POLR1D (NM_015972) Human Untagged Clone

CAT#: SC108711

POLR1D (untagged)-Human polymerase (RNA) I polypeptide D, 16kDa (POLR1D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR1D
• Synonyms: AC19; POLR1C; RPA9; RPA16; RPAC2; RPC16; RPO1-3; TCS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015972, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGAGGATCAGGAGCTGGAGAGAAAAATATCTGGATTGAAGACCTCAATGGCTGAAGGCGAGAGGA
  AGACAGCCCTGGAAATGGTCCAGGCAGCTGGAACAGATAGACACTGTGTGACATTTGTATTGCACGAGGA
  AGACCATACCCTAGGAAATTCTCTACGTTACATGATCATGAAGAACCCGGAAGTGGAATTTTGTGGTTAC
  ACTACGACCCATCCTTCAGAGAGCAAAATTAATTTACGCATTCAGACTCGAGGTACCCTTCCAGCTGTTG
  AGCCATTTCAGAGAGGCCTGAATGAGCTCATGAATGTCTGCCAACATGTGCTTGACAAGTTTGAGGCCAG
  CATAAAGGACTATAAGGATCAAAAAGCAAGCAGAAATGAATCCACATTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015972 unedited
  GTCAGATTTGTATACGACTTCTATAGGCGGCCGCGCAATTCGCACGAGGTTCCGTCGGTC
  GGTCCTTGCTTCCTGCTTCGCCTCCGCGCCTCGCGCTATGGGACAGAGCCCCCGATCCGC
  CAGCACCACCTGAGGATCCAGAAACCGCCCCAGCGATGGAAGAGGATCAGGAGCTGGAGA
  GGAAAGCAATAGAAGAACTGCTTAAGGAGGCAAAACGTGGGAAAACTAGAGCTGAAACAA
  TGGGACCCATGGGTTGGATGAAGTGTCCTCTTGCTAGCACCAATAAAAGATTTCTAATTA
  ACACAATTAAAAACACATTGCCCTCTCATAAAGAGCAAGACCATGAACAAAAAGAGGGCG
  ATAAGGAACCAGCCAAGAGCCAGGCCCAGAAAGAAGAAAACCCAAAGAAACACAGAAGCC
  ATCCTTACAAGCACAGCTTCCGCGCTCGAGGTTCCGCCAGTTACTCCCCGCCACGAAAGC
  GGAGCAGCCAGGACAAGTACGAAAAGCGGTCCAACCGGCGGTGAGGCTGGAACCAGGGCC
  AGCCAGCCCTGCGGGCCTCCGTGCTCCTTCGGGGTGCGGTGTGCGGAGAAGGCCTGAGCT
  GGCAGGGCAAACACCTGAGAGTGACTTTGACATTAGGTCCTTTGTTGTTTTTAAAGAGGC
  TGCTGCCCACAGGCAAAAAAAATGACCTAGCAACTCTTGAGGAAACAAGTGCAAAGGGAG
  ATGTCTTGGCAAGTTTAAGCTACTTCTGCAGTGTAGTAGACTTACATATTGNTGNTTATC
  TTGGTTGGAGTGTGTTTAAAATAAACTGTCTAGAATTAAAAGTTTCAGAGATTGGTAAAC
  CTGGTCCCAATTTTTGGTCATTTCTCAGAAAATAAATACCTATAATCCTTATTTTACTTT
  TGGGGGTATTACATTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015972
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015972.1, NP_057056.1
• RefSeq Size: 726 bp
• RefSeq ORF: 402 bp
• Locus ID: 51082
• UniProt ID: P0DPB6
• Cytogenetics: 13q12.2
• Domains: RNA_pol_L
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (1) represents the shortest transcript but encodes the longest isoform (1).