Mesothelin (MSLN) (NM_005823) Human Untagged Clone

CAT#: SC110135

MSLN (untagged)-Human mesothelin (MSLN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Mesothelin
• Synonyms: MPF; SMRP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110135 sequence for NM_005823 edited (data generated by NextGen Sequencing)
  ATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCCCTCGGCAGC
  CTCCTGTTCCTGCTCTTCAGCCTCGGATGGGTGCAGCCCTCGAGGACCCTGGCTGGAGAG
  ACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAACATTTCCAGC
  CTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTGAGCACGGAG
  CGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGCTCTCAACAGAGCAG
  CTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCCCTCCCATTG
  GACCTGCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGCACCCGTTTC
  TTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCCGAGCGACAG
  CGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGGGGTCTCTGCTGAGCGAGGCT
  GATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCTTTGTGGCCGAGTCG
  GCCGAAGTGCTGCTACCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCAGGACCAGCAG
  GAGGCAGCCAGGGCGGCTCTGCAGGGCGGGGGACCCCCCTACGGCCCCCCGTCGACATGG
  TCTGTCTCCACGATGGACGCTCTGCGGGGCCTGCTGCCCGTGCTGGGCCAGCCCATCATC
  CGCAGCATCCCGCAGGGCATCGTGGCCGCGTGGCGGCAACGCTCCTCTCGGGACCCATCC
  TGGCGGCAGCCTGAACGGACCATCCTCCGGCCGCGGTTCCGGCGGGAAGTGGAGAAGACA
  GCCTGTCCTTCAGGCAAGAAGGCCCGCGAGATAGACGAGAGCCTCATCTTCTACAAGAAG
  TGGGAGCTGGAAGCCTGCGTGGATGCGGCCCTGCTGGCCACCCAGATGGACCGCGTGAAC
  GCCATCCCCTTCACCTACGAGCAGCTGGACGTCCTAAAGCATAAACTGGATGAGCTCTAC
  CCACAAGGTTACCCCGAGTCTGTGATCCAGCACCTGGGCTACCTCTTCCTCAAGATGAGC
  CCTGAGGACATTCGCAAGTGGAATGTGACGTCCCTGGAGACCCTGAAGGCTTTGCTTGAA
  GTCAACAAAGGGCACGAAATGAGTCCTCAGGTGGCCACCCTGATCGACCGCTTTGTGAAG
  GGAAGGGGCCAGCTAGACAAAGACACCCTAGACACCCTGACCGCCTTCTACCCTGGGTAC
  CTGTGCTCCCTCAGCCCCGAGGAGCTGAGCTCCGTGCCCCCCAGCAGCATCTGGGCGGTC
  AGGCCCCAGGACCTGGACACGTGTGACCCAAGGCAGCTGGACGTCCTCTATCCCAAGGCC
  CGCCTTGCTTTCCAGAACATGAACGGGTCCGAATACTTCGTGAAGATCCAGTCCTTCCTG
  GGTGGGGCCCCCACGGAGGATTTGAAGGCGCTCAGTCAGCAGAATGTGAGCATGGACTTG
  GCCACGTTCATGAAGCTGCGGACGGATGCGGTGCTGCCGTTGACTGTGGCTGAGGTGCAG
  AAACTTCTGGGACCCCACGTGGAGGGCCTGAAGGCGGAGGAGCGGCACCGCCCGGTGCGG
  GACTGGATCCTACGGCAGCGGCAGGACGACCTGGACACGCTGGGGCTGGGGCTACAGGGC
  GGCATCCCCAACGGCTACCTGGTCCTAGACCTCAGCATGCAAGAGGCCCTCTCGGGGACG
  CCCTGCCTCCTAGGACCTGGACCTGTTCTCACCGTCCTGGCACTGCTCCTAGCCTCCACC
  CTGGCCTGA
  
  Clone variation with respect to NM_005823.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005823 unedited
  CCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
  CTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTC
  GGCACGAGCATGGCCTTGCCAACGGCTCGACCCCTGTTGGGGTCCTGTGGGACCCCCGCC
  CTCGGCAGCCTCCTGTTCCTGCTCTTCAGCCTCGGATGGGTGCAGCCCTCGAGGACCCTG
  GCTGGAGAGACAGGGCAGGAGGCTGCGCCCCTGGACGGAGTCCTGGCCAACCCACCTAAC
  ATTTCCAGCCTCTCCCCTCGCCAACTCCTTGGCTTCCCGTGTGCGGAGGTGTCCGGCCTG
  AGCACGGAGCGTGTCCGGGAGCTGGCTGTGGCCTTGGCACAGAAGAATGTCAAGCTCTCA
  ACAGAGCAGCTGCGCTGTCTGGCTCACCGGCTCTCTGAGCCCCCCGAGGACCTGGACGCC
  CTCCCATTGGACCTGCTGCTATTCCTCAACCCAGATGCGTTCTCGGGGCCCCAGGCCTGC
  ACCCGTTTCTTCTCCCGCATCACGAAGGCCAATGTGGACCTGCTCCCGAGGGGGGCTCCC
  GAGCGACAGCGGCTGCTGCCTGCGGCTCTGGCCTGCTGGGGTGTGCGGNGGTCTCTGCTG
  AGCGAGGCTGATGTGCGGGCTCTGGGAGGCCTGGCTTGCGACCTGCCTGGGCGCTTTGTG
  GCCGAGTNCGCCGAAGTGCTGCTACCCCCGGCTGGTGAGCTGCCCGGGACCCCTGGACCA
  NGACCAGCANNGAGCANCCAGGGCGCTTCNGGGNGGGGGGGGGACCCCCCTACGGCCCCC
  CGTCGACATGGTCTGTCTCCACGATGGACGCTCCTGCGGGGCCTGCTNGCCGTGCTGGGC
  CAGCCCATCACCGCAGCATCCCGCAGGGCATCGTGGCCCGCTGGCGGCAACGCTCCTCTC
  GGGACCATTCTTGGCGCAGCTGAACGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005823 unedited
  GCCTTACGACCCGCGGCCCGCAATCTAGAGTCCGAGCCCCCCCCCCCCCCCCCCCCCCCT
  TCAACGTGTCTGCAGGGGGCATGTTCCCGTTTACTGAGCGCGAGTTCTCTTGGGGTGGAA
  CGGGGACCACCCGTGCCTGCTCCTGGCCAGGCGGGGATCCCCAGCAGGGCTGGGCCCAGC
  AAGGGAGTGGGGCCCTCAGGCCAGGGTGGAGGCTAGGACCAGTGCCAGGACGGTGAAAAC
  AGGTCCAGGTCCTAAGAGGCAGGGCGTCCCCGAGAGGGCCTCTTGCATGCTGAGGTCTAG
  GACCAGGTACCCGTTGGGGATGCCGCCCTGTAGCCCCAGCCCCAGCGTGTCCAGGTCGTC
  CTGCCGCTGCCGTAAGATCCAGTCCCGCACCGGGCGGTGCCGCTCCTCCGCCTTCAGGCC
  CTCCACGTGGGGTCCCAAAAGTTTCTGCACCTCAGCCACAGTCAACGGCAGCACCGCATC
  CGTCCGCAGCTTCATGAACGTGGCCAAGTCCATGCTCACATTCTGCTGACTGAGCGCCTT
  CAAATCCTCCGTGGGGGCCCCACCCAGGAAGGACTGGATCTTCACGAAGTATTCGGACCC
  GTTCATGTTCTGGAAAGCAAGGCGGGCCTTGGGATAAAGGACGTCCAGCTGCCTTGGGTC
  ACACGTGTCCAGGTCCTGGGGCCTGACCGCCCAAATGCTGCTGGGGGGCACGGAACTCAT
  CTCCTCGGGGCCTGAGGAGCACAGGTACCCAGGGTAAAAAGCGGTCAAGGTGTCCAAGGT
  GTCTTTTTCTAACTGCCCCCTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005823
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005823.4, NP_005814.2
• RefSeq Size: 2052 bp
• RefSeq ORF: 1869 bp
• Locus ID: 10232
• UniProt ID: Q13421
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1).