ARIH2 (NM_006321) Human Untagged Clone

CAT#: SC108565

ARIH2 (untagged)-Human ariadne homolog 2 (Drosophila) (ARIH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARIH2
• Synonyms: ARI2; TRIAD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006321 edited
  GAATTCGGCACGAGGCACTTCCGGAGCTGTGGGGACGACTCTTCTGGAGGAAGCAGCGCG
  GGCTTGACCGGCGTCGGCCCGCCGCCTCCGCTGCCGCTTCGCCCCAATCCGGTCCCTCTG
  GCCCGGCCTGACCCGGTCTGGCTTGTTCGGGCTCAGCGGCCGCGAGGCCGCAGCTCCCGA
  TGGAAATCATATTATGTAGAATACTTGGGTGACATCTGCCTGAGAGATCTCCAAGAATTA
  CAGATTGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCTGCTCACTG
  CAACCTCCGTCTCCCGAGTTCAAGCAGTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGAT
  TACAGGTAAGACAAAAATACTAATGCATTTGAGAAAGCGGTAGTTTTGGGGGGAGGGGGA
  AAAAGCAACTGCTTTCCTGATCTGCAACTTGGCTGGATGCTAAGATGTCAGTGGACATGA
  ATAGCCAGGGGTCTGACAGCAATGAAGAGGACTATGACCCAAATTGTGAGGAAGAGGAAG
  AAGAAGAAGAAGACGACCCTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATG
  TGGAGCAGCAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGACCT
  ACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTTCTGTCCTAAAGG
  TATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTCCACTGGCAAGTTTCAGAGATAT
  TGGACAGATACAAGTCCAATTCTGCTCAACTGCTTGTTGAGGCTCGAGTTCAGCCTAATC
  CATCAAAACATGTTCCCACATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTG
  TGCGAAAGGAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTGGG
  AGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCATGGCTC
  AGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGCTTCCCAATGAAGAAT
  TGAGAGAGAAATACAGGCGCTACCTCTTCAGGGACTATGTGGAGAGTCATTACCAGCTCC
  AGCTGTGCCCTGGTGCAGACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCC
  GAGTACAGTGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCACG
  CACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAGACGACTCTGAAA
  CAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCATTGAGA
  AGAATGGAGGCTGCAATCACATGCAATGCTCCAAATGTAAACACGACTTCTGCTGGATGT
  GTCTAGGAGATTGGAAGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGA
  ATCCTGACATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACT
  TATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACAGACAT
  ACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATCTGGGGACATGGATCG
  ACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGTCGATACACCCTGCAAT
  ACACCTACCCATATGCATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACC
  AGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACA
  GCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCC
  TGCTGAAAGATTTCCATGACACCTAAGTTGGGATGTGGATGTGCCGGGGTGAGGAAGATG
  TGGCTGCAAGGTCTCCCGGCTGCCATACTGCATGCTGCAGGCTCTGCCTTTCATGACCCC
  AGGCAACAGCCAGGGCCCCACTCCTGAGAGACACTGGCAACACCTCTTAGTTGATTTCTG
  TTTTCTTCTCTTTTCACTTTTTGTTTCTACCAGGGTAGAGGCCATGTTGAACTGGCCTCT
  TTTCAGGACTTTTATTTCCCCCTGGATGGTTGTTGGGAGGGAGGGAAAGTGTTTTCTGAA
  TGGCTATTAATAGTATTAGATCATTACAACTTATGTAACTTTCAAAGGTTGTACAATTAT
  ACAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006321 unedited
  TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCACTTCCGGAGCT
  GTGGGGACGACTCTTCTGGAGGAAGCAGCGCGGGCTTGACCGGCGTCGGCCCGCCGCCTC
  CGCTGCCGCTTCGCCCCAATCCGGTCCCTCTGGCCCGGCCTGACCCGGTCTGGCTTGTTC
  GGGCTCAGCGGCCGCGAGGCCGCAGCTCCCGATGGAAATCATATTATGTAGAATACTTGG
  GTGACATCTGCCTGAGAGATCTCCAAGAATTACAGATTGAGTCTCGCTCTGTTGCCCAGG
  CTGGAGTGCAGTGGTGCAATCTCTGCTCACTGCAACCTCCGTCTCCCGAGTTCAAGCAGT
  TCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTAAGACAAAAATACTAATGCAT
  TTGAGAAAGCGGTAGTTTTGGGGGGAGGGGGAAAAAGCAACTGCTTTCCTGATCTGCAAC
  TTGGCTGGATGCTAAGATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
  GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACCCTGGGGACAT
  AGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAGCAGGGGGCTGATGCCTTTGA
  TCCCGAGGAGTACCAGNTCACTTGCTTGACCTACAAGGAATCTGAGGGTGCCCTCAATGA
  GCACATGACCAGCTTAGCTTCTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATT
  AGTTAATTTCCCACTGGCAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
  ACTGCCTGTTGAGCCTCCAGNTCACCCTAACCATCAAAAATGTTCCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006321 unedited
  CAGAGTCGAGTTTTTTTTTTTTTTTTTTGTATAATTGTACAACCTTTTGAAAGTTACATA
  AGTTGTAATGATCTAATACTATTAATAGCCATTCAGAAAACACTTTCCCTCCCTCCCAAC
  AACCATCCAGGGGGAAATAAAAGTCCTGAAAAGAGGCCAGTTCAACATGGCCTCTACCCT
  GGTAGAAACAAAAAGTGAAAATAGAAGAAAACAGAAATCAACTAAGAGGTGTTGCCAGTG
  TCTCTCAGGAGTGGGGCCCTGGCTGTTGCCTGGGGTCATGAAAGGCAGAGCCTGCAGCAT
  GCAGTATGGCAGCCGGGAGACCTTGCAGCCACATCTTCCTCACCCCGGCACATCCACATC
  CCAACTTAGGTGTCATGGAAATCTTTCAGCAGGGTTCTCCTCCGCTGCTCCGCTATATGC
  ATCTGGTTCTCCAAGTCCCCTCTGTCATAGCTGTCTGCACCCTCCACTTTCCATGACAGG
  TTTTCGATTCTCAGCTCCACCTGAGCCTGCTGGTATTCAAACAGCTTCTTCCTGGGTCCC
  GACTCCTTGTAATATGCATATGGGTACGGTGTCTTGCAGGTTGTTTCGACACTTGGCCCA
  GAACTTTGGACCATTTCTGTAGGCACTGCCAGTTGTATCTTGTCCCCAAGATGTCCATGA
  CCCTCCTCTGAATCTTCCTCTGAACCCGGTGGATTTTCTGGCCTTCACCTCTAACCTTTA
  TTGGGTTTTCTCCCACTCCCCCCCTAAAACAATCACTCTTGTATGGCTCTCCTTTCCCGG
  TTCTGTCGGTTTGGTCCACCATTAACGACTTCCCCTTGTACACCCGCCCATATTGTACCC
  ATGCCTGCTCCTCCCCCCTCTTTTTACCCTCCCCCTAACCGCCGTCTCCTCTCGCCTCCT
  CTTGTCCCGTCCGTTCTTCTTCTCATGTCCATTTTCTCCCGTATCTTTTCTCCCGTTTAC
  CTTGTTTCCGCCTCTCCATATATCTTTCCCTTCCCGACCTATTTTCTCCTCGCCCCTCTT
  GTTTGTTTCACCTTACACTTCGAACACCAAATTTCTTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006321
• Insert Size: 2490 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006321.1, NP_006312.1
• RefSeq Size: 3892 bp
• RefSeq ORF: 1482 bp
• Locus ID: 10425
• UniProt ID: O95376
• Cytogenetics: 3p21.31
• Domains: IBR
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (2), as well as variants 1, 4, 5, and 6, encodes isoform a.