GH2 (NM_002059) Human Untagged Clone

CAT#: SC109272

GH2 (untagged)-Human growth hormone 2 (GH2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GH2
• Synonyms: GH-V; GHB2; GHL; GHV; hGH-V
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109272 sequence for NM_002059 edited (data generated by NextGen Sequencing)
  ATGGCTGCAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGTCCTGG
  CTTCAAGAGGGCAGTGCCTTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATG
  CTCCGCGCCCGTCGCCTGTACCAGCTGGCATATGACACCTATCAGGAGTTTGAAGAAGCC
  TATATCCTGAAGGAGCAGAAGTATTCATTCCTGCAGAACCCCCAGACCTCCCTCTGCTTC
  TCAGAGTCTATTCCAACACCTTCCAACAGGGTGAAAACGCAGCAGAAATCTAACCTAGAG
  CTGCTCCGCATCTCCCTGCTGCTCATCCAGTCATGGCTGGAGCCCGTGCAGCTCCTCAGG
  AGCGTCTTCGCCAACAGCCTGGTGTATGGCGCCTCGGACAGCAACGTCTATCGCCACCTG
  AAGGACCTAGAGGAAGGCATCCAAACGCTGATGTGGAGGCTGGAAGATGGCAGCCCCCGG
  ACTGGGCAGATCTTCAATCAGTCCTACAGCAAGTTTGACACAAAATCGCACAACGATGAC
  GCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAG
  ACATTCCTGCGCATCGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAG
  
  Clone variation with respect to NM_002059.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002059 unedited
  NNNGGTTCAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCAAC
  TCCCCGAACCATCAGGTCCTGTGGACAGCTCACCTAGCGGCAATGGCTGCAGGCTCCCGG
  ACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGTCCTGGCTTCAAGAGGGCAGTGCC
  TTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATGCTCCGCGCCCGTCGCCTG
  TACCAGCTGGCATATGACACCTATCAGGAGTTTGAAGAAGCCTATATCCTGAAGGAGCAG
  AAGTATTCATTCCTGCAGAACCCCCAGACCTCCCTCTGCTTCTCAGAGTCTATTCCAACA
  CCTTCCAACAGGGTGAAAACGCAGCAGAAATCTAACCTAGAGCTGCTCCGCATCTCCCTG
  CTGCTCATCCAGTCATGGCTGGAGCCCGTGCAGCTCCTCAGGAGCGTCTTCGCCAACAGC
  CTGGTGTATGGCGCCTCGGACAGCAACGTCTATCGCCACCTGAAGGACCTAGAGGAAGGC
  ATCCAAACGCTGATGTGGAGGCTGGAAGATGGCAGCCCCCGGACTGGGCAGATCTTCAAT
  CAGTCCTACAGCAAGTTTGACACAAAATCGCACAACGATGACGCACTGCTCAAGAACTAC
  GGGCTGCTCTACTGCTTCAGGAAGGACATGGACAANGTCGAGACATTCCTGCGCATCGTG
  CAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGCTGCCCGGGTGGCATCCCTGTGAC
  CCCTCCCCAGTGCCTCTCCTGGTCGTGGAAGGTGCTACTCCAGTGCCCACCAGCCTTGTC
  CTAATANAATTAAGTTGCATCANAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCNG
  TCATAGCTGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002059
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002059.3, NP_002050.1
• RefSeq Size: 821 bp
• RefSeq ORF: 654 bp
• Locus ID: 2689
• UniProt ID: P01242
• Cytogenetics: 17q23.3
• Domains: hormone
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) utilizes all five exons generating the predominant 22-kDa isoform (1).