hnRNP U (HNRNPU) (NM_004501) Human Untagged Clone

CAT#: SC109317

HNRNPU (untagged)-Human heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hnRNP U
• Synonyms: DEE54; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1; HNRPU; pp120; SAF-A; SAFA; U21.1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004501, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCCTCGCCTGTTAATGTAAAAAAGCTGAAGGTGTCGGAGCTGAAAGAGGAGCTCAAGAAGCGAC
  GCCTTTCTGACAAGGGTCTCAAGGCCGAGCTCATGGAGCGACTCCAGGCTGCGCTGGACGACGAGGAGGC
  CGGGGGCCGCCCCGCCATGGAGCCCGGGAACGGCAGCCTAGACCTGGGCGGGGATTCCGCTGGGCGCTCG
  GGAGCAGGCCTCGAGCAGGAGGCCGCGGCCGGCGGCGATGAAGAGGAGGAGGAAGAGGAAGAGGAGGAGG
  AAGGAATCTCCGCTCTGGACGGCGACCAGATGGAGCTAGGAGAGGAGAACGGGGCCGCGGGGGCGGCCGA
  CTCGGGCCCGATGGAGGAGGAGGAGGCCGCCTCGGAAGACGAGAACGGCGACGATCAGGGTTTCCAGGAA
  GGGGAAGATGAGCTCGGGGACGAAGAGGAAGGCGCGGGCGACGAGAACGGGCACGGGGAGCAGCAGCCTC
  AACCGCCGGCGACGCAGCAGCAACAGCCCCAACAGCAGCGCGGGGCCGCCAAGGAGGCCGCGGGGAAGAG
  CAGCGGCCCCACCTCGCTGTTCGCGGTGACGGTGGCGCCGCCCGGGGCGAGGCAGGGCCAGCAGCAGGCG
  GGAGGGGACGGCAAAACAGAACAGAAAGGCGGAGATAAAAAGAGGGGTGTTAAAAGACCACGAGAAGATC
  ATGGCCGTGGATATTTTGAGTACATTGAAGAGAACAAGTATAGCAGAGCCAAATCTCCTCAGCCACCTGT
  TGAAGAAGAAGATGAACACTTCGATGACACAGTGGTTTGTCTTGATACTTATAATTGTGATCTACATTTT
  AAAATATCAAGAGATCGTCTCAGTGCTTCTTCCCTTACAATGGAGAGTTTTGCTTTTCTTTGGGCTGGAG
  GAAGAGCATCCTATGGTGTGTCAAAAGGCAAAGTGTGTTTTGAGATGAAGGTTACAGAGAAGATCCCAGT
  AAGGCATTTATATACAAAAGATATTGACATACATGAAGTTCGTATTGGCTGGTCACTAACTACAAGTGGA
  ATGTTACTTGGTGAAGAAGAATTTTCTTATGGGTATTCTCTAAAAGGAATAAAAACATGCAACTGTGAGA
  CTGAAGATTATGGAGAAAAGTTTGATGAAAATGATGTGATTACATGTTTTGCTAACTTTGAAAGTGATGA
  AGTAGAACTCTCGTATGCTAAGAATGGACAAGATCTTGGCGTTGCCTTCAAAATCAGTAAGGAAGTTCTT
  GCTGGACGGCCACTGTTCCCGCATGTTCTCTGCCACAACTGTGCAGTTGAATTTAATTTTGGTCAGAAGG
  AAAAGCCATATTTTCCAATACCTGAAGAGTATACTTTCATCCAGAACGTCCCCTTAGAGGATCGAGTTAG
  AGGACCAAAGGGGCCTGAAGAGAAGAAAGATTGTGAAGTTGTGATGATGATTGGCTTGCCAGGAGCTGGA
  AAAACTACCTGGGTTACTAAACATGCAGCAGAAAATCCAGGGAAATATAACATTCTTGGCACAAATACTA
  TTATGGATAAGATGATGGTGGCAGGTTTTAAGAAGCAAATGGCAGATACTGGAAAACTGAACACACTGTT
  GCAGAGAGCCCCCCAGTGTCTTGGGAAATTTATTGAGATTGCTGCCCGAAAGAAGCGAAATTTTATTCTG
  GATCAGACAAATGTGTCTGCTGCTGCCCAGAGGAGAAAAATGTGCCTGTTTGCAGGCTTCCAGCGAAAAG
  CTGTTGTAGTTTGCCCAAAAGATGAAGACTATAAGCAAAGAACACAGAAGAAAGCAGAAGTAGAGGGGAA
  AGACCTACCAGAACATGCGGTCCTCAAAATGAAAGGAAACTTTACCCTCCCAGAGGTAGCTGAGTGCTTT
  GATGAAATAACCTATGTTGAACTTCAGAAGGAAGAAGCCCAAAAACTCTTGGAGCAATATAAGGAAGAAA
  GCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACACTGGCTCAAAGAAAAGCAATAAAAATAAGAGTGG
  CAAGAACCAGTTTAACAGAGGTGGTGGCCATAGAGGACGTGGAGGATTCAATATGCGTGGTGGAAATTTC
  AGAGGAGGAGCCCCTGGGAATCGTGGCGGATATAATAGGAGGGGCAACATGCCACAGAGAGGTGGTGGCG
  GTGGAGGAAGTGGTGGAATCGGCTATCCATACCCTCGTGCCCCTGTTTTTCCTGGCCGTGGTAGTTACTC
  AAACAGAGGGAACTACAACAGAGGTGGAATGCCCAACAGAGGGAACTACAACCAGAACTTCAGAGGACGA
  GGAAACAATCGTGGCTACAAAAATCAATCTCAGGGCTACAACCAGTGGCAGCAGGGTCAATTCTGGGGTC
  AGAAGCCATGGAGTCAGCATTATCACCAAGGATATTATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004501 unedited
  GTCGAATTTTGTAATACGAACTCCTATAGGGCGGCCGGCGAATTCGCACGAGGGGCCCTC
  ACCATGAGTTCCTCGCCTGTTAATGTAAAAAAGCTGAAGGTGTCGGAGCTGAAAGAGGAG
  CTCAAGAAGCGACGCCTTTCTGACAAGGGTCTCAAGGCCGAGCTCATGGAGCGACTCCAG
  GCTGCGCTGGACGACGAGGAGGCCGGGGGCCGCCCCGCCATGGAGCCCGGGAACGGCAGC
  CTAGACCTGGGCGGGGATTCCGCTGGGCGCTCGGGAGCAGGCCTCGAGCAGGAGGCCGCG
  GCCGGCGGCGATGAAGAGGAGGAGGAAGAGGAAGAGGAGGAGGAAGGAATCTCCGCTCTG
  GACGGCGACCAGATGGAGCTAGGAGAGGAGAACGGGGCCGCGGGGGCGGCCGACTCGGGC
  CCGATGGAGGAGGAGGAGGCCGCCTCGGAAGACGAGAACGGCGACGATCAGGGTTTCCAG
  GAAGGGGAAGATGAGCTCGGGGACGAAGAGGAAGGCGCGGGCGACGAGAACGGGCACGGN
  GAGCAGCAGCCTCAACCGCCGGCGACGCAGCAGCAACAGCCCCAACAGCAGCGCGGGGCC
  GCCAAGGAGGCCGCGGNGAAGAGCAGCGGCCCCACCTCGCTGTTCGCGGTGACGGTGGCG
  CCGCCCGGNGCGAGGCAGGGCCAGCAGCAGGCGGGAGGGGACGGCAAAACAGAACAGAAA
  GGCGGAGATAAAAGAAGGGTGTTAAAAGACCACGAGAAGATCATGGCCCGTGGATATTTT
  TGAGTACATTGAAGAGGACCAGTATAGCAGAGCCAAATCTCCTCAGCCCCTGTTGAAGAA
  GAAGATGACACTTCGAGNCCCAANGGGTTTGGTCTTGAACTTATAAATTGGGACCTACAT
  TTTAAATTTCAGGAATCCGCCCTCAGTGCTCCTTCCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004501 unedited
  GCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTAACAACAAGTTTTTTTAAAAAAAAAGGGGCAAACCCCCTTT
  TTTTTTCAAAATCAAAAGGCAAAACAAAGGGAAAAATTTGGGGTTTAACCTTCACAAGTT
  GGACCCCCCACAAATAAGGCACCCAAGTTTTACTTTTTAAACAGCCCTTTTACAAACCCC
  CCATTTTTTTTATTTAAGTCCCAAGTTTTAGTTTTCAACCCCAATTATCCCAATTCCATG
  GTTATTTAAAAAAAAAACCTTCCCAGTTTTGGGCAAAAACTATGATTTACCTTACCCCCC
  CCCCTACCCAGGAAACTACAGAGGGGGGGGGGGGGAATTTGACCCGTCCAAAGTTTTAAG
  GCAATTCAGGGGGCCCACTTACCCCTTACAGGAATCGGGGGGCCAACATTTTTATTAAAT
  GGGGACAAGGACCCCCGACTGTTATTCTTTGGGAAAAAGGGGGGGAGTAAATTCTTAATA
  AAAAACCCCCGGGCCAAACCAACATTTTACTTTTTGGGGGAAAAAAAAAAAAAAAGGCCC
  CTTTTACCAAAAAAAAGGGACAACCCCGGAAAACCGGGCACATTCTTTTATCGCGCCCAG
  GGGGGGGGTTTCTTCACAATCCCCTTTTAAACGGGGCTAAAAATGGGCCCACAAAGAAAA
  AAAAAAAATCCCCCCCCCCAGCCTCTAAAAGGGGACCCCGGGCGCTTCCCTTTGGGGGAA
  GGGTAAAAGGTGCCCCCCTAAAAAAAACCGCCCCCCTTCTGGAGGGGTTGGGAAAAAATG
  TTCCAACCCCTTTATTGGGGACCACAAACCCCGGGGGAAAGGGGACCCCCAGGTCTTCGC
  ACCCAAATACGCCCCGCGCCCCGGGGTGGCACCCCGAAAATTATTTCTGCACACCCATCG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004501
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004501.3, NP_004492.2
• RefSeq Size: 6789 bp
• RefSeq ORF: 2421 bp
• Locus ID: 3192
• UniProt ID: Q00839
• Cytogenetics: 1q44
• Domains: SAP, SPRY
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1, and encodes a shorter protein (isoform b).