PMP22 (NM_000304) Human Untagged Clone

CAT#: SC109589

PMP22 (untagged)-Human peripheral myelin protein 22 (PMP22), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMP22
• Synonyms: CIDP; CMT1A; CMT1E; DSS; GAS-3; GAS3; HMSNIA; HNPP; Sp110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000304 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCGTCGCTCGGCCCAGTGCGTT
  CGGCCTCACGCCCAGCGCTCTCCTCGCAGGCAGAAACTCCGCTGAGCAGAACTTGCCGCC
  AGAATGCTCCTCCTGTTGCTGAGTATCATCGTCCTCCACGTCGCGGTGCTGGTGCTGCTG
  TTCGTCTCCACGATCGTCAGCCAATGGATCGTGGGCAATGGACACGCAACTGATCTCTGG
  CAGAACTGTAGCACCTCTTCCTCAGGAAATGTCCACCACTGTTTCTCATCATCACCAAAC
  GAATGGCTGCAGTCTGTCCAGGCCACCATGATCCTGTCGATCATCTTCAGCATTCTGTCT
  CTGTTCCTGTTCTTCTGCCAACTCTTCACCCTCACCAAGGGGGGCAGGTTTTACATCACT
  GGAATCTTCCAAATTCTTGCTGGTCTGTGCGTGATGAGTGCTGCGGCCATCTACACGGTG
  AGGCACCCGGAGTGGCATCTCAACTCGGATTACTCCTACGGTTTCGCCTACATCCTGGCC
  TGGGTGGCCTTCCCCCTGGCCCTTCTCAGCGGTGTCATCTATGTGATCTTGCGGAAACGC
  GAATGAGGCGCCCAGACGGTCTGTCTGAGGCTCTGAGCGTACATAGGGAAGGGAGGAAGG
  GAAACCAGAAAGCAGACAAAGAAAAAAGAGCTAGCCCAAAATCCCAAACTCAAACCAAAC
  CAAACAGAAAGCAGTGGAGGTGGGGGTTGCTGTTGATTGAAGATGTATATAATATCTCCG
  GTTTATAAAACCTATTTATAACACTTTTTACATATATGTACATAGTATTGTTTGCTTTTT
  ATGTTGACCATCAGCCTCGTGTTGAGCCTTAAAGAAGTAGCTAAGGAACTTTACATCCTA
  ACAGTATAATCCAGCTCAGTATTTTTGTTTTGTTTTTTGTTTGTTTGTTTTGTTTTACCC
  AGAAATAAGATAACTCCATCTCGCCCCTTCCCTTTCATCTGAAAGAAGATACCTCCCTCC
  CAGTCCACCTCATTTAGAAAACCAAAGTGTGGGTAGAAACCCCAAATGTCCAAAAGCCCT
  TTTCTGGTGGGTGACCCAGTGCATCCAACAGAAACAGCCGCTGCCCGAACCTGTGTGAAG
  CTTTACGCGCACACGGACAAAATGCCCAAACTGGAGCCCTTGCAAAAACACGGCTTGTGG
  CATTGGCATACTTGCCCTTACAGGTGGAGTATCTTCGTCACACATCTAAATGAGAAATCA
  GTGACAACAAGTCTTTGAAATGGTGCTATGGATTTACCATTCCTTATTATCACTAATCAT
  CTAAACAACTCACTGGAAATCCAATTAACAATTTTACAACATAAGATAGAATGGAGACCT
  GAATAATTCTGTGTAATATAAATGGTTTATAACTGCTTTTGTACCTAGCTAGGCTGCTAT
  TATTACTATAATGAGTAAATCATAAAGCCTTCATCACTCCCACATTTTTCTTACGGTCGG
  AGCATCAGAACAAGCGTCTAGACTCCTTGGGACCGTGAGTTCCTAGAGCTTGGCTGGGTC
  TAGGCTGTTCTGTGCCTCCAAGGACTGTCTGGCAATGACTTGTATTGGCCACCAACTGTA
  GATGTATATATGGTGCCCTTCTGATGCTAAGACTCCAGACCTTTTGTTTTTGCTTTGCAT
  TTTCTGATTTTATACCAACTGTGTGGACTAAGATGCATTAAAATAAACATCAGAGTAACT
  CAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000304 unedited
  GGCACATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGCGTCGCTCGGCCCAGTGCGTTCGGCCTCACGCCCAGCGCTCTCCTC
  GCAGGCAGAAACTCCGCTGAGCAGAACTTGCCGCCAGAATGCTCCTCCTGTTGCTGAGTA
  TCATCGTCCTCCACGTCGCGGTGCTGGTGCTGCTGTTCGTCTCCACGATCGTCAGCCAAT
  GGATCGTGGGCAATGGACACGCAACTGATCTCTGGCAGAACTGTAGCACCTCTTCCTCAG
  GAAATGTCCACCACTGTTTCTCATCATCACCAAACGAATGGCTGCAGTCTGTCCAGGCCA
  CCATGATCCTGTCGATCATCTTCAGCATTCTGTCTCTGTTCCTGTTCTTCTGCCAACTCT
  TCACCCTCACCAAGGGGGGCAGGTTTTACATCACTGGAATCTTCCAAATTCTTGCTGGTC
  TGTGCGTGATGAGTGCTGCGGCCATCTACACGGTGAGGCACCCGGAGTGGCATCTCAACT
  CGGATTACTCCTACGGTTTCGCCTACATCCTGGCCTGGGTGGCCTTCCCCCTGGCCCTTC
  TCAGCGGCGTCATCTATGTGATCTTGCGGAAACGCGAATGAGGCGCCCAGACGGTCTGTC
  TGAGGCTCTGAGCGTCATAGGGGAAGGAGGAAGGGAAACCATAAAGCAGACATAGAACAA
  AGAGCTAGCCCAAAATCCCAAACTCANCCCAAACCCNACAGAAAGCCCCGGAGGCGGGGN
  TTGCTGTTGATTGAAGAGTATATAATATCTCCGGTNTATATAACCTAATTATAACACTTT
  TTACATATATGGACCATAGATTGNNNGGCTTTTTATGTTGACCCATCANCCTCGTGTTGA
  GCCCTAAAGCACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000304 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGTTACTCTGATGTTTATTT
  TAATGCATCTTAGTCCACACAGTTGGTATAAAATCAGAAAATGCAAAGCAAAAACAAAAG
  GTCTGGAGTCTTAGCATCAGAAGGGCACCATATATACATCTACAGTTGGTGGCCAATACA
  AGTCATTGCCAGACAGTCCTTGGAGGCACAGAACAGCCTAGACCCAGCCAAGCTCTAGGA
  ACTCACGGTCCCAAGGAGTCTAGACGCTTGTTCTGATGCTCCGACCGTAAGAAAAATGTG
  GGAGTGATGAAGGCTTTATGATTTACTCATTATAGTAATAATAGCAGCCTAGCTAGGTAC
  AAAAGCAGTTATAAACCATTTATATTACACAGAATTATTCAGGTCTCCATTCTATCTTAT
  GTTGTAAAATTGTTAATTGGATTTCCAGTGAGTTGTTTAGATGATTAGTGATAATAAGGA
  ATGGTAAATCCATAGCACCATTTCAAAGACTTGTTGTCACTGATTTCTCATTTAGATGTG
  TGACGAAGATACTCCACCTGTAAGGGCAAGTATGCCAATGCCACAAGCCGTGTTTTTGCA
  AGGGCTCCAGTTTGGGCATTTTGTCCGTGTGCGCGTAAAGCTTCACACAGGTTCGGGCAG
  CGGCTGTTTCTGTTGGATGCACTGGGTCACCCACCAGAAAGGGGCTTTTGACATTTGGGG
  TTTCTACCCCACTTTGGGTTTCTAATGAGGTGGACTGGGAGGGAAGTATCTTCTTTCAAA
  TGAAAGGGAAGGGGCGAGATGGAGTTATCTTAATTNCTGGGTANACCAAACCANACANCC
  AAAAACAAACCAAAATCCTGAGCTGGNATATACTGTTAGGATGTTAAAGTTCCTTACCTA
  CTTCTTAAGGCTCAACACGAGGCTGAAGGTCACATAAAAGCCAACAATCTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000304
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000304.2, NP_000295.1
• RefSeq Size: 1828 bp
• RefSeq ORF: 483 bp
• Locus ID: 5376
• UniProt ID: Q01453
• Cytogenetics: 17p12
• Protein Families: Transmembrane
• Gene Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1-5 encode the same isoform (1).