCTCCAGGGCACCTCCAGCAGTAACAGGTGGTTGCAGCAGGTGGCAGCCAGCCCCTGGAT
  GAGCCAAGGTCTCTTCCCCAGCCAGGCATGGCCGACTCTGCACAGGCCCAGAAGCTGGTG
  TACCTGGTCACAGGGGGCTGTGGCTTCCTGGGAGAGCACGTGGTGCGAATGCTGCTGCAG
  CGGGAGCCCCGGCTCGGGGAGCTGCGGGTCTTTGACCAACACCTGGGTCCCTGGCTGGAG
  GAGCTGAAGACAGGGCCTGTGAGGGTGACTGCCATCCAGGGGGACGTGACCCAGGCCCAT
  GAGGTGGCAGCAGCTGTGGCCGGAGCCCATGTGGTCATCCACACGGCTGGGCTGGTAGAC
  GTGTTTGGCAGGGCCAGTCCCAAGACCATCCATGAGGTCAACGTGCAGGGTACCCGGAAC
  GTGATCGAGGCTTGTGTGCAGACCGGAACACGGTTCCTGGTCTACACCAGCAGCATGGAA
  GTTGTGGGGCCTAACACCAAAGGTCACCCCTTCTACAGGGGCAACGAAGACACCCCATAC
  GAAGCAGTGCACAGGCACCCCTATCCTTGCAGCAAGGCCCTGGCCGAGTGGCTGGTCCTG
  GAGGCCACGGGAGGAAGGTCCGTGGNGGGCTGCCCCTGGTGACGTGTGCCTTTCGTCCCA
  CGGCATCTACGGTGAAGGCCACCAGATCATGAGGNACTNCTACCGCCAGGNCCTGCGCCT
  GGGAGGNTGGCTCTTCGGNCCATCCNGNCTCTGTGGACATGGCCGGTCTAGTGGGCATGT
  GCCTGGAGCACTGCTGCACCCGGAACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025193 unedited
  NGGGGGNNNNNNNNNNNNNTTTTNNNNNNANNNNTAATCTNTGNNACGCGGNCCGCATTT
  NANGATCGAGNNNTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGGGGATGAAGGT
  TTTAAACCGGGGTCAACCAGGTACAACATTGGGGACATCCCCCAGCCCAAGGCTGGCGAT
  GTGCTGGGAAGGAACTTAAGTAAAAAGGGGGGAAGTGAAAACATAAGGAGGCCCTAACCC
  TCCAAAGGGGAATTTTAAAACAAACAGTTGAAGGGGAGGCCTTTGAAAAACAATGGGAAC
  ATCACCTCCCAAAAAGGGACTGAGGGGGCTGGAGGAACCAAAACCGCCTTTGCACTTTGC
  ACCGAGGGTCCCGTGTGGCTGTCAAGAAAACAACGTAAGCCTGTGGAGCCTCTGGTCGCT
  TTCTGCTCAAAAAGCCAAAAGAAAACTGGGGCAGGCCACCAGGGCACCAAACACTTACCA
  AGCGGGCATTANGACTGGGATGAGAGGACAGAGGAAGGACACTCTGTCCCCAAACCCTTC
  CGTGTCCTGACGGGCCTAGAACTTGGAGGTGTCAAGACACAACACAGACTCAAGGCTTCT
  GGATTGAAAATGTGGGAGGCTTGGGCCAAGCGCGGTGGCTCACACTTGTAGTTCCAACGT
  TTTGGGAAGGCCACGTGGGAAGATCACTTGAGCCCAGAGTTCAAGACCAAGCTGAGCATA
  TAGTGAGACCCTGTCCTTAAAACAACAAAAAAAAGCCAGTGTGTTGGCGTGCACCTGTGG
  CCCNACTGTTCACGAAGCTTAGGCAGGAGATATTCTTAAACCACAGAGGTGTAGGGGGCA
  TGACCTATTATTACCCCACTTGCCTCCAGTTTGGGGACCAAGCAGAACCTGTCTCAAAAG
  AAAAAGAAATTGAAGGGCTTTANAAAGGAAGNCATTCTGGCCCAGAGCCTGGCTCTGCTC
  TGAATGCACTGCCCTTCCGCACAGGGTGAGGGCTCGGCCTCGGGGTATGGCTGATCTGGG
  CTCCAGGCAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025193
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025193.2, NP_079469.2
• RefSeq Size: 2189 bp
• RefSeq ORF: 1110 bp
• Locus ID: 80270
• UniProt ID: Q9H2F3
• Cytogenetics: 16p11.2
• Domains: 3Beta_HSD
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis
• Gene Summary: This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (a).