HB EGF (HBEGF) (NM_001945) Human Untagged Clone

CAT#: SC108485

HBEGF (untagged)-Human heparin-binding EGF-like growth factor (HBEGF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HB EGF
• Synonyms: DTR; DTS; DTSF; HEGFL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001945 edited
  GAATTCGGCACGAGGGCTACGCGGGCCACGCTGCTGGCTGGCCTGACCTAGGCGCGCGGG
  GTCGGGCGGCCGCGCGGGCGGGCTGAGTGAGCAAGACAAGACACTCAAGAAGAGCGAGCT
  GCGCCTGGGTCCCGGCCAGGCTTGCACGCAGAGGCGGGCGGCAGACGGTGCCCGGCGGAA
  TCTCCTGAGCTCCGCCGCCCAGCTCTGGTGCCAGCGCCCAGTGGCCGCCGCTTCGAAAGT
  GACTGGTGCCTCGCCGCCTCCTCTCGGTGCGGGACCATGAAGCTGCTGCCGTCGGTGGTG
  CTGAAGCTCTTTCTGGCTGCAGTTCTCTCGGCACTGGTGACTGGCGAGAGCCTGGAGCGG
  CTTCGGAGAGGGCTAGCTGCTGGAACCAGCAACCCGGACCCTCCCACTGTATCCACGGAC
  CAGCTGCTACCCCTAGGAGGCGGCCGGGACCGGAAAGTCCGTGACTTGCAAGAGGCAGAT
  CTGGACCTTTTGAGAGTCACTTTATCCTCCAAGCCACAAGCACTGGCCACACCAAACAAG
  GAGGAGCACGGGAAAAGAAAGAAGAAAGGCAAGGGGCTAGGGAAGAAGAGGGACCCATGT
  CTTCGGAAATACAAGGACTTCTGCATCCATGGAGAATGCAAATATGTGAAGGAGCTCCGG
  GCTCCCTCCTGCATCTGCCACCCGGGTTACCATGGAGAGAGGTGTCATGGGCTGAGCCTC
  CCAGTGGAAAATCGCTTATATACCTATGACCACACAACCATCCTGGCCGTGGTGGCTGTG
  GTGCTGTCATCTGTCTGTCTGCTGGTCATCGTGGGGCTTCTCATGTTTAGGTACCATAGG
  AGAGGAGGTTATGATGTGGAAAATGAAGAGAAAGTGAAGTTGGGCATGACTAATTCCCAC
  TGAGAGAGACTTGTGCTCAAGGAATCGGCTGGGGACTGCTACCTCTGAGAAGACACAAGG
  TGATTTCAGACTGCAGAGGGGAAAGACTTCCATCTAGTCACAAAGACTCCTTCGTCCCCA
  GTTGCCGTCTAGGATTGGGCCTCCCATAATTGCTTTGCCAAAATACCAGAGCCTTCAAGT
  GCCAAACAGAGTATGTCCGATGGTATCTGGGTAAGAAGAAAGCAAAAGCAAGGGACCTTC
  ATGCCCTTCTGATTCCCCTCCACCAAACCCCACTTCCCCTCATAAGTTTGTTTAAACACT
  TATCTTCTGGATTAGAATGCCGGTTAAATTCCATATGCTCCAGGATCTTTGACTGAAAAA
  AAAAAAGAAGAAGAAGAAGGAGAGCAAGAAGGAAAGATTTGTGAACTGGAAGAAAGCAAC
  AAAGATTGAGAAGCCATGTACTCAAGTACCACCAAGGGATCTGCCATTGGGACCCTCCAG
  TGCTGGATTTGATGAGTTAACTGTGAAATACCACAAGCCTGAGAACTGAATTTTGGGACT
  TCTACCCAGATGGAAAAATAACAACTATTTTTGTTGTTGTTGTTTGTAAATGCCTCTTAA
  ATTATATATTTATTTTATTCTATGTATGTTAATTTATTTAGTTTTTAACAATCTAACAAT
  AATATTTCAAGTGCCTAGACTGTTACTTTGGCAATTTCCTGGCCCTCCACTCCTCATCCC
  CACAATCTGGCTTAGTGCCACCCACCTTTGCCACAAAGCTAGGATGGTTCTGTGACCCAT
  CTGTAGTAATTTATTGTCTGTCTACATTTCTGCAGATCTTCCGTGGTCAGAGTGCCACTG
  CGGGAGCTCTGTATGGTCAGGATGTAGGGGTTAACTTGGTCAGAGCCACTCTATGAGTTG
  GACTTCAGTCTTGCCTAGGCGATTTTGTCTACCATTTGTGTTTTGAAAGCCCAAGGTGCT
  GATGTCAAAGTGTAACAGATATCAGTGTCTCCCCGTGTCCTCTCCCTGTCAAGTCTCAGA
  AGAGGTTGGGCTTCCATGCCTGTAGCTTTCCTGGTCCCTCACCCCCATGGCCCCAGGCCC
  ACAGCGTGGGAACTCACTTTCCCTTGTGTCAAGACATTTCTCTAACTCCTGCCATTCTTC
  TGGTGCTACTCCATGCAGGGGTCAGTGCAGCAGAGGACAGTCTGGAGAAGGTATTAGCAA
  AGCAAAAGGCTGAGAAGGAACAGGGAACATTGGAGCTGACTGTTCTTGGTAACTGATTAC
  CTGCCAATTGCTACCGAGAAGGTTGGAGGTGGGGAAGGCTTTGTATAATCCCACCCACCT
  CACCAAAACGATGAAGTTATGCTGTCATGGTCCTTTCTGGAAGTTTCTGGTGCCATTTCT
  GAACTGTTACAACTTGTATTTCCAAACCTGGTTCATATTTATACTTTGCAATCCAAATAA
  AGATAACCCTTATTCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001945 unedited
  NNNGGTTCGCATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGC
  TACGCGGGCCACGCTGCTGGCTGGCCTGACCTAGGCGCGCGGGGTCGGGCGGCCGCGCGG
  GCGGGCTGAGTGAGCAAGACAAGACACTCAAGAAGAGCGAGCTGCGCCTGGGTCCCGGCC
  AGGCTTGCACGCAGAGGCGGGCGGCAGACGGTGCCCGGCGGAATCTCCTGAGCTCCGCCG
  CCCAGCTCTGGTGCCAGCGCCCAGTGGCCGCCGCTTCGAAAGTGACTGGTGCCTCGCCGC
  CTCCTCTCGGTGCGGGACCATGAAGCTGCTGCCGTCGGTGGTGCTGAAGCTCTTTCTGGC
  TGCAGTTCTCTCGGCACTGGTGACTGGCGAGAGCCTGGAGCGGCTTCGGAGAGGGCTAGC
  TGCTGGAACCAGCAACCCGGACCCTCCCACTGTATCCACGGACCAGCTGCTACCCCTAGG
  AGGCGGCCGGGACCGGAAAGTCCGTGACTTGCAAGAGGCAGATCTGGACCTTTTGAGAGT
  CACTTTATCCTCCAAGCCACAAGCACTGGCCACACCAAACAAGGAGGAGCACGGGAAAAG
  AAAGAAGAAAGGCAAGGGGCTAGGGAAGAAGAGGGACCCATGTCTTCGGAAATACAAGGA
  CTTCTGCATCCATGGAGAATGCANATATGTGAAGGAGCTCCGGGCTCCCTCCTGCATCTG
  CCACCCGGGTTACCATGGAGAGAGGTGTCATGGGCTGAGCCTNCCAGTGGAAAATCGCTT
  ATATACCTATGACCACACACCATCCTGGCCGTGGTGGCTGTGGTGCTGTCATCTGTCTGT
  CTGCTGGTCATCGTGGGGCTTNCTCATGTTAGGTACCCATAGAGAGGGAGTNATGATGTN
  GNAAATGAAGAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001945 unedited
  GGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGAATAAGGGTTTTTTTTTTTTGGATTGCAA
  AGTTTAAATATGAACCAGGTTTGGAAATACAAGTTGTAACAGTTCAAAAATGGCCCCAAA
  AACTTCCAAAAAGGACCCTGACAGCATAACTTCTTTGTTTTGGGGAGGGGGGGGGGATTA
  TACAAAGCCTTCCCCACCTTCAACCTTTTTGGGAGCAATTGGGAGGTAATTAGTTACCAA
  AAACAGGCAGCTCCAATGTTCCCTGTTCCTTTTAAACCTTTTGGTTTGGTAAAACCTTTT
  CCAAAATGTCCTTTGGTGGACTGACCCCTGGTTGGGGTACCCCCAAAAAAATGGGAGGAG
  TTAAAAAAATGTCTTGACACAAGGGAAAGGGAGTTCCCACCCTGGGGGCCTGGGGCCATG
  GGGGGGAGGGACCAAAAAAGCTACAGGCTTGGAAGCCCAACCTTTTTTGAAACTTGACAG
  GGAGAGGACACGGGGAAACACTGATTTTTGTTACACTTTGACATCAACACCTTGGGCTTT
  AAAAACACAAATGGGAGACAAAATCGCCTAGGGAAGACTGAAGTCCAACTCATAGAGGGG
  CTCTGACCAAGTTAACCCCTACATTCTGACCATACAAAGCTCCCGCAGTGGCACTTTGAC
  CCCGAAGATCTGCAAAATTGTTAACGGACAATTAATTTCTACAGATGGGTCCACAGACCA
  TCCTTACCTTTGTGCGAATGTGGGTGGCACTATACCATATTGGGGGATATAGATTGAAGG
  CCCCNAAAATGCCAAAGAACAGCTAGGCCCTTAAATTTATGGTAATTGGNAAACCTCATA
  TTTACCTCTTGAATAATAAATTTTTTTTAAGGGGCTTCCAACACACCACCAATTTTGTTA
  TTTCCTCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001945
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001945.1, NP_001936.1
• RefSeq Size: 2360 bp
• RefSeq ORF: 627 bp
• Locus ID: 1839
• UniProt ID: Q99075
• Cytogenetics: 5q31.3
• Domains: EGF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, GnRH signaling pathway
• Gene Summary: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.[UniProtKB/Swiss-Prot Function]