PPP2R4 (PTPA) (NM_021131) Human Untagged Clone

CAT#: SC109606

PPP2R4 (untagged)-Human protein phosphatase 2A activator, regulatory subunit 4 (PPP2R4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R4
• Synonyms: PP2A; PPP2R4; PR53
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109606 sequence for NM_021131 edited (data generated by NextGen Sequencing)
  ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
  CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
  CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
  GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAGTCGCTCTT
  CTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTT
  GGGAATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTG
  GCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAG
  GAGTCAGTGGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCT
  GCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATT
  GTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGG
  ATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTC
  ATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGAT
  GAGAAGGCCGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATT
  ACCGAGATGAAGACTGGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCC
  GTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTG
  GAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTC
  ACGTTGGGCTAG
  
  Clone variation with respect to NM_021131.4
  965 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021131 unedited
  GGGTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCTGGCC
  GGAGCAAGATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTC
  CAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCA
  AATGGAAGCGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAG
  GTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAG
  TCGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCT
  CTCGGTTTGGGAATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAA
  ACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTT
  ACCTAAAGGAGTCAGTGGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAG
  CCTTCGCTGCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAA
  TAGCTATTGTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAA
  CATACAGGATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTC
  TGCCCTTCATCTGGNGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACT
  TTGTGGATGAGAAGGCCGTNGATGAGAACCACANGACTACATNGTCCTGGAGTGTATCCT
  GTTTATACCGAGAGAAGACTGGCCCATTTGCAGACACTCCACCAGCTGGGAACATCAGCG
  CGTCCTTTCTGNTCCAAGTGAACAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021131 unedited
  AGCTTGGCCGCGGCCGCATCTANAGTCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTACAG
  GGGCAAAACAGAGGCTTTAATCTGAACAGCAATGAGGGTGCAAGGGGCTGGGAGCCCATA
  GGGGGATGGGGGAGCCAGGAAGGGGTGTCTGGCCCTGGGAGAGAGGTGGACGCTGGGCCT
  CTTCTGAGAAACTGTCACTGACCTTTGGGACTCCTGACCCAGCCTCTGCCCAGACCATCA
  CCGGATCCCAGGCTGAGCCCCTGTGCTGTGCTTCTCAGTGCTCCCCCTTCCTGAAGGGTG
  TGTGGACCCAGACACAGGAGGGGGCAGTGGAGAAGGGCAGGCCTGGGGGACGAGCCTGTA
  AGAGCCAGGCCCAGGAACAGCCCTAGGAGAAACCTCGGCGGTGGCCCTCAATGGGCCTGC
  CCAGCCCCCACTGGGCCCATTCTGAGTGCCAAAGCCTGGGCTTGGGAGGCTGTCTCTTAC
  CTCACCCTATAAAAGCAATGGACAGTTCAGGGAGGTGGGAGGACAAGGTGTGGGACTGCC
  ACCTTCTGGGCCCACCCATCACCCAGGGGAGCAGAGAAGCAGGGCCCAGGGGCCCAGGGT
  GAGGCTCACAGCAGCGCAAAGGTAGCAGAAGTGGCCGGGCCTAAGACTGCCAAGAGGAAT
  CCAGCTCTCCAATACAATGGGCCCCAAGCCCCCTCCTGATTTTTGCCCTCCCTCCCTGCC
  AGGAAAGTCTGGAAGACTGCCTCACCCATATCTGGCCCTCCAGAGCCCGCCCTACCCAAG
  GCGCTTCATTGCTAGTACCCTAAGGACGGAGAATGGGGTACGCAGAAACCCAAGCCAGGC
  TTGAACTGANACCCGGGGAGGTTGCACCGGGCCTAGCCCGGGCAGTGAAAGACCTGAGGG
  CCACCCACTTAACGCCTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021131
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021131.3, NP_066954.2
• RefSeq Size: 2666 bp
• RefSeq ORF: 972 bp
• Locus ID: 5524
• UniProt ID: Q15257
• Cytogenetics: 9q34.11
• Domains: PTPA
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame segment of the coding region, compared to variant 1. Variants 2 and 3 encode a shorter protein (isoform b), also known as isoform alpha, that is missing an internal segment compared to isoform a.