ATP5MC1 (NM_005175) Human Untagged Clone

CAT#: SC108539

ATP5G1 (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9) (ATP5G1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5MC1
• Synonyms: ATP5A; ATP5G; ATP5G1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005175 edited
  ATGCAGACCGCCGGGGCATTATTCATTTCTCCAGCTCTGATCCGCTGTTGTACCAGGGGT
  CTAATCAGGCCTGTGTCTGCCTCCTTCTTGAATAGCCCAGTGAATTCATCTAAACAGCCT
  TCCTACAGCAACTTCCCACTCCAGGTGGCCAGACGGGAGTTCCAGACCAGTGTTGTCTCC
  CGGGACATTGACACAGCAGCCAAGTTTATTGGTGCTGGGGCAGCCACAGTTGGTGTGGCT
  GGTTCAGGGGCTGGCATTGGAACCGTGTTTGGCAGCTTGATCATTGGCTATGCCAGGAAC
  CCGTCTCTCAAGCAGCAGCTCTTCTCCTATGCCATTCTTGGCTTTGCCCTGTCTGAGGCC
  ATGGGGCTTTTCTGTTTGATGGTCGCCTTCCTCATCCTCTTCGCCATGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005175 unedited
  CACGAGGGGCGAGCGCTGTGGGCCAAGCAGGGGTTGCAGGGCAGTAGGAGTGCAGACTGA
  AAAAAGCAGACCGCCGGGGCATTATTCATTTCTCCAGCTCTGATCCGCTGTTGTACCAGG
  GGTCTAATCAGGCCTGTGTCTGCCTCCTTCTTGAATAGCCCAGTGAATTCATCTAAACAG
  CCTTCCTACAGCAACTTCCCACTCCAGGTGGCCAGACGGGAGTTCCAGACCAGTGTTGTC
  TCCCGGGACATTGACACAGCAGCCAAGTTTATTGGTGCTGGGGCAGCCACAGTTGGTGTG
  GCTGGTTCAGGGGCTGGCATTGGAACCGTGTTTGGCAGCTTGATCATTGGCTATGCCAGG
  AACCCGTCTCTCAAGCAGCAGCTCTTCTCCTATGCCATTCTTGGCTTTGCCCTGTCTGAG
  GCCATGGGNGCTTTTCTGTTTGATGGTCGCCTTCCTCATCCTCTTCGCCATGTGAGGCTC
  CTGGNNGGGTCACCGGCCTGTTGCTACTGCAACTCCACACCTTCTTGGGCTGGGGGGTGG
  TGTTAAGCTTTACCATTAAACACAACGTTTCTCTAAAAAAAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005175 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTAAAAAAACGTTGTGTTTAATGGTAAAGCTTAACACACCCCAACACCAAA
  AATGGGGTGGAGTTGCAGTAACAACAGGCCGGGGACCCCCCATGGAGCCTCACATGGCGA
  AAAGGATGAGGAAGGCGACCTTTAAACAAAAAAGCCCCATGGCCTCAAACAGGGCAAAGC
  CAAAAATGGCATAGGAAAAAAACTGCTGCTTGAAAAACGGGTTCCTGGCATAACCAATGA
  TCAAGCTGCCAAACACGGTTCCAATGCCAGCCCCTGAACCAACCACACCAACTGGGGGTG
  CCCCAGCACCAATAAACTTGGCTGCTGTGTCAATGTCCCGGGAAACAACACTGGTCTGGA
  ACTCCCGTCTGGCCACCTGGAGTGGGAAATTGCTGTAAGAAGGCTGTTTAAATGAATTCA
  CTGGGCTATTCAAGAAGGAGGCAGACACAGGCCTGATTAAACCCCTGGTACAACAGCGGA
  TCAGAGCTGGAGAAATGAATAATGCCCCGGCGGTCTGCATTTTTTCAGTTTGGACTCCTA
  CTGGCCTGTAACCCCTGTTTGGCCCAAAGGGCTCGCCCCTTGTGCCGAATTCGCGGCCCG
  CCCTATAGTGAGTCGTATTACAAAATTTTGACGGTTCAATAAACAACCTCTGTTTTTTAG
  ACCCTCCACCGTACACGCCTACCGCCCCTTTTGCGTTAACGGGGGCGGGGGTTATTACCA
  CATTTTTGAAAGTCCCGGTTGTATTTTGTGCCAAAACAAACTTCCTCTTGACGTAAATGG
  GTGGGGGAACTTGGAAATCCCGCGGTGGTACAAACGCGTTATCCCCGCCTTTTGGGGTTC
  TGTGCAAAACCGTTTAACTTTGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005175
• Insert Size: 680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005175.2, NP_005166.1
• RefSeq Size: 663 bp
• RefSeq ORF: 411 bp
• Locus ID: 516
• UniProt ID: P05496
• Cytogenetics: 17q21.32
• Domains: ATP-synt_C
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.