Aquaporin 4 (AQP4) (NM_001650) Human Untagged Clone

CAT#: SC108945

AQP4 (untagged)-Human aquaporin 4 (AQP4), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aquaporin 4
• Synonyms: MIWC; WCH4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108945 sequence for NM_001650 edited (data generated by NextGen Sequencing)
  ATGAGTGACAGACCCACAGCAAGGCGGTGGGGTAAGTGTGGACCTTTGTGTACCAGAGAG
  AACATCATGGTGGCTTTCAAAGGGGTCTGGACTCAAGCTTTCTGGAAAGCAGTCACAGCG
  GAATTTCTGGCCATGCTTATTTTTGTTCTCCTCAGCCTGGGATCCACCATCAACTGGGGT
  GGAACAGAAAAGCCTTTACCGGTCGACATGGTTCTCATCTCCCTTTGCTTTGGACTCAGC
  ATTGCAACCATGGTGCAGTGCTTTGGCCATATCAGCGGTGGCCACATCAACCCTGCAGTG
  ACTGTGGCCATGGTGTGCACCAGGAAGATCAGCATCGCCAAGTCTGTCTTCTACATCGCA
  GCCCAGTGCCTGGGGGCCATCATTGGAGCAGGAATCCTCTATCTGGTCACACCTCCCAGT
  GTGGTGGGAGGCCTGGGAGTCACCATGGTTCATGGAAATCTTACCGCTGGTCATGGTCTC
  CTGGTTGAGTTGATAATCACATTTCAATTGGTGTTTACTATCTTTGCCAGCTGTGATTCC
  AAACGGACTGATGTCACTGGCTCAATAGCTTTAGCAATTGGATTTTCTGTTGCAATTGGA
  CATTTATTTGCAATCAATTATACTGGTGCCAGCATGAATCCCGCCCGATCCTTTGGACCT
  GCAGTTATCATGGGAAATTGGGAAAACCATTGGATATATTGGGTTGGGCCCATCATAGGA
  GCTGTCCTCGCTGGTGGCCTTTATGAGTATGTCTTCTGTCCAGATGTTGAATTCAAACGT
  CGTTTTAAAGAAGCCTTCAGCAAAGCTGCCCAGCAAACAAAAGGAAGCTACATGGAGGTG
  GAGGACAACAGGAGTCAGGTAGAGACGGATGACCTGATTCTAAAACCTGGAGTGGTGCAT
  GTGATTGACGTTGACCGGGGAGAGGAGAAGAAGGGGAAAGACCAATCTGGAGAGGTATTG
  TCTTCAGTATGA
  
  Clone variation with respect to NM_001650.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001650 unedited
  NNNNNNNNNNNGGTTCAAATTTTGTNATACGACTCATATAGGCGGCCGCGNATTCGGCAC
  GAGGCCTCGTGCCGAATTCGGCACGAGGCTGCACTCTGGCTGGGGAAGGCATGAGTGACA
  GACCCACAGCAAGGCGGTGGGGTAAGTGTGGACCTTTGTGTACCAGAGAGAACATCATGG
  TGGCTTTCAAAGGGTCTGGACTCAAGCTTTCTGGAAAGCAGTCACAGCGGAATTTCTGGC
  CATGCTTATTTTTGTTCTCCTCAGCCTGGGATCCACCATCAACTGGGGTGGAACAGAAAA
  GCCTTTACCGGTCGACATGGTTCTCATCTCCCTTTGCTTTGGACTCAGCATTGCAACCAT
  GGTGCAGTGCTTTGGCCATATCAGCGGTGGCCACATCAACCCTGCAGTGACTGTGGCCAT
  GGTGTGCACCAGGAAGATCAGCATCGCCAAGTCTGTCTTCTACATCGCAGCCCAGTGCCT
  GGGGGCCATCATTGGAGCAGGAATCCTCTATCTGGTCACACCTCCCAGTGTGGTGGGGAG
  GCCCTGNGAGTCACCATGGTTCATGGAAATCTTACCGCTGGTCATGGTCTCCTGGNNTGA
  GNTGATAATCACATTTCAATTGGGTGTTTACTANTCTTTGCCAGCTGTGATTCCAAACGG
  ACTGATGTCACTGGCTCAATAGCTNTAGCATTTGGATTTTCTGGGTGCATTGGACATTTT
  TATTTGCATCAATTATACTGGNTGCCAGCATGAATCCCGCCCGATCCTTTTGACCTGCAG
  TTATCATGGGGAAATTGGGAAAAACATTGATATATTGTGGGGTTTGGGCCCATCATAGGG
  AGCTGTCCCTCCCTGGTGGGCTTATGGAAGTATGGTCTCTGTCCCAAAGGTTGGATTCAA
  AACGTCGGTTTTAAAGAAACCCTTCAGCCAAGCTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001650 unedited
  NAAATTGACTTGNNACCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTTTTTTTTTACC
  TTTTAAAACATCCATGTTTATTTTCACAAGCTTAGTAGTACAAGATGCAACATAATTCAA
  GAAAATCAACCACAGTGCTAATACTTTAATATATTATTCCAAATATTAGACGTTACAGTT
  TAAATCTTTTTAACATTAGCTCTAATGTTGGAATAACATTTAAGTTAATAAATAACTGTG
  AATATAACCATATGTTCAGTCTATTCTCCCCAGTTTATCCTAAATGACAATTTTATTGTG
  ATTTGGGTCATAAAAGTGATAAATAATTGTTTATATGTTTTCATAAATTGCATGTTTATT
  TAATTAATCCTGAAGAGAACCTAGAAATACATTCCAATGGGTTACATAAATTAGACGTAT
  TTTTTAGCTCTTCGATTTTTTTTAAACATGAGTGAGTCACACTGTAACTGCTCTTAAATT
  TAGTGTTCATTGCACATAACATTTTTTGAAATGTATTTTCACAGGCTATAGGTAGTCATT
  TGCAAAGATGGCCACAATTTTTGATCCTTGAATTAAATGTCTTTCATTTATTTCAGAGAA
  TTATGAGTTTCAAAAGCTACTGCTCTTATGGGGCAATCTGTAGAGTACTAAACTCACAAA
  ATTTGTGGTAACAAAAGAGAGTTTTGTTACATTACACTTTCCAAATAGTAACCACATCTT
  CCTACTAATTTTCATGTTAAAAACACTTTAGGAAAGACAGAATTACTTTTAGGTATAAAA
  TGTTCTGAATTTGCAAATTCTATAGTGCTTATGAGAAATTATATTGGGCTTTTATATTGT
  TTTGATAAAGTTGTCTGGTGCCCCCCAAACTTCCATCTTCAGTTGGCACCAAAGCAAATT
  CTCTGTGGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001650
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001650.4, NP_001641.1
• RefSeq Size: 5216 bp
• RefSeq ORF: 972 bp
• Locus ID: 361
• UniProt ID: P55087
• Cytogenetics: 18q11.2
• Domains: MIP
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018]
  Transcript Variant: This variant (1, also known as hMIWC2) represents the predominant transcript and encodes four isoforms, resulting from the use of two alternative in-frame translation initiation codons and two alternative in-frame translation termination codons. This RefSeq represents isoform M1, which results from the use of the upstream AUG start codon and the upstream UGA stop codon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.