OGT (NM_181673) Human Untagged Clone

SKU SC109934

OGT (untagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: OGT
• Synonyms: HINCUT-1; HRNT1; MRX106; O-GLCNAC; OGT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109934 sequence for NM_181673 edited (data generated by NextGen Sequencing); ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGCACAGGGTTAGCTGAGTTGGCACATCGA; GAATATCAGGCAGGAGATTTTGAGGCAGCTGAGAGACACTGCATGCAGCTCTGGAGACAA; GAGCCAGACAATACTGGTGTGCTTTTATTACTTTCATCTATACACTTCCAGTGTCGAAGG; CTGGACAGATCTGCTCACTTTAGCACTCTGGCAATTAAACAGAACCCCCTTCTGGCAGAA; GCTTATTCGAATTTGGGGAATGTGTACAAGGAAAGAGGGCAGTTGCAGGAGGCAATTGAG; CATTATCGACATGCATTGCGTCTCAAACCTGATTTCATCGATGGTTATATTAACCTGGCA; GCCGCCTTGGTAGCAGCGGGTGACATGGAAGGGGCAGTACAAGCTTACGTCTCTGCTCTT; CAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGGGGAACCTGCTCAAAGCCCTG; GGTCGCTTGGAAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCAACCGAACTTT; GCAGTAGCTTGGAGTAATCTTGGCTGTGTTTTCAATGCACAAGGGGAAATTTGGCTTGCA; ATTCATCACTTTGAAAAGGCTGTCACCCTTGACCCAAACTTTCTGGATGCTTATATCAAT; TTAGGAAATGTCTTGAAAGAGGCACGCATTTTTGACAGAGCTGTGGCAGCTTATCTTCGT; GCCCTAAGTTTGAGTCCAAATCACGCAGTGGTGCACGGCAACCTGGCTTGTGTATACTAT; GAGCAAGGCCTGATAGATCTGGCAATAGACACCTACAGGCGGGCTATCGAACTACAACCA; CATTTCCCTGATGCTTACTGCAACCTAGCCAATGCTCTCAAAGAGAAGGGCAGTGTTGCT; GAAGCAGAAGATTGTTATAATACAGCTCTCCGTCTGTGTCCCACCCATGCAGACTCTCTG; AATAACCTAGCCAATATCAAACGAGAACAGGGAAACATTGAAGAGGCAGTTCGCTTGTAT; CGTAAAGCATTAGAAGTCTTCCCAGAGTTTGCTGCTGCCCATTCAAATTTAGCAAGTGTA; CTGCAGCAGCAGGGAAAACTGCAGGAAGCTCTGATGCATTATAAGGAGGCTATTCGAATC; AGTCCTACCTTTGCTGATGCCTACTCTAATATGGGAAACACTCTAAAGGAGATGCAGGAT; GTTCAGGGAGCCTTGCAGTGTTATACGCGTGCCATCCAAATTAATCCTGCATTTGCAGAT; GCACATAGCAATCTGGCTTCCATTCATAAGGATTCAGGGAATATTCCAGAAGCCATAGCT; TCTTACCGCACGGCTCTGAAACTTAAGCCTGATTTTCCTGATGCTTATTGTAACTTGGCT; CATTGCCTGCAGATTGTCTGTGATTGGACAGACTATGATGAGCGAATGAAGAAGTTGGTC; AGTATTGTGGCTGACCAGTTAGAGAAGAATAGGTTGCCTTCTGTGCATCCTCATCATAGT; ATGCTATATCCTCTTTCTCATGGCTTCAGGAAGGCTATTGCTGAGAGGCACGGCAACCTG; TGCTTAGATAAGATTAATGTTCTTCATAAACCACCATATGAACATCCAAAAGACTTGAAG; CTCAGTGATGGTCGGCTGCGTGTAGGATATGTGAGTTCCGACTTTGGGAATCATCCTACT; TCTCACCTTATGCAGTCTATTCCAGGCATGCACAATCCTGATAAATTTGAGGTGTTCTGT; TATGCCCTGAGCCCAGACGATGGCACAAACTTCCGAGTGAAGGTGATGGCAGAAGCCAAT; CATTTCATTGATCTTTCTCAGATTCCATGCAATGGAAAAGCAGCTGATCGCATCCATCAG; GATGGAATTCATATCCTTGTAAATATGAATGGCTATACTAAGGGCGCTCGAAATGAGCTT; TTTGCTCTCAGGCCAGCTCCTATTCAGGCAATGTGGCTGGGATACCCTGGGACGAGTGGT; GCGCTTTTCATGGATTATATTATCACTGATCAGGAAACTTCGCCAGCTGAAGTTGCTGAG; CAGTATTCCGAGAAATTGGCTTATATGCCCCACACTTTTTTTATTGGTGATCATGCTAAT; ATGTTCCCTCACCTGAAGAAAAAAGCAGTCATCGATTTTAAGTCCAATGGGCACATTTAT; GACAATCGGATAGTTCTGAATGGCATCGACCTCAAAGCATTTCTTGATAGTCTACCAGAT; GTGAAAATTGTCAAGATGAAGTGTCCTGATGGAGGAGACAATGCAGATAGCAGTAACACA; GCTCTTAATATGCCTGTTATTCCTATGAATACTATTGCAGAAGCAGTTATTGAAATGATT; AACCGAGGACAGATTCAAATAACAATTAATGGATTCAGTATTAGCAATGGACTGGCAACT; ACTCAGATCAACAATAAGGCTGCAACTGGAGAGGAGGTTCCCCGTACCATTATTGTAACC; ACCCGTTCTCAGTACGGGTTACCAGAAGATGCCATCGTATACTGTAACTTTAATCAGTTG; TATAAAATTGACCCTTCTACTTTGCAGATGTGGGCAAACATTCTGAAGCGTGTTCCCAAT; AGTGTACTCTGGCTGTTGCGTTTTCCAGCAGTAGGAGAACCTAATATTCAACAGTATGCA; CAAAACATGGGCCTGCCCCAGAACCGTATCATTTTTTCACCTGTTGCTCCTAAAGAGGAA; CACGTCAGGAGAGGCCAGCTGGCTGATGTCTGCTTGGACACTCCACTCTGTAATGGGCAC; ACCACAGGGATGGATGTCCTCTGGGCAGGGACCCCCATGGTGACTATGCCAGGAGAGACT; CTTGCTTCTCGAGTTGCAGCATCCCAGCTCACTTGCTTAGGTTGTCTTGAGCTTATTGCT; AAAAACAGACAAGAATATGAAGACATAGCTGTGAAGCTGGGAACTGATCTAGAATACCTG; AAGAAAGTTCGTGGCAAAGTCTGGAAGCAAAGAATATCTAGCCCTCTGTTCAACACCAAA; CAATACACAATGGAACTAGAGCGGCTCTATCTACAGATGTGGGAGCATTATGCAGCTGGC; AACAAACCTGACCACATGATTAAGCCTGTTGAAGTCACTGAGTCAGCATAA; ; Clone variation with respect to NM_181673.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181673 unedited; CCGCGNNCCCGGNNCNCNCCCCCNCCTTTTNNNNNNCNCCCCGTTCAGAAATTGTNATAC; CACTCACTNATAGGCGGCCGCGNAATTCGCACGAGNAGAGGTCAATTAGAGTTCCCAGGG; TTTGAAGCCTGTAACTGCTGCCGCCGCTCAAGCCCTCCAGAGCATTGCTACGGCTGCTGC; CCTTGTACTACTACCTCCAAATACGTTCTTGCTGGTAGTGGCGGCAGCAGGACCAATTAC; CTCTTTTTGCTCTCCCTCGAGAAGCTCCAGATGGCGTCTTCCGTGGGCAACGTGGCCGAC; AGCACAGGGTTAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCT; GAGAGACACTGCATGCAGCTCTGGAGACAAGAGCCAGACAATACTGGTGTGCTTTTATTA; CTTTCATCTATACACTTCCAGTGTCGAAGGCTGGACAGATCTGCTCACTTTAGCACTCTG; GCAATTAAACAGAACCCCCTTCTGGCAGAAGCTTATTCGAATTTGGGGAATGTGTACAAG; GAAAGAGGGCAGTTGCAGGAGGCAATTGAGCATTATCGACATGCATTGCGTCTCAAACCT; GATTTCATCGATGGTTATATTAACCTGGCAGCCGCCTTGGTAGCAGCGGGTGACATGGAA; GGGGCAGTACAAGCTTACGTCTCTGCTCTTCAGTACAATCCTGATTTGTACTGTGTTCGC; AGTGACCTGNGGAACCTGCTCAAAGCCCTGNGTCGCTTGGAAGAGCCCAAGCATGTTATT; TGAAAGCAATTGAGACGCACCCGAACTTTGCAGTAGCCTTGGAGTATCTGGGCTGTGTTN; TTAATGCACAAGGGGAAATTTGCCTTGCAATTCATCACTTTGNAAAGGCCTGTCACCCTT; GACCCAAACTTTTCTGGAGCTTATATCAATTTAGAAAAGTCTTGAAAGAGCACGCATTTT; TGACAGAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_181673
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181673.1, NP_858059.1
• RefSeq Size: 5445 bp
• RefSeq ORF: 3111 bp
• Locus ID: 8473
• UniProt ID: O15294
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Summary: This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.