MACROH2A1 (NM_004893) Human Untagged Clone

CAT#: SC110068

H2AFY (untagged)-Human H2A histone family, member Y (H2AFY), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MACROH2A1
• Synonyms: H2A.y; H2A/y; H2AF12M; H2AFY; MACROH2A1.1; macroH2A1.2; mH2A1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004893, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGAGCCGCGGTGGGAAGAAGAAGTCCACCAAGACGTCCAGGTCTGCCAAAGCAGGAGTCATCTTTC
  CCGTGGGGCGGATGCTGCGGTACATCAAGAAAGGCCACCCCAAGTACAGGATTGGAGTGGGGGCACCCGT
  GTACATGGCCGCCGTCCTGGAATACCTGACAGCGGAGATTCTGGAGCTGGCTGGCAATGCAGCGAGAGAC
  AACAAGAAGGGACGGGTCACACCCCGGCACATCCTGCTGGCTGTGGCCAATGATGAAGAGCTGAATCAGC
  TGCTAAAAGGAGTCACCATAGCCAGTGGGGGTGTGTTACCCAACATCCACCCCGAGTTGCTAGCGAAGAA
  GCGGGGATCCAAAGGAAAGTTGGAAGCCATCATCACACCACCCCCAGCCAAAAAGGCCAAGTCTCCATCC
  CAGAAGAAGCCTGTATCTAAAAAAGCAGGAGGCAAGAAAGGGGCCCGGAAATCCAAGAAGCAGGGTGAAG
  TCAGTAAGGCAGCCAGCGCCGACAGCACAACCGAGGGCACACCTGCCGACGGCTTCACAGTCCTCTCCAC
  CAAGAGCCTCTTCCTTGGCCAGAAGCTGAACCTTATTCACAGTGAAATCAGTAATTTAGCCGGCTTTGAG
  GTGGAGGCCATAATCAATCCTACCAATGCTGACATTGACCTTAAAGATGACCTAGGAAACACGCTGGAGA
  AGAAAGGTGGCAAGGAGTTTGTGGAAGCTGTCCTGGAACTCCGGAAAAAGAACGGGCCCTTGGAAGTAGC
  TGGAGCTGCTGTCAGCGCAGGCCATGGCCTGCCTGCCAAGTTTGTGATCCACTGTAATAGTCCAGTTTGG
  GGTGCAGACAAGTGTGAAGAACTTCTGGAAAAGACAGTGAAAAACTGCTTGGCCCTGGCTGATGATAAGA
  AGCTGAAATCCATTGCATTTCCATCCATCGGCAGCGGCAGGAACGGTTTTCCAAAGCAGACAGCAGCTCA
  GCTGATTCTGAAGGCCATCTCCAGTTACTTCGTGTCTACAATGTCCTCTTCCATCAAAACGGTGTACTTC
  GTGCTTTTTGACAGCGAGAGTATAGGCATCTATGTGCAGGAAATGGCCAAGCTGGACGCCAACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004893 unedited
  CATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGACTCGAGGGTCAAGGCCGG
  GGCGCCGGGCGGCACGGGCGCGGAGGCCGGACGCTCGGGGCCGCGAAGGGATGTGACGAG
  CGGCGCGCTGTGCATTGTGGGAAGCCGGCCGCGAGGACTGGTTCCAGTTCACTCGGCAGC
  GGCGCCGGGCGGAGGGGGAGAGCGCGGGCCGCGCGGGCGGGAAGCGAAGAGGCGGGCGGG
  CCAGCGAGGAGCGCGGAGAGAAAAGGCGCGAGCGGCCAGGAGGGCTCAGGCCGAGACACC
  TTGCAGCTGCCGCCGCCGCCACCGAGCCGCCGCTGTGCTCACTGATCCGCCTCCAGGGCC
  ACCGCCATGTCGAGCCGCGGTGGGAAGAAGAAGTCCACCAAGACGTCCAGGTCTGCCAAA
  GCAGGAGTCATCTTTCCCGTGGGGCGGATGCTGCGGTACATCAAGAAAGGCCACCCCAAG
  TACAGGATTGGAGTGGGGGCACCCGTGTACATGGCCGCCGTCCTGGAGTACCTGACAGCG
  GAGATTCTGGAGCTGGCTGGCAATGCAGCGAGAGACAACAAGAAGGGACGGGTCACACCC
  CGGCACATCCTGCTGGCTGTGGCCAATGATGAAGAGCTGAATCAGCTGCTAAAAGGAGTC
  ACCATAGCCAGTGGGGGTGTGTTACCCCACATCCACCCCGAGTTGCTAGCGAACAAGCGG
  GGATCCAAAGGAAAGTTGGAAGCCATCATCACACCACCCCCCAGCCCAAAAGGCCAAGTT
  CTCCAATCCCAGAGTAAGCCTGTTTCCTAAAAAGCATGAAGCACAGAAGGGTGCCCCGGA
  AATCCAAGAAACCAGGTTGAGTCCATTAAGGCAGCCAGTGCCGACAGCACAACCGAGGGC
  CACCTGCCGAAGGCTTCCAGTTCTTTTCCCCAATAGCCTCTTCTTGGCCACAATTGCAGT
  TGTCAAGCTGACTTTGCTCCATCGCACGGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004893 unedited
  NGCCTTACTTGNCCGCGCCCGCTATCTANGATCGGTTTTTCTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACGGGTAAACATTTTACTA
  AATCAACTCACACAAATTCCAAATTGCAAATTTAATTAAGGACAACAGATTCTGGTTTGC
  CTTATTAAATCTTTCATTTTCAAACCCCTCAATAAAACAAAAAGCACCCCAAAATTAGGT
  CTGGGGCAAATTCATGATTCTTTTGGGGGGGGAACCCAAAAAAACCTTAAAGTAAAAAAA
  ACCCCCCTGGGGGATGTCCAATAAAGCCCCACAACACCCGCTTACAAACGGGGCTCCCTG
  GCTTCGGGGACAGGAAAAAAACCCTGTCCTCCCCACTGGTGGGCGGCAATCAGGGTTTCC
  TTAAAACAAAACTATAAAACCCCCTAGGTTACACTAAGTCAAACCCCGTCTGGAACACAG
  GGTTTAACAACAGAAATGCCCACTATAAGGGTCAACATAAAAACTTTTTAAAAGGGCAAA
  AACAATTAAACCGGAACCCCAAGCCTTATTTTCCCTAAATGACCAAACCTGAAAAAGAAA
  GGGGCCCCCCCTCCCACTAGGAGGGAAGGGGCTTTTTTTTTTTTTTTAAACTGAAGGGGG
  GGCCACTGGGGCACCCGTTTTTGTCCTTTCTCACACTAATTGGGCCCCACCTTGGCCCTT
  TCCTGCCAAAAAACGCCTATACTCCCTGTGCCAAAAGCACCAAACCCCCCCTTTGGTGGA
  AAAGCCCTTGGAACCCCAAAAACTGGAAGGCCCCTCAAAAACCCTGACTTTCCGTCGTCT
  TGAAAACCCCTTCCCCCCCCCCCCAGGGGGGAACACCACGGACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004893
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004893.2, NP_004884.1
• RefSeq Size: 1923 bp
• RefSeq ORF: 1116 bp
• Locus ID: 9555
• UniProt ID: O75367
• Cytogenetics: 5q31.1
• Domains: H2A, A1pp, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (2) has a unique 5' UTR and uses an alternate splice site in its coding region, compared to variant 3. This results in a protein (isoform 2) that is a single amino acid shorter than isoform 3. Variants 2 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.